Apoptotic Effect of Organophosphorus Insecticide Diazinon on Rat Ovary and Protective Effect of Vitamin E

Abstract

Background: Diazinon (DZN) is an organophosphate insecticide widely utilized in agriculture all over the world and causes many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on apoptosis of ovarian follicles in adult rats and to assess the protective role of vit E. Methods: Thirty adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group 1 (DZN+olive oil, 60 mg/kg), experimental group 2 (vit E, 200 mg/kg), and experimental group 3 (DZN+vit E, the same dosage). All drugs were injected intraperitoneally, except vit E administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure apoptosis of ovarian follicles. Results: The number apoptotic cells experimental group 1 increased significantly in contrast control group in secondary and graffian follicles (P< 0.001). Administration vit E plus DZN, significantly reduced apoptotic cells compared to DZN group (P< 0.001). Conclusion: DZN-induced apoptosis in secondary and graffian follicles and vit E inhibited apoptosis induced by DZN. Vit E might have a protective effect on DZN-induced ovarian toxicity.

Protective Potential of Vitamin C and E against Organophosphate Toxicity: Current Status and Perspective

“…Exposure to chlorfenvinphos, another OP has also been linked to generation of ROS and lipid peroxides in rat liver 41 . Moreover, OP like Diazinon induces apoptosis of secondary and graffian follicles in rat ovary 42 . Sivaperumal and Sankar 43 documented that exposure to methyl parathion enhances activities of endogenous antioxidants (including Catalase, Glutathione-s-Transferase, SOD, and MDA) by several folds in fish model.…”

“…Probably the antioxidant property along with acetyl cholinesterase restoration ability plays significant role behind the protective/prophylactic/ameliorative potential of vitamin E. This can be further validated through the fact that supplementation with vitamin E successfully ameliorated CPF-induced haematological and biochemical alterations 74,75 . Vitamin E has been demonstrated to reduce Diazinon induced apoptosis in secondary and graafian follicles in rat ovary 42 . John et al 76 demonstrated that vitamin E has the potential to ameliorate dimethoate and malathion-induced OS in rat erythrocytes.…”