Apoptosis of human ovarian tissue is not increased by either vitrification or rapid cooling

Salehnia, Mojdeh; Sheikhi, Maryam; Pourbeiranvand, Shahram; Lundqvist, Monalill

doi:10.1016/j.rbmo.2012.07.021

Cited by 28 publications

(26 citation statements)

References 205 publications

(265 reference statements)

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“…Furthermore, the few which have investigated the effects of cryoprotectant exposure alone on follicular health, without subsequent vitrification, have looked at the follicular health and morphology either immediately after exposure to CPAs [22] or after a short incubation period (15 min [23]; 2 h [24,25]). Because cryopreservation acts by suspending cellular metabolism, the tissue must be cultured long enough for the effects of CPA exposure on cell metabolism and function to be evident.…”

Section: Discussionmentioning

confidence: 99%

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Mouttham

Fortune

Comizzoli

2015

J Assist Reprod Genet

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Purpose The objective of this study is to characterize the impact of exposure to cryoprotectants followed by vitrification on primordial follicle survival and activation using a fetal bovine model. Methods In the first study, fetal bovine cortical pieces were exposed to cryoprotectants with or without sucrose and cultured up to 7 days in the presence or absence of insulin. In the second study, cortical pieces were exposed to cryoprotectants with or without sucrose, vitrified, and cultured up to 7 days after warming in the presence or absence of insulin. Viability and morphology of follicles, as well as proliferation and/or DNA repair in ovarian tissue were analyzed.Results When compared to non-exposed controls, normal follicular morphology was affected in groups exposed to cryoprotectants only immediately post-exposure and after 1 day of culture, but improved by day 3 and did not significantly differ by day 7. Similarly, normal follicular morphology was compromised in vitrified groups after warming and on day 1 compared to controls, but improved by days 3 and 7. Proliferation and/or DNA repair in ovarian tissue was not affected by vitrification in this model. Cryoprotectant exposure and vitrification of ovarian tissue did not impair the activation of primordial follicles in response to insulin, although activation was delayed relative to non-exposed controls. Interestingly, sucrose had no noticeable protective effect. Conclusion Vitrified fetal bovine ovarian tissue has the intrinsic capacity to mitigate the immediate damage to primordial follicles' morphology and retains the capacity to activate. These findings provide a basis for a successful cryopreservation protocol for ovarian cortical tissue in other species including humans.

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Section: Discussionmentioning

confidence: 99%

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Mouttham

Fortune

Comizzoli

2015

J Assist Reprod Genet

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“…Li et al (2007) found no significant differences in the proportion of morphologically normal primordial and primary follicles after in vitro culture (14 days). Ultra-strutural chromatin features of oocytes and follicular cells were normal after vitrified-thawed ovarian tissue and in vitro culture for one day, which was similar to the fresh tissue controls (Salehnia et al, 2012). These authors also demonstrated that the use of a vitrification solution based on the content of EG or a mixture of EG and DMSO did not affect primordial or primary follicle morphology after one day of tissue culture.…”

Section: Advances In the In Vitro Culture Of Vitrified Women Ovarian mentioning

confidence: 59%

“…Follicle morphology has been evaluated after vitrified-thawed ovarian tissue in vitro culture for short or long periods (between one and 21 days) (Salehnia et al, 2012;Isachenko et al, 2003) and has been reported that it is possible to preserve morphology similar to that of fresh tissue (Salehnia et al, 2012;Isachenko et al, 2008;Lee et al, 2000;Isachenko et al, 2003) similar to slow freezing tissue (Huang et al, 2008), or even better than slow freezing tissue (Keros et al, 2009). Li et al (2007) found no significant differences in the proportion of morphologically normal primordial and primary follicles after in vitro culture (14 days).…”

Section: Advances In the In Vitro Culture Of Vitrified Women Ovarian mentioning

confidence: 99%

“…Other characteristics have also been evaluated in in vitro cultured ovarian tissues after vitrification such as apoptosis, DNA fragmentation (Salehnia et al, 2012) and oocyte maturation (Zhang et al, 1995). Apoptosis was similar when compared to slow freezing tissue after ten days of in vitro culture (Huang et al, 2008).…”

Section: Advances In the In Vitro Culture Of Vitrified Women Ovarian mentioning

confidence: 99%

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Ewe Ovarian Tissue Vitrification: A Model for the Study of Fertility Preservation in Women

Lunardi¹,

Bass²,

Bernuci³

et al. 2015

JBRA Assisted Reproduction

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“…The RT conditions consisted of 10 minutes of annealing at 25 C, 120 minutes of cDNA synthesis at 37 C, and 5 minutes of inactivation at 85 C. The expression of the genes encoding c-Myc, caspase 3, AMH, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was quantified by real-time PCR using a commercial kit (Power SYBR Green PCR Master Mix) and the StepOne Real-Time PCR System (Applied Biosystems Pty Ltd). The primers were designed using Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi) based on the sequences from the GenBank database.…”

Section: Reverse Transcription and Real-time Quantitative Polymerase mentioning

confidence: 99%

Antiapoptotic Agent Sphingosine-1-Phosphate Protects Vitrified Murine Ovarian Grafts

et al. 2014

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Significant follicle loss from frozen ovarian grafts is unavoidable. The authors evaluated the protective effects of the antiapoptotic agent sphingosine-1-phosphate (S1P) on vitrified ovarian grafts. Three-week-old sexually immature female FVB mice were divided into 4 groups, fresh, control without S1P, 0.5 mmol/L S1P, and 2 mmol/L S1P. The ovaries were pretreated with S1P for 1 hour and then cryopreserved by modified vitrification. The frozen-thawed ovaries were autotransplanted under the back muscles of mice for 10 days. Expression of apoptosis-related genes encoding caspase 3 and c-Myc was analyzed in the vitrified ovaries and 10 days after transplantation using real-time quantitative polymerase chain reaction. To quantify the ovarian reserve, anti-Müllerian hormone (AMH) levels and follicles were measured in the 10-day vitrified ovarian grafts. Caspase 3 and c-Myc messenger RNA did not differ significantly in the 4 groups after vitrification but was significantly upregulated in the control group after transplantation. The AMH levels and primordial follicle pool were significantly higher in the S1P-treated groups than in the control group but lower than that in the fresh group. The S1P protects vitrified ovarian grafts from ischemic reperfusion injury rather than from vitrification-associated process.

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Apoptosis of human ovarian tissue is not increased by either vitrification or rapid cooling

Cited by 28 publications

References 205 publications

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Ewe Ovarian Tissue Vitrification: A Model for the Study of Fertility Preservation in Women

Antiapoptotic Agent Sphingosine-1-Phosphate Protects Vitrified Murine Ovarian Grafts

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