Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Lei, Bin; Zhou, Xuming; Lv, Daojun; Wan, Bo; Wu, Haoyue; Zhong, Lintao; Shu, Fangpeng; Mao, Xiangming

doi:10.4103/1008-682x.169563

Cited by 17 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caspase 7 was major mainly in spermatogenic cells and have apoptotic function in spermatogenic dysfunction. 27 In the current study, we found that LBP could reverse DDP-induced caspase 3, caspase 7, and caspase 12 upregulation. In addition, the formation of autophagic vacuoles was detected using immunohistochemistry and MDC staining.…”

Section: Discussionsupporting

confidence: 51%

<em>Lycium barbarum</em> polysaccharide prevents cisplatin-induced MLTC-1 cell apoptosis and autophagy via regulating endoplasmic reticulum stress pathway

Yang¹,

Wei²,

Liao³

et al. 2018

DDDT

View full text Add to dashboard Cite

BackgroundLycium barbarum polysaccharide (LBP) has been reported to contribute to the recovery of male hypogonadism and infertility.AimThe aim of current study was to investigate the underlying mechanisms of LBP on male infertility recovery.MethodsRecently, it is reported that cell apoptosis mediated by endoplasmic reticulum stress (ERS) was distinguished from that mediated by death reporters and mitochondria pathway, which could induce cell apoptosis independently. The possible signaling mechanisms were investigated using diversified molecular biology techniques, such as flow cytometry, western blotting, and immunofluorescence.ResultsIn this study, we found that LBP protected Leydig MLTC-1 cells against cisplatin (DDP) by regulating ERS-mediated signal pathway, which was evidenced by downregulation of phosphorylation PERK, phosphorylation of eukaryotic translation-initiation factor 2α and activating transcription factor 4. Meanwhile, LBP decreased DDP-induced MLTC-1 cell apoptosis via reducing ERS apoptosis-relative proteins caspase 3, caspase 7, and caspase 12. In addition, the result of monodansylcadaverine staining indicated that LBP significantly inhibited DDP-induced autophagosome formation in MLTC-1 cells. Moreover, immunofluorescences and Western blot assays demonstrated that LBP reversed DDP-induced LC3II and Atg5 upregulation in MLTC-1 cells. Finally, the data of enzyme-linked immunosorbent assay showed that LBP markedly recovered MLTC-1 cells testosterone level even in the presence of DDP.ConclusionThus, we suggest that LBP protected MLTC-1 cells against DDP via regulation of ERS-mediated apoptosis and autophagy.

show abstract

Section: Discussionsupporting

confidence: 51%

<em>Lycium barbarum</em> polysaccharide prevents cisplatin-induced MLTC-1 cell apoptosis and autophagy via regulating endoplasmic reticulum stress pathway

Yang¹,

Wei²,

Liao³

et al. 2018

DDDT

View full text Add to dashboard Cite

show abstract

“…Such activation has been previously reported in lung cancer-derived cell lines [55]. Interestingly, recent studies demonstrated that caspases can exert both apoptotic and non-apoptotic activities, and their activation does not exclusively lead to cell death [56][57][58]. Indeed, caspases are involved in regeneration, wound healing, proliferation, and in cell motility [59,60].…”

Section: Discussionmentioning

confidence: 55%

Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells

et al. 2020

View full text Add to dashboard Cite

Retinal pigment epithelial cells are crucial for retina maintenance, making their cytoprotection an excellent way to prevent or slow down retinal degeneration. In addition, oxidative stress, inflammation, apoptosis, neovascularization, and/or autophagy are key pathways involved in degenerative mechanisms. Therefore, here we studied the effects of curcumin, lutein, and/or resveratrol on human retinal pigment epithelial cells (ARPE-19). Cells were incubated with individual or combined agent(s) before induction of (a) H2O2-induced oxidative stress, (b) staurosporin-induced apoptosis, (c) CoCl2-induced hypoxia, or (d) a LED-autophagy perturbator. Metabolic activity, cellular survival, caspase 3/7 activity (casp3/7), cell morphology, VEGF levels, and autophagy process were assessed. H2O2 provoked a reduction in cell survival, whereas curcumin reduced metabolic activity which was not associated with cell death. Cell death induced by H2O2 was significantly reduced after pre-treatment with curcumin and lutein, but not resveratrol. Staurosporin increased caspase-3/7 activity (689%) and decreased cell survival by 32%. Curcumin or lutein protected cells from death induced by staurosporin. Curcumin, lutein, and resveratrol were ineffective on the increase of caspase 3/7 induced by staurosporin. Pre-treatment with curcumin or lutein prevented LED-induced blockage of autophagy flux. Basal-VEGF release was significantly reduced by lutein. Therefore, lutein and curcumin showed beneficial protective effects on human-derived retinal cells against several insults.

show abstract

“…The reaction conditions were 95°C for 10 min, followed by 95°C for 10 sec for 40 cycles and 60°C for 60 sec. The relative mRNA expression level of each sample compared with GAPDH expression was derived using the 2 −ΔΔCq method ( 18 , 19 ).…”

Section: Methodsmentioning

confidence: 99%

Downregulation of lncRNA PVT1 expression inhibits proliferation and migration by regulating p38 expression in prostate cancer

Wan

et al. 2018

Oncol Lett

Self Cite

View full text Add to dashboard Cite

Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) has been reported to be overexpressed in prostate cancer cells and associated with tumorigenesis in various types of cancer. However, the biological function of lncRNA PVT1 remains largely unknown. The aim of the present study was to investigate the effect of lncRNA PVT1 expression on the proliferation and migration of prostate cancer cells. Stably transfected prostate cancer cells with downregulated expression of lncRNA PVT1 were constructed by an efficient siRNA fragment, followed by confirmation by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Proliferation was assessed using CCK-8, colony formation and xenograft assays, and cell migration was evaluated using a wound healing assay. The PathScan® Intracellular Signaling Array kit was utilized to explore the underlying molecular mechanisms of lncRNA PVT1 expression in prostate cancer cells. RT-qPCR results confirmed that the lncRNA PVT1 expression level was successfully knocked down in prostate cancer cells. When lncRNA PVT1 expression was downregulated in prostate cancer cells, proliferation and migration were significantly inhibited, compared with the control lncRNA PVT1 group. Furthermore, PVT1 knockdown decreased the phosphorylation of p38 in DU145 cells. Therefore, the present study demonstrated that lncRNA PVT1 downregulation inhibits the proliferation and migration of prostate cancer cells, and is associated with p38 phosphorylation.

show abstract

Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

Cited by 17 publications

References 26 publications

<em>Lycium barbarum</em> polysaccharide prevents cisplatin-induced MLTC-1 cell apoptosis and autophagy via regulating endoplasmic reticulum stress pathway

<em>Lycium barbarum</em> polysaccharide prevents cisplatin-induced MLTC-1 cell apoptosis and autophagy via regulating endoplasmic reticulum stress pathway

Cytoprotective Effects of Natural Highly Bio-Available Vegetable Derivatives on Human-Derived Retinal Cells

Downregulation of lncRNA PVT1 expression inhibits proliferation and migration by regulating p38 expression in prostate cancer

Contact Info

Product

Resources

About