Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line

Çinçin, Zeynep Birsu; Unlu, Miray; Kıran, Bayram; Bireller, Elif Sinem; Baran, Yusuf; Çakmakoğlu, Bedia

doi:10.1007/s12253-014-9825-3

Cited by 36 publications

(24 citation statements)

References 22 publications

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“…Thus, optimizing an efficient A. rhizogenes-mediated hairy root culture system could be a promising approach to supply flavonoids (quercetin) from radish biomass. Quercetin has gained a lot of attention as powerful antioxidant and antifibrotic compound with cytoprotective effects (Cincin et al 2014). In medical science, it has been demonstrated that quercetin, in a dose of 150 mg day −1 , downregulates from the systolic pressure (Egert et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

et al. 2018

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An efficient protocol for hairy root induction in radish was established by optimizing several parameters that affect the efficiency of -mediated transformations. Explants wounded using sterile hypodermic needle, infected with suspension (0.6 OD) for 10 min and co-cultivated in 1/2 MS medium containing acetosyringone (100 µM) for 2 days displayed maximum percentage of hairy root induction using MTCC 2364 (77.6%) and MTCC 532 (67.6%). On further experiments with MTCC 2364 initiated hairy roots, maximum biomass accumulation (fresh weight = 9.50 g; dry weight = 1.48 g) was achieved in liquid 1/2 MS medium supplemented with 87.6 mM sucrose after 40 days of culture. Transgenic state of hairy roots of MTCC 2364 was confirmed by polymerase chain reaction using and-specific primers. The MTCC 2364-induced hairy roots produced higher amount of phenolic (33.0 mg g), flavonoid (48.0 mg g), and quercetin (114.8 mg g) content compared to auxin-induced roots of non-transformed radish. Furthermore, the results of ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl assay confirmed that the antioxidant activity of MTCC 2364 root extracts was improved when compared to auxin-induced roots of non-transformed radish. The present study offers a new insight in radish for production of phenolics and flavonoids (quercetin) using -mediated hairy root induction.

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Section: Introductionmentioning

confidence: 99%

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

et al. 2018

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“…The literature reports that compound 1 (diglycosylated flavonoid) showed antiallergic actions (Ichikawa, Ogura, Lijima, 1986), high antioxidant potential (Nascimento et al, 2013) and antileishmanial activities (Muzitano et al, 2006a). Moreover, quercetrin (2) protected skin from UVB-induced inflammation (Yin et al, 2013), displayed antioxidant (Aderogba, Okoh, Idowu, 2005;Tai et al, 2014) and antileishmanial activities (Muzitano et al, 2006b), showed antiproliferative and apoptotic effects in colon cancer cells (Cincin et al, 2015), demonstrated gastroprotective actions (Mota et al, 2009) and showed intestinal anti-inflammatory effects (Camuesco et al, 2004). Júnior et al, (2014) reported that quercitrin was present in Croton campestris extracts at 2.34% and might be involved in gastroprotective activities against ethanol, acidified ethanol-, and indomethacin-induced gastric lesions.…”

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confidence: 99%

Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.

Sobreira

Hernandes

Vetore-Neto

et al. 2017

Braz. J. Pharm. Sci.

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Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE 50 =41.91 µg/mL) by DPPH in comparison with Trolox ® and 11.33 mmol Trolox ® equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.Uniterms: Gastric ulcer/study/rats. Gastric ulcer/treatment. Kalanchoe pinnata/chromatographic profile. Kalanchoe pinnata/gastroprotective activity. Quercetrin. Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside. INTRODUCTIONFor the past two centuries, peptic ulcers have caused high morbidity and mortality (Malfertheiner, Chan, Mccoll, 2009). This disease is a serious and prevalent pathology (Calam, Baron, 2001). A peptic ulcer is a chronic lesion, usually single, that can be located in the gastric or duodenal mucosae (Kumar, Abbas, Fausto, 2005). In the UK, the incidence of this disease was 12% higher in men than in women (Araujo, Borini, Guimarães, 2014). A review article (January 1980 to February 2009 showed that the incidence rate of uncomplicated peptic ulcers was approximately one case per 1000 people per year in the general population; the incidence rate for complicated peptic ulcers that included bleeding and perforation was approximately 0.7 cases per 1000 people per year (Lin, Rodrígues, Hernándes-Diaz, 2011).The pathophysiology of peptic ulcers suggests that this disease is caused by an imbalance between defensive factors (mucus and bicarbonate secretion, mucosal barrier, blood flow, and prostaglandins) and destructive factors (reactive oxygen species and acid and pepsin secretion), resulting in the acute inflammation of the gastrointestinal mucosae (Laine, Takeuchi, Tarnawski, 2008). Several stimuli serve as offensive factors, including Helicobacter pylori, the use of nonsteroidal anti-inflammatory drugs (NSAIDs), stress, alcohol and cigarette smoking (Malfertheiner, Chan, Mccoll, 2009;Malnick et al., 2014). The current medical treatment for peptic ulcers is generally based on proton pump inhibitors and histamine H 2 -receptor blockers (Jain et al., 2007). However, the long-term use of these therapeutic agents can produce adverse effects, such as hypergastrinemia and hyperplasia in the enterochromaffinlike (ECL) cells, the risk of pneumonia, diarrhea and the disturbance of ...

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“…Also flavonoids present in fruit extracts as catechin, epicatechin, and rutin were able to inhibit MDA-MB 231 and HCT116 proliferation at concentrations higher than 100 μM (Hayes et al, 2006 ; Li et al, 2009 ). Quercitrin has antiproliferative and apoptotic effect on colon cancer cells (Cincin et al, 2015 ). Given the cytotoxic properties reported in literature for xanthones (Bennet and Lee, 1989 ; Vieira and Kijjoa, 2005 ; Demirkiran, 2007 ), 1,2,3,5-tetrahydroxyxanthone may also contribute to the final effect observed on tumor cells.…”

Section: Resultsmentioning

confidence: 99%

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

et al. 2016

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Hypericum androsaemum, also known as Tutsan, is a small evergreen shrub common in the Mediterranean basin where it is traditionally used as diuretic and hepatoprotective herbal drug. This plant possesses the peculiarity to produce fleshy and berry-like fruits that ripen from red to shiny black. In the present work, the chemical constituents of methanolic extracts and infusions of red and black fruits were analyzed by HPLC, and correlated with their antioxidant properties which were evaluated by the DPPH, β-Carotene/linoleic acid, and hypochlorous acid tests. In addition, the red pigment of the fruit was isolated by column chromatography and structurally elucidated by NMR. Results showed that H. androsaemum fruits contain high amounts of shikimic and chlorogenic acids, while their color was given by a tetraoxygenated-type xanthone, reported for the first time in Hypericum species. The red berries infusion gave the highest content of total phenolic compounds, DPPH, and hypochlorous acid scavenging activity, and β-carotene bleaching. Cytotoxicity of the berries extracts on three human tumor cell lines (malignant melanoma, breast adenocarcinoma, and colon carcinoma) was evaluated by MTT assay, and relevant inhibition on colon carcinoma cells (IC50 value of 8.4 μg/mL) was found. Finally, the effects of red berries extract on the immune system were evaluated by peripheral blood mononuclear cell (PBMC) proliferation assay that revealed a strong stimulation on lymphocytes at low doses (0.4–6 μg/mL).

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Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line

Cited by 36 publications

References 22 publications

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids

Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

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