Apoptosis-Inducing Activity of HPLC Fraction from Voacanga globosa (Blanco) Merr. on the Human Colon Carcinoma Cell Line, HCT116

Abstract

likely to produce anti-cancer compounds. Using MTT assay, the study aimed to test HPLC fractions from the V globosa leaf extract for cytotoxicity against the HCT116 human colon carcinoma, A549 human lung carcinoma and the non-cancer AA8 Chinese hamster ovarian cell lines. Possible pro-apoptotic activity was also assessed using DAPI nuclear staining, TUNEL assay and the JC-1 mitochondrial membrane potential assay. Production of the ethanolic crude extractMature leaves from V globosa (Blanco) Merr. wereInstitute of Biology, University of the Philippines Diliman (a voucher specimen sample submitted was assigned the accession number: 14609). Leaves were air dried until crispy and then macerated in 95% technical grade ethanol

Cytogenotoxic effects of Adenium obesum seeds extracts on breast cancer cells

“…This was supported by translocation of PS to outer surface of PM (Fadok et al, 1998) under microscopy, the DNA damage (Nagata, 2000) induced in comet assay also clearly suggest that the seed extract has therapeutic value against cancer cells. The anti-cancer potential of Carissa spinarum (Sehar et al, 2011, Rao et al, 2005), Wrightia tomentosa (Chakravarti et al, 2012) and several other members of Apocynaceae have also previously been documented (Acebedo et al, 2014).…”

Luteolin, a Bioflavonoid Inhibits Colorectal Cancer through Modulation of Multiple Signaling Pathways: A Review

“…An increase in Bax relative to Bcl-2 promotes release of cytochrome C from the mitochondria with subsequent activation of caspase-3, thereby inducing mitochondrial mediated apoptosis (Gupta and DuBois, 2001). Natural compounds has the ability to induce cytotoxicity thereby protects against cancer and many researcher's developing chemotherapeutic by based on its ability to induce apoptosis (Sriram et al, 2008;Acebedo et al, 2014;Shafie et al, 2013b;Zou et al, 2013). Lim et al (2012) reported that luteolin induce cell cycle arrest and apoptosis in HT-29 human colon cancer cells.…”

Lack of Relation of AKAP12 with p53 and Bcl-2 in Colorectal Carcinoma

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