In-Ga-Zn-O (IGZO) nanometer thin-film transistors (TFTs) are promising candidates for liquid crystal display (LCD) drivers and human body sensors. It is critically important to study the temperature dependence of IGZO TFTs on electrical properties. However, the mechanism of the enhanced IGZO TFT function at different temperatures has not been fully determined. Here, a single transistor was used to act as a temperature sensor to save the space, and transfer curves shifting positively were found for the first time, different from conventional temperature-dependent behaviors. This behavior suggests at least two mechanisms that dominate and are responsible for the different shifts. According to the Arrhenius law, the formula between temperature (T) and threshold voltage (V TH ) was modified. Besides, two different values of activation energy (E a ) on different temperature ranges indicate that there are two main mechanisms. For further verification, different experimental approaches were conducted to study the temperature effects, including subgap density of states (DOS), X-ray photoelectron spectroscopy (XPS), and simulation experiments. This mechanism, shown here for the first time, might better the understanding of TFTs and, thus, further their applications in medicine and beyond.