Apoptosis-mediated in vivo toxicity of hydroxylated fullerene nanoparticles in soil nematode Caenorhabditis elegans

Jeong, Yun Sook; Lee, Jaesang; Choi, Shin Sik

doi:10.1016/j.chemosphere.2011.11.054

Cited by 62 publications

(47 citation statements)

References 29 publications

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“…55 Another study demonstrated that the growth of nematodes was not affected by exposure to CeO 2 and TiO 2 NPs. 56 However, Cha et al showed that body length of nematodes exposed to fullerol NPs (diameter size: 4-40 nm) was significantly shorter than that of the nematodes in the control group. 56 Oxidative stress, which occurs when the generation of ROS leads to an imbalance between ROS and antioxidants, is involved in the toxicity mechanism of a large number of compounds.…”

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

“…56 However, Cha et al showed that body length of nematodes exposed to fullerol NPs (diameter size: 4-40 nm) was significantly shorter than that of the nematodes in the control group. 56 Oxidative stress, which occurs when the generation of ROS leads to an imbalance between ROS and antioxidants, is involved in the toxicity mechanism of a large number of compounds. 57 In the present study, we observed an increase in ROS production in a dose-dependent manner.…”

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

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Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Charão

Souto

Brucker

et al. 2015

IJN

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Caenorhabditis elegans is an alternative in vivo model that is being successfully used to assess the pharmacological and toxic effects of drugs. The exponential growth of nanotechnology requires the use of alternative in vivo models to assess the toxic effects of theses nanomaterials. The use of polymeric nanocapsules has shown promising results for drug delivery. Moreover, these formulations have not been used in cases of intoxication, such as in treatment of paraquat (PQ) poisoning. Thus, the use of drugs with properties improved by nanotechnology is a promising approach to overcome the toxic effects of PQ. This research aimed to evaluate the absorption of rhodamine B-labeled melatonin (Mel)-loaded lipid-core nanocapsules (LNC) by C. elegans , the application of this model in nanotoxicology, and the protection of Mel-LNC against PQ damage. The formulations were prepared by self-assembly and characterized by particle sizing, zeta potential, drug content, and encapsulation efficiency. The results demonstrated that the formulations had narrow size distributions. Rhodamine B-labeled Mel-LNC were orally absorbed and distributed in the worms. The toxicity assessment of LNC showed a lethal dose 50% near the highest dose tested, indicating low toxicity of the nanocapsules. Moreover, pretreatment with Mel-LNC significantly increased the survival rate, reduced the reactive oxygen species, and maintained the development in C. elegans exposed to PQ compared to those worms that were either untreated or pretreated with free Mel. These results demonstrated for the first time the uptake and distribution of Mel-LNC by a nematode, and indicate that while LNC is not toxic, Mel-LNC prevents the effects of PQ poisoning. Thus, C. elegans may be an interesting alternative model to test the nanocapsules toxicity and efficacy.

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Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

Section: Caenorhabditis Elegans As An Alternative In Vivo Modelmentioning

confidence: 99%

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Charão

Souto

Brucker

et al. 2015

IJN

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“…For survival assay, adult worms were transferred daily to new NGM agar plates, and living or dead animals were scored under a stereomicroscope (Olympus SZ51, Tokyo, Japan). The brood size was examined by counting eggs from 100 animals fed with or without carotenoids for 5 days in adulthood (Cha, Lee, & Choi, 2011). Growth was assessed by observing the body size of 5 day-old adult worms in each condition (Chen, McCloskey, Joshi, & Rothman, 2008).…”

Section: Assessment Of Survival Ratio Brood Size and Growth Of C Elmentioning

confidence: 99%

Enhanced biological activity of carotenoids stabilized by phenyl groups

et al. 2015

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“…Jednak wcześniejsze badania Cha i wsp. [41] wskazują, że fulerenol prowadzi do skrócenia długości życia, obniżenia zdolności reprodukcyjnych oraz zmniejszenia wielkości ciała w wyniku inicjowania śmierci apoptotycznej komórek nicienia przy stężeniach powyżej 10 mg/l.…”

Section: Toksyczność Fulerenoliunclassified

Fullerenols in therapy and diagnosis of cancer

Lichota¹,

Krokosz²

2016

Med Pr

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StreszczenieDo głównych przyczyn zgonów w Polsce zalicza się nowotwory złośliwe. Jednym z celów współczesnych badań biomedycznych jest zmaksymalizowanie efektów działania stosowanych strategii leczniczych. Podejmowane działania doskonalące środki terapeutyczne są ukierunkowane na ograniczenie działań niepożądanych terapii przeciwnowotworowych. Innym kierunkiem badań jest poszukiwanie substancji ochronnych, które zmniejszą toksyczność leków wobec komórek prawidłowych. Nanotechnologia umożliwia projektowanie wyspecjalizowanych nanocząstek, dzięki którym możliwe będzie skuteczniejsze leczenie chorób nowotworowych, a także ich bezpieczne diagnozowanie. Nanomateriały na bazie węgla (fulereny i ich pochodne, grafen, nanorurki wę-glowe, nanodiamenty) stanowią obszerną grupę nanocząstek, które mają potencjalne zastosowania biomedyczne zarówno w terapii, jak i diagnostyce. Celem niniejszej pracy jest omówienie właściwości biologicznych fulerenoli w kontekście ich wykorzystania w różnych strategiach terapii przeciwnowotworowych. W pracy omówiono także możliwości jednoczesnego wykorzystania nanocząstek w terapii i diagnostyce, czyli teranostyce. Obecny stan wiedzy wskazuje, że fulereny i ich hydrofilowe pochodne, w szczególności fulerenole, wykazują niską toksyczność lub jej brak. Poprzez swoje właściwości antyoksydacyjne, a także regulujące ekspresję genów zaangażowanych w proces apoptozy i angiogenezy oraz stymulowanie odpowiedzi immunologicznej, mogą przyczyniać się do hamowania rozrostu guza i ochrony komórek prawidłowych. Gadolin zamknięty w klatce fulerenu jest mniej toksyczny jako środek cieniujący w rezonansie magnetycznym i może jednocześnie hamować rozwój nowotworu, co jest obiecującym wynikiem dla teranostyki. Med. Pr. 2016;67(6):817-831 Słowa kluczowe: nanotechnologia, fuleren, fulerenol, terapia przeciwnowotworowa, obrazowanie molekularne, teranostyka AbstractMalignant tumors are one of the main causes of death in Poland. One of the objectives of contemporary biomedical research is to maximize the effects of therapeutic strategies. The actions undertaken to improve therapeutic agents are aimed at reducing the side effects of cancer treatments. Another direction of investigations is the search for protective substances that reduce the toxicity of the drug to normal cells. Carbon-based nanomaterials (fullerenes and their derivatives, graphene, carbon nanotubes, nanodiamonds) are a broad class of nanoparticles that have potential biomedical applications in both therapy and diagnostics. The aim of this paper is to review biological properties of fullerenols in the context of their use in various strategies of cancer treatments. The authors also discuss the possibility of simultaneous use of nanoparticles in therapy and diagnosis, that is, in theranostics. Current knowledge indicates that fullerenes and their hydrophilic derivatives, especially fullerenols, show low or no toxicity. They may contribute to the inhibition of tumor growth and protection of normal cells through their antioxidant properties, as well as t...

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Apoptosis-mediated in vivo toxicity of hydroxylated fullerene nanoparticles in soil nematode Caenorhabditis elegans

Cited by 62 publications

References 29 publications

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage

Enhanced biological activity of carotenoids stabilized by phenyl groups

Fullerenols in therapy and diagnosis of cancer

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