Apoptosis of thymocytes in developing sea bassDicentrarchus labrax(L.)

Abelli, Luigi; Baldassini, Maria Rosaria; Meschini, Roberta; Mastrolia, Lucia

doi:10.1006/fsim.1997.0116

Cited by 42 publications

(29 citation statements)

References 27 publications

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“…The involution of the thymus with age as seen in higher vertebrates is not a consistent feature in fish and variation exists between species. It has been observed that apoptotic processes occur throughout thymic development in teleost fish but decline with age (Abelli et al, 1998). Involution of the thymus has also been observed in post-spawning females (Nakanishi, 1986) and in general it seems that the size of the thymus varies with seasonal changes and hormonal cycles.…”

Section: Thymusmentioning

confidence: 93%

The morphology of the immune system in teleost fishes

Press

Evensen

1999

Fish & Shellfish Immunology

531

269

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Section: Thymusmentioning

confidence: 93%

The morphology of the immune system in teleost fishes

Press

Evensen

1999

Fish & Shellfish Immunology

531

269

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“…Indeed, the observed faint reactivity to both the DLT15 and TcRβ probes exhibited by thymocytes in the medullary (inner) portion of thymus suggests that: (1) a lower number of lymphocytes is dispersed in the meshwork of large reticular epithelial cells compared with the cortical portion (Romano et al 1999a, b;Abelli et al 1996), (2) a major proportion of medullary lymphocytes are still blasts and have to rearrange the TcR chains. Furthermore, apoptotic thymocytes are much less frequent in the medulla than in the cortex and C/M (Romano et al 1999b;Abelli et al 1998). Medullary lymphocytes have a significantly larger size and more abundant RNA (methyl green-pyronine staining; L. Abelli, unpublished) than do small T-cells in the cortex and C/M border .…”

Section: Thymusmentioning

confidence: 95%

Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

et al. 2007

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Real-time polymerase chain reaction (PCR) and in situ hybridization analyses were performed to investigate the occurrence and distribution of T-lymphocytes expressing TcRbeta in intestine and lymphoid tissues of the bony fish, Dicentrarchus labrax (sea bass). Immunohistochemistry with the monoclonal antibody DLT15 (pan-T-cell marker) was carried out to compare the cytology, distribution and number of T-cells and TcRbeta+ cells in the various sampled lymphoid organs. The highest TcRbeta expression was revealed by real-time PCR in the thymus, with high levels also being found in the gut. In the thymus, DLT15+ and TcRbeta+ cell populations were concentrated in the cortex and TcRbeta+ cells were notably reactive at the cortical-medullary border, suggesting a specialized role of this region in thymocyte selection. The density of DLT15+ T-cells increased from the anterior to posterior intestine, whereas TcRbeta+ lymphocytes were more numerous in the middle intestine compared with other segments. The existence, in fish thymus, of a medulla and a cortex comparable with those of mammals is revealed by this study. The concentration of TcRbeta+ cells in the sea bass midgut also strongly suggests a special role of this intestinal segment in antigen-specific cellular immunity. The large population of TcRbeta(-)/DLT15+ T-cells in the posterior gut can probably be ascribed to the TcRgammadelta phenotype fraction.

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“…Many individuals in this cell population undergo apoptosis as part of the T cell maturation process [22]. In murine models, 98% of immature thymocytes arriving in the thymus die via the apoptotic pathway [23].…”

Section: Introductionmentioning

confidence: 99%