Apoptotic and Anti-Inflammatory Effects of<i> Eupatorium japonicum</i> Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Shin, Jong-Il; Jeon, Yong-Joon; Lee, Sol; Lee, Yoon Gyeong; Kim, Ji Beom; Kwon, Hak Cheol; Kim, Sung Hun; Kim, Inki; Lee, Kyung-Ho; Han, Ye

doi:10.1155/2018/1383697

Cited by 16 publications

(13 citation statements)

References 53 publications

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“…All statistical tests were based on the normal (NC) group and TNFa (NC) group, respectively (*p \ 0.05; **p \ 0.01). All the data were presented as the mean ± SD of three independent experiments and FLSs from three donor were used joint and ultimately disability (Shin et al 2018). RA-FLSs display 'tumor-like' features, including increased migration, invasion and reduced apoptosis (Nakamura et al 2018).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-21-5p are involved in apoptosis and invasion of fibroblast-like synoviocytes through PTEN/PI3K/AKT signal

et al. 2019

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The function of microRNA-21-5p (miR-21) in fibroblast-like synoviocytes in RA was still unclear. In our study, we used tumor necrosis factor alpha (TNFa) (10 ng/ml) to mimic RA-FLSs and we found that normal FLS stimulated with TNFa caused the increasing expression of miR-21, a disintegrin and metalloproteinase with thrombospondin motifs 5 and matrix metalloproteinase 3, which were in accord with RA-FLSs changes. Our data showed that miR-21 overexpression significantly increased cell invasion and decreased apoptosis in FLSs. Knockdown of miR-21 in FLSs causes the opposite result. However, miR-21 may not affect the proliferation of FLSs. Meanwhile, we showed that miR-21 activated the PI3K/ AKT signaling pathway to participate in RA by inhibiting PTEN expression. Taken together, our results suggested that miR-21 may play a positive role in RA and may be a promising new therapeutic target for RA. Keywords Fibroblast-like synoviocytes Á MicroRNA-21-5p Á Apoptosis Á Invasion Á PTEN/PI3K/ AKT signal Xin Yan and Yake Liu have contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-21-5p are involved in apoptosis and invasion of fibroblast-like synoviocytes through PTEN/PI3K/AKT signal

et al. 2019

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“…The results showed that DK-59 reduced cell viability and induced ROS production and apoptosis in MH7A cells via up-regulating ATF4 and CHOP, activating Caspase-7 and PARP, as well as increasing phosphorylation of eIF2α [120]. Similarly, the extracts of Eupatorium japonicum Thunb (EJTE, 37.5 µg/mL) also showed apoptosis inducing activities in RA-FLS via ERS-mediated apoptotic pathway, such as up-regulation of ATF4 and CHOP [121]. Another in vitro experiment by Lu et al showed that in the presence of H 2 O 2 , resveratrol (1) (50-400 µM) could induce the apoptosis in FLS with a dose-dependent manner.…”

Section: Ers Mediated Apoptotic Pathwaymentioning

confidence: 96%

Apoptosis Induction of Fibroblast-Like Synoviocytes Is an Important Molecular-Mechanism for Herbal Medicine along with its Active Components in Treating Rheumatoid Arthritis

et al. 2019

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Rheumatoid arthritis (RA) is a known chronic autoimmune disease can cause joint deformity and even loss of joint function. Fibroblast-like synoviocytes (FLS), one of the main cell types in synovial tissues of RA patients, are key effector cells in the development of RA and are considered as promising therapeutic targets for treating RA. Herbal medicines are precious resources for finding novel agents for treating various diseases including RA. It is reported that induction of apoptosis in FLS is an important mechanism for the herbal medicines to treat RA. Consequently, this paper reviewed the current available references on pro-apoptotic effects of herbal medicines on FLS and summarized the related possible signal pathways. Taken together, the main related signal pathways are concluded as death receptors mediated apoptotic pathway, mitochondrial dependent apoptotic pathway, NF-κB mediated apoptotic pathways, mitogen-activated protein kinase (MAPK) mediated apoptotic pathway, endoplasmic reticulum stress (ERS) mediated apoptotic pathway, PI3K-Akt mediated apoptotic pathway, and other reported pathways such as janus kinase/signal transducers and activators of transcription (JAK-STAT) signal pathway. Understanding the apoptosis induction pathways in FLS of these herbal medicines will not only help clear molecular mechanisms of herbal medicines for treating RA but also be beneficial for finding novel candidate therapeutic drugs from natural herbal medicines. Thus, we expect the present review will highlight the importance of herbal medicines and its components for treating RA via induction of apoptosis in FLS, and provide some directions for the future development of these mentioned herbal medicines as anti-RA drugs in clinical.

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“…According to reactivity towards the earlier mentioned DHPAs, the MAb 4B8‐based icELISA detected an amount of cross‐reactive compounds in samples of Senecio vulgaris and E. japonicum that previously had no MCT content reported. Positive results were obtained due to its CR against senecionine and retrorsine in Senecio vulgaris or MCT‐related pyrrolizidine alkaloids in E. japonicum . The high contents of these compounds in Senecio vulgaris and E. japonicum produced positive results in the Mab 4B8‐based icELISA.…”

Section: Resultsmentioning

confidence: 99%

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Yusakul

Sakamoto

Chanpokapaiboon

et al. 2019

Phytochemical Analysis

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Introduction Monocrotaline (MCT), which is classified as a 1,2‐dehydropyrrolizidine alkaloid (DHPA), is a toxic compound that is mainly produced by Crotalaria spp. MCT contamination in cereals and herbs leads to hepatitis, gastroenteritis, pulmonary vasculitis and hypertension, and different types of cancer. The current analytical methods for MCT are complicated and expensive using liquid chromatography equipped with mass spectrometry detection. Objective The aim of this study was to develop a simple and sensitive preincubation format for an immunochromatographic assay (PI‐ICA) for MCT detection. Methodology We conducted the PI‐ICA via incubation of an MCT‐containing sample with an anti‐MCT monoclonal antibody conjugated with colloidal gold before strip dipping. We compared the PI‐ICA detection sensitivity with that of the conventional ICA (Conv‐ICA) format. Results The PI‐ICA was sensitive with a limit of detection (LOD) of 0.61 ng/mL, which is a 16‐fold improvement over the Conv‐ICA format. These results indicated that the PI‐ICA method exhibits high binding specificity for MCT and low cross‐reactivity towards retronecine, retrorsine, senecionine and heliotrine. Sample solutions from plants containing MCT and related DHPAs produced positive results via PI‐ICA analysis. Conclusions The proposed PI‐ICA system provides a highly sensitive method compared to Conv‐ICA. In addition, the developed PI‐ICA method is simple and highly effective for detecting MCT contamination.

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Apoptotic and Anti-Inflammatory Effects of Eupatorium japonicum Thunb. in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

Cited by 16 publications

References 53 publications

MicroRNA-21-5p are involved in apoptosis and invasion of fibroblast-like synoviocytes through PTEN/PI3K/AKT signal

MicroRNA-21-5p are involved in apoptosis and invasion of fibroblast-like synoviocytes through PTEN/PI3K/AKT signal

Apoptosis Induction of Fibroblast-Like Synoviocytes Is an Important Molecular-Mechanism for Herbal Medicine along with its Active Components in Treating Rheumatoid Arthritis

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

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