Apoptosis-like bacterial death modulated by photoactive hyperthermia nanomaterials and enhanced wound disinfection application

Li, Qiang; Jin, Hangshuai; Feng, Yonghai; Wu, Rongrong; Song, Yufei; Liu, Lei

doi:10.1039/d1nr02881b

Cited by 22 publications

(14 citation statements)

References 24 publications

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“…It is well-known that bacteria are composed of a variety of lipid types . The bacterial lipid metabolite changes convey much valuable information on the interactions between antibacterial treatment and bacteria. , Previous reports revealed that the high temperature in conventional PTT could disrupt the enzyme activity and phospholipids on the cell membrane. , The activity of antibacterial agents also depends on their interaction with bacterial membranes . However, there are few investigations about the change of lipid molecular pattern when treated with different therapy.…”

Section: Resultsmentioning

confidence: 99%

“…64,65 Previous reports revealed that the high temperature in conventional PTT could disrupt the enzyme activity and phospholipids on the cell membrane. 66,67 The activity of antibacterial agents also depends on their interaction with bacterial membranes. 62 However, there are few investigations about the change of lipid molecular pattern when treated with different therapy.…”

Section: Loading and Antioxidant Activity Of Cur In Cuppsimentioning

confidence: 99%

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Porous Silicon Carrier Endowed with Photothermal and Therapeutic Effects for Synergistic Wound Disinfection

Duan

Liu

Zhao

et al. 2022

ACS Appl. Mater. Interfaces

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Drug carriers endowed with photothermal effects will allow the drug delivery system to release drugs in a thermal-stimuli manner. In addition, the photothermal therapy (PTT) will also interplay with therapeutic drugs loaded in the carrier to exhibit synergistic bioactivity for various disease treatment. However, endowing the drug carrier with photothermal and synergistic therapeutic effects still has challenge. Herein, we demonstrate that surface modification of porous silicon (PSi) with polydopamine (PDA) could endow the classical drug carrier with a significant photothermal effect for advanced antibacterial therapy and wound disinfection. Specifically, the PSi surface interacts with a Cu2+/PDA complex via a simple and fast surface reduction-induced deposition method, forming the unique CuPDA coated PSi microcarrier (CuPPSi) without blocking the mesoporous structure. The CuPPSi carrier generates a higher near-infrared (NIR) photothermal efficiency and improved drug loading capacity owing to the abundant functional groups of PDA. Stimuli-responsive release of antibacterial Cu2+ and loaded curcumin (Cur) from CuPPSi can be realized under multiple stimuli including pH, reactive oxygen species and NIR laser irradition. Benefited from the carrier’s intrinsic multimodal therapy, the CuPPSi-Cur platform exhibits amplified, broad-spectrum, and synergistic antibacterial effect, killing more than 98% for both Staphylococcus aureus and Escherichia coli at a mild PTT temperature (∼45 °C). Notably, the combined therapy promotes migration of fibroblasts with no significant cytotoxicity as revealed through cell experiments in vitro. In bacteria-infected mice model, efficient bacterial ablation and wound healing are further demonstrated with negligible side effects in vivo. Overall, the rational design of a drug carrier with photothermal and therapeutic effects provides a novel intervention for amplifing wound disinfection clinically.

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Section: Resultsmentioning

confidence: 99%

Section: Loading and Antioxidant Activity Of Cur In Cuppsimentioning

confidence: 99%

Porous Silicon Carrier Endowed with Photothermal and Therapeutic Effects for Synergistic Wound Disinfection

Duan

Liu

Zhao

et al. 2022

ACS Appl. Mater. Interfaces

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“…Comparing with visible light, NIR with wavelength ranging from 780 to 2500 nm is featured with deeper tissue penetration and invasiveness [98]. Thus, NIR-based PTT has been extensively explored for anti-infective therapy [99][100][101]. The photothermal nanoagents can absorb the NIR and convert the optical energy into heat, which then initiates the cell membrane breakage and protein denaturation in bacteria, finally leading to their thermal ablation [102][103][104].…”

Section: Nir Stimulationmentioning

confidence: 99%

Efficient nanozyme engineering for antibacterial therapy

et al. 2022

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Antimicrobial resistance (AMR) has posed a huge threat to human health. It is urgent to explore efficient ways to suppress the spread of AMR. Antibacterial nanozymes has become one of the powerful weapons to combat AMR due to their enzyme-like catalytic activity with a broad-spectrum antibacterial performance. However, the inherent low catalytic activity of nanozymes limits their expansion into antibacterial applications. In this regard, a variety of advanced chemical design strategies have been developed to improve the antimicrobial activity of nanozymes. In this review, we have summarized the recent progress of advanced strategies to engineering efficient nanozymes for fighting against AMR, which can be mainly classified into catalytic activity improvement, external stimuli, bacterial affinity enhancement, and multifunctional platform construction according to the basic principles of engineering efficient nanocatalysts and the mechanism of nanozyme catalysis. Moreover, the deep insights into the effects of these enhancing strategies on the nanozyme structures and properties are highlighted. Finally, current challenges and future perspectives of antibacterial nanozymes are discussed for their future clinical potential.

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“…Specifically, gold nanorods (AuNRs) exhibit advantageous high photothermal conversion efficiency at the therapeutic window of the near-infrared (NIR) region, which is not absorbed by most living tissues [ 8 , 9 ]. The mechanism of APTT is still unraveling, with reports indicating thermal damage [ 10 ], as well as bacterial membrane disruption (but not necessarily rupture) [ 11 ], and even the localized formation of reactive oxygen species [ 12 ]. Furthermore, the bulk temperature elevation associated with APTT is ~20 °C lower than that required for simple thermal bacterial inactivation [ 11 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism of APTT is still unraveling, with reports indicating thermal damage [ 10 ], as well as bacterial membrane disruption (but not necessarily rupture) [ 11 ], and even the localized formation of reactive oxygen species [ 12 ]. Furthermore, the bulk temperature elevation associated with APTT is ~20 °C lower than that required for simple thermal bacterial inactivation [ 11 , 13 , 14 , 15 ]. The lower temperature required is advantageous, as excessive tissue heating and damage could be minimized [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Gold Nanorod-Incorporated Halloysite Nanotubes Functionalized with Antibody for Superior Antibacterial Photothermal Treatment

et al. 2022

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The global spread of antibiotic-resistant strains, and the need to protect the microflora from non-specific antibiotics require more effective and selective alternatives. In this work, we demonstrate for the first time a superior antibacterial photothermal effect of plasmonic gold nanorods (AuNRs) via their incorporation onto natural clay halloysite nanotubes (HNTs), which were functionalized with anti-E. coli antibodies (Ab-HNTs). AuNRs were incorporated onto the Ab-HNTs through a facile freeze–thaw cycle, and antibody integrity following the incorporation was confirmed via infrared spectroscopy and fluorescence immunolabeling. The incorporation efficiency was studied using UV-Vis absorption and transmission electron microscopy (TEM). Mixtures of E. coli and AuNR-Ab-HNTs hybrids or free AuNRs were irradiated with an 808 nm laser at 3–4 W cm−2, and the resulting photothermal antibacterial activity was measured via plate count. The irradiated AuNR-Ab-HNTs hybrids exerted an 8-fold higher antibacterial effect compared to free AuNR under 3.5 W cm−2; whereas the latter induced a 6 °C-higher temperature elevation. No significant antibacterial activity was observed for the AuNR-Ab-HNTs hybrid against non-target bacteria species (Serratia marcescens and Staphylococcus epidermidis). These findings are ascribed to the localization of the photothermal ablation due to the binding of the antibody-functionalized clay to its target bacteria, as supported through TEM imaging. In the future, the HNTs-based selective carriers presented herein could be tailored with other antibacterial nanoparticles or against another microorganism via the facile adjustment of the immobilized antibody.

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Apoptosis-like bacterial death modulated by photoactive hyperthermia nanomaterials and enhanced wound disinfection application

Abstract: Photothermal therapy (PTT) is considered as one efficient therapeutic strategy for wound disinfection. However, there is a dilemma that high PTT temperature for killing bacteria (>58 °C) could pose serious...

Cited by 22 publications

References 24 publications

Porous Silicon Carrier Endowed with Photothermal and Therapeutic Effects for Synergistic Wound Disinfection

Porous Silicon Carrier Endowed with Photothermal and Therapeutic Effects for Synergistic Wound Disinfection

Efficient nanozyme engineering for antibacterial therapy

Gold Nanorod-Incorporated Halloysite Nanotubes Functionalized with Antibody for Superior Antibacterial Photothermal Treatment

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