Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

Romano, Nicla; Rossi, Francesca; Abelli, Luigi; Caccia, Elisabetta; Piergentili, Rita; Mastrolia, Lucia; Randelli, Elisa; Buonocore, Francesco

doi:10.1007/s00441-007-0429-z

Cited by 35 publications

(46 citation statements)

References 37 publications

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“…It was observed a relevant increment of density in hyperoxygenated fish compared with the NO group. Similar observations occurred previously in normal oxygenated sea bass by immune-histochemistry and flow cytometry [18,26,27]. Taking together the data of distribution of lymphocytes evidenced that hyperoxia treatment (oxygenation of 13 mg/L) induces selective increments of T-and B-cells among hematopoietic tissues.…”

Section: Discussionsupporting

confidence: 87%

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Romano

Scapigliati

Abelli

2017

Fishes

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Abstract:Intensive aquaculture systems have stimulated proper management in order to limit stressful stocking conditions and environmental impact. This should improve fish wellness and counteract adverse water characteristics such as high concentrations of carbon. The oxygen can be considered a key factor since critical conditions leading to chronic rise of blood catecholamines can depress the number and Oxygen-carrying capacity of erythrocytes. In this research, we evaluated the effects of variation in water oxygen (from 6 ± 1 to 13 ± 1 mg/L) on lymphocytes density in sea bass by using flow cytometry and immunohistochemistry. Gut, gill, and thymus T-cells were significantly enhanced in percentage in hyperoxygenated fish compared with normo-oxygenated group, while Peripheral Blood Leukocytes (PBL), head kidney, and spleen T-cells were not significantly different. Contrarily, in PBL, head kidney and spleen hyper-oxygenation treatment provoked an enhancement of B lymphocyte percentages. The distribution of positive T-cells in hyper-oxygenated fish varied in the organs and followed the relative increments/decrements observed in flow cytometry. This study remarks the sensitivity of sea bass adaptive immunocytes to oxygen concentration, evidenced by the modification of parameters. Sea bass culture under high-flow water recycling and at around 12-13 mg/L oxygen concentrations is therefore suggested to enhance the immune response capacity.

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Section: Discussionsupporting

confidence: 87%

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Romano

Scapigliati

Abelli

2017

Fishes

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“…In mammalian and fish mucosal membranes, intraepithelial leukocytes mainly consist of T cells and some macrophages (Picchietti et al 1997;Zapata & Amemiya, 2000;Huttenhuis et al 2006;Romano et al 2007). In addition, dendritic cells are able to send cytoplasmic protrusions through the epithelium towards the intestinal lumen for antigen recognition and sampling (Rescigno et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon

et al. 2008

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In addition to being the respiratory organ in fish, the gills form a barrier against the external milieu. Innate and adaptive immune system components have been detected in the gills, but lymphoid cell accumulations similar to that seen in the mammalian mucosa have not been described. The present investigations revealed cell accumulations on the caudal edge of interbranchial septum at the base of the gill filaments in the Atlantic salmon. Cytokeratin immunohistochemical staining and identification of a basal membrane and desmosome cell junctions by electron microscopy showed that the cell accumulation was located intraepithelially. Major histocompatibility complex (MHC) class II + cells were detected by immunohistochemistry, and laser capture micro-dissection and subsequentRT-PCR analysis revealed expression of T-cell receptor transcripts in the investigated tissue, suggesting the presence of T cells. The intraepithelial tissue reported here may be a suitable location for immune surveillance of gill infections, as well as a target site for new vaccine approaches and investigations of epithelial immunity. This is the first description of a lymphocyte cell aggregation within a teleostian gill epithelium network, illustrating a phylogenetically early form of leukocyte accumulations in a respiratory organ.

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“…1a). Very interestingly, it should be noted that in the intestine of sea bass the number of TRbexpressing T cells decreases from the anterior to the posterior part [20], thus revealing a differential distribution of T cell subtypes likely related to physiological activities.…”

Section: T Cell Receptorsmentioning

confidence: 98%

Transcription of T cell-related genes in teleost fish, and the European sea bass (Dicentrarchus labrax) as a model

Boschi

Randelli

Buonocore

et al. 2011

Fish & Shellfish Immunology

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In recent years the cloning of genes coding for immuno-regulatory peptides, as well as the sequencing of genomes, provided fish immunologists with a growing amount of information on nucleotide sequences. Research is now also addressed in investigating the functional immunology counterpart of nucleotide sequence transcripts in various fish species. In this respect, studies on functional immunology of T cell activities are still at their beginning, and much work is needed to investigate T cell responses in teleost fish species. In this review we summarise the current knowledge on the group of genes coding for main T cell-related peptides in fish, and the expression levels of these genes in organs and tissues. Particular attention is paid to European sea bass (Dicentrarchus labrax), a marine species in which some information on functional immunology has been obtained, and we reassume here the expression of some T cell-related genes in basal conditions. In addition, we provide original data showing that T cells purified from the intestinal mucosa of sea bass with a specific mAb, express transcripts for TRβ, TRγ, CD8α, and RAG-1, thus showing similarities with intra-epithelial leucocytes of mammals.

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Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

Cited by 35 publications

References 37 publications

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Water Oxygen Content Affects Distribution of T and B Lymphocytes in Lymphoid Tissues of Farmed Sea Bass (Dicentrarchus Labrax)

Identification and characterization of a novel intraepithelial lymphoid tissue in the gills of Atlantic salmon

Transcription of T cell-related genes in teleost fish, and the European sea bass (Dicentrarchus labrax) as a model

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