Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease

Rybickova, Ivana; Vesela, Viera; Fales, Ivan; Skalická, Pavlína; Jirsová, Kateřina

doi:10.5507/bp.2016.001

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…Second, ECs may directly interact with neighboring B or T lymphocytes and undergo apoptosis, again through Fas/FasL interactions. Third, activated cytotoxic B and T lymphocytes may release autoantibodies and cytokines, with ensuing activation of the caspase cascade [ 6 , 8 , 14 – 19 , 22 ]. Our data reinforce the suggestion that B lymphocytes induce EC apoptosis; however, this is the first time that the role of a leptin/OB-R-dependent B lymphocyte apoptotic pathway has been established.…”

Section: Discussionmentioning

confidence: 99%

Leptin/OB-R pathway promotes IL-4 secretion from B lymphocytes and induces salivary gland epithelial cell apoptosis in Sjögren's syndrome

Xie

et al. 2017

Oncotarget

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Sjögren's syndrome (SjS) is a chronic autoimmune epithelitis in which cell apoptosis promotes the formation of inflammatory lesions. We used immunohistochemistry and TUNEL to assay B cell infiltration and apoptosis in salivary gland tissue from 16-week-old NOD/LtJ mice with SjS. In co-cultures of primary salivary glandepithelial cells (SGECs) and spleen B cells, we assessed SGEC viability and apoptosis using CCK8 assays and flow cytometry. ELISAs were employed to assess cytokine levels in culture medium. Leptin protein, leptin receptor (OB-R), pro- and anti-apoptotic proteins, and Jak2/Stat3/ERK signaling molecules were analyzed using western blotting. B cell infiltration and salivary gland apoptosis were increased in salivary tissue from mice with SjS. Leptin treatment had no effect on cell viability or apoptosis among B cells and primary SGECs. B cell and SGEC co-culture systems showed that leptin increased apoptosis induced by B lymphocytes, reduced SGEC cell viability, and promoted IL-4 secretion from B cells. This suggests Leptin/OB-R signaling stimulates B cells-induced SGEC apoptosis via IL-4 secretion and OB-R-Jak2-Stat3 activation.

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Section: Discussionmentioning

confidence: 99%

Leptin/OB-R pathway promotes IL-4 secretion from B lymphocytes and induces salivary gland epithelial cell apoptosis in Sjögren's syndrome

Xie

et al. 2017

Oncotarget

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“…Less reproducible outcomes in numerous clinical and preclinical research have been caused by the lack of characterization of AS and PRP and therefore standardization of the AS and PRP preparation and storage techniques should be provided. 14,15,27,28,39 Furthermore, future in vivo studies are necessary because in vitro testing alone does not provide a solid foundation for the use of AS and PRP in corneal endothelial failure.…”

Section: Discussionmentioning

confidence: 99%

Effects of Autologous Serum and Platelet-Rich Plasma on Human Corneal Endothelial Cell Regeneration: A Comparative Study

Kilic-Toprak,

Cort-Donmez,

Toprak

2023

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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Objectives: To investigate the effects of autologous serum (AS) and platelet-rich plasma (PRP) on human corneal endothelial cell (HCEC) proliferation and apoptosis in comparison to Y-27632 as the commonly studied Rho-associated kinase (ROCK) inhibitor. Methods: The human corneal endothelial primary cell line was used for this study. As the treatment groups, HCECs were incubated with AS, PRP, and Y-27632, whereas the control group received no treatment. Cell proliferation (measured by 5-bromo-2′-deoxyuridine [BrdU] incorporation) and apoptosis (based on the caspase-3 level) were compared between the control, Y-27632, AS, and PRP groups. Results: In the Y-27632, AS, and PRP groups, the ratios of BrdU-incorporated cells were significantly higher (115±0.2%, 125±0.2%, 122±0.4% at 24 hr, and 138±2.4%, 160±0.2%, 142±0.2% at 48 hr, respectively) than in the control group (100±18.4% at 24 hr, 100±1.1% at 48 hr) (P<0.05 for all). Furthermore, AS provided a higher HCEC proliferation ratio compared with the Y-27632 group at 24 and 48 hr (P<0.05 for all). Caspase-3 was significantly lower in the AS group (60.3±3.3%) than in the control (100±2.3%), Y-27632 (101.9±5.2%), and PRP (101±6.8%) groups (P<0.05 for all). Conclusions: The results of this study demonstrated for the first time that AS and PRP promoted HCEC proliferation and AS significantly decreased apoptosis in HCECs. A superior effect on HCEC proliferation was also observed with AS compared with Y-27632. Future “autologous” regenerative therapeutic options for corneal endothelial failure may involve the utilization of AS and PRP owing to their accessibility, simplicity in preparation, immunologic compatibility, and donor-free nature.

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“…Patients administered the autologous serum eye‐drops for three months and each administration took place approximately 15 minutes after the application of artificial tears. The number of autologous serum applications per day was dependent on the number of prescribed artificial tear applications, a minimum of six times and a maximum of 12 times as described previously and the patients were instructed not to change the frequency of the treatment during the study, even if they experienced subjective improvement of their symptoms …”

Section: Methodsmentioning

confidence: 99%

“…An improvement of dry eye disease has been demonstrated after autologous serum eye‐drop treatment by standard clinical examination . A decrease in the number of apoptotic superficial epithelial cells has been demonstrated recently after autologous serum application in patients with dry eye disease of graft‐versus‐host disease (GVHD) aetiology …”

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confidence: 99%

Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

Mahelková

Jirsová

Stangova

et al. 2017

Clinical and Experimental Optometry

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The results demonstrate the ability of corneal confocal microscopy to evaluate an improvement in the basal epithelial cell layer of the cornea after autologous serum treatment in patients with dry eye disease. More studies with longer follow-up periods are needed to elucidate the suitability of corneal confocal microscopy to follow the effect of autologous serum treatment on nerve fibres or other corneal layers in dry eye disease patients.

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Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease

Cited by 12 publications

References 32 publications

Leptin/OB-R pathway promotes IL-4 secretion from B lymphocytes and induces salivary gland epithelial cell apoptosis in Sjögren's syndrome

Leptin/OB-R pathway promotes IL-4 secretion from B lymphocytes and induces salivary gland epithelial cell apoptosis in Sjögren's syndrome

Effects of Autologous Serum and Platelet-Rich Plasma on Human Corneal Endothelial Cell Regeneration: A Comparative Study

Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

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