Apparently identical viruses from Gaeumannomyces graminis var. tritici and Phialophora sp. (lobed hyphopodia)

Jamil, Nusrat; Buck, K. W.

doi:10.1016/s0007-1536(84)80053-4

Cited by 3 publications

(7 citation statements)

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“…tritici, are common. Much variability has been found among viruses from different field isolates of G. graminis, including isolates from the same field (Frick & Lister, 1978); 18 viruses from nine isolates of this fungus have been classified into four groups (I to IV) based on their serological and biophysical properties McFadden et al, 1983 ;Buck, 1984;Jamil & Buck, 1984). Group I viruses had particles of 35 nm diameter with two to four dsRNA components in the range 1.94 to 1.53 kbp and a single capsid polypeptide species of mol.…”

Section: Introductionmentioning

confidence: 99%

Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Jamil¹,

Buck²,

Carlile³

1984

Journal of General Virology

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SUMMARYDouble-stranded RNA, from isolates of Gaeumannomyces graminis var. tritici in nine vegetative compatibility groups, was separated by polyacrylamide gel electrophoresis and transferred to aminophenylthioether-paper. Hybridization between these blots and cDNA probes prepared to double-stranded RNA from viruses obtained from three of the isolates, revealed several close relationships between double-stranded RNA from isolates in different vegetative compatibility groups. The implications of this finding for virus transmission in G. graminis are discussed.

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Section: Introductionmentioning

confidence: 99%

Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Jamil¹,

Buck²,

Carlile³

1984

Journal of General Virology

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“…tritici (Ggt), and have been divided into four groups based on their physical and serological properties and the number and sizes of their dsRNA segments and capsid polypeptides (Buck et al, 1981;McFadden et al, 1983;Buck, 1984). Viruses in groups I and II have a divided genome of two dsRNA segments and have been placed in the family Partitiviridae (Brown, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…wt. 84000 and 78000 (Jamil & Buck, 1984), is a possible member of the family Totiviridae (Brown, 1986). However, viruses in the Totiviridae have only a single capsid polypeptide.…”

mentioning

confidence: 99%

“…However, viruses in the Totiviridae have only a single capsid polypeptide. To determine whether the 87-1-H capsid polypeptides are unrelated or whether one could be derived-from the other we have compared them by peptide mapping and by determining their amino acid compositions.Ggt isolate 87-1 was cultured and virus 87-1-H was isolated and purifiedas described by Jamil & Buck (1984). For the separation of capsid polypeptides P 1 and P2, a virus suspension was first made 19/0 in SDS and 0.1% in 2-mercaptoethanol and heated to 100 °C for 3 min.…”

mentioning

confidence: 99%

“…Ggt isolate 87-1 was cultured and virus 87-1-H was isolated and purifiedas described by Jamil & Buck (1984). For the separation of capsid polypeptides P 1 and P2, a virus suspension was first made 19/0 in SDS and 0.1% in 2-mercaptoethanol and heated to 100 °C for 3 min.…”

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confidence: 99%

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Capsid Polypeptides in a Group III Virus from Gaeumannomyces graminis var. tritici Are Related

Jamil¹,

Buck

1986

Journal of General Virology

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SUMMARYPeptide mapping and amino acid analysis have shown that the two capsid polypeptides of a group III virus, 87-1-H, from Gaeumannomyces graminis are closely related.Isometric double-stranded RNA (dsRNA) virus particles are common in field isolates of the wheat take-all fungus, Gaeumannomyces graminis var. tritici (Ggt), and have been divided into four groups based on their physical and serological properties and the number and sizes of their dsRNA segments and capsid polypeptides (Buck et al., 1981;McFadden et al., 1983;Buck, 1984). Viruses in groups I and II have a divided genome of two dsRNA segments and have been placed in the family Partitiviridae (Brown, 1986). A recently described group III virus, designated 87-1-H, which has an undivided dsRNA genome of mol. wt. 4.2 x 106 and two capsid polypeptides, P1 and P2, mol. wt. 84000 and 78000 (Jamil & Buck, 1984), is a possible member of the family Totiviridae (Brown, 1986). However, viruses in the Totiviridae have only a single capsid polypeptide. To determine whether the 87-1-H capsid polypeptides are unrelated or whether one could be derived-from the other we have compared them by peptide mapping and by determining their amino acid compositions.Ggt isolate 87-1 was cultured and virus 87-1-H was isolated and purifiedas described by Jamil & Buck (1984). For the separation of capsid polypeptides P 1 and P2, a virus suspension was first made 19/0 in SDS and 0.1% in 2-mercaptoethanol and heated to 100 °C for 3 min. The denatured capsid polypeptides were then separated by polyacrylamide gel electrophoresis (PAGE) at 5 V/cm for 24 h using a 4% stacking gel, a 10% resolving gel and the Laemmli (1970) discontinuous buffer system. The gel was stained for 15 min with 0.1% Coomassie Brilliant Blue in 50% (v/v) methanol with 10% (v/v) acetic acid and then destained for 1 h in 5% (v/v) methanol with 10 ~o (v/v) acetic acid. A typical separation is shown in Fig. 1. Individual protein bands were excised, further purified by a second cycle of PAGE using 16~o resolving gels and then electroeluted (Leibowitz & Wang, 1984) into dialysis bags containing 0.05 M-Tris-HCI, 0-24 M-glycine, 0"1% SDS, pH 8.3. The solutions were adjusted to 0.2 M-KCI and stored at 0:°C for 15 min. The precipitated polypeptides were collected by centrifugation, washed with 0.1 M-HC1 in acetone and then with acetone, and then dried in vacuo. Analysis by SDS-PAGE with Coomassie Brilliant Blue staining showed that each polypeptide was free from detectable contamination by the other.For peptide mapping, polypeptides (5 to 10 p.g) were dissolved in 0.5 M-sodium phosphate, 0.19/0 SDS, pH 7.5 and iodinated with 12s I using Iodogen (Pierce Chemical Company, Rockford, IlL, U.S.A.) as described by Fraker & Speck (1978). Iodinated polypeptides were digested at 37 °C for 19 h either with trypsin (100 ~tg/ml) in 0.1 M-ammonium bicarbonate, or with pepsin (100 ~tg/ml) in 5 % (v/v) formic acid, then diluted fivefold with water and lyophilized. Peptides t Present address:

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Descriptions of Virus Families and Groups

Francki

Fauquet

Knudson

et al. 1991

Classification and Nomenclature of Viruses

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Alfalfa mosaic 392 Microviridae 178 Arenaviridae 284 Myoviridae 161 Baculoviridae 117 Necrovirus 316 Birnaviridae 200 Nepovirus 368 Bromovirus 382 Nodaviridae 372 Bunyaviridae 273 Orthomyxoviridae 263 Caliciviridae 300 Papovaviridae 146 Capillovirus 339 Paramyxoviridae 242 Carla virus 341 Parsnip yellow fleck 318 Carmovirus 303 Partitiviridae 208 Caulimovirus 150 Parvoviridae 167 Closterovirus 345 Pea enation mosaic 375 Commelina yellow mottle

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Apparently identical viruses from Gaeumannomyces graminis var. tritici and Phialophora sp. (lobed hyphopodia)

Cited by 3 publications

References 14 publications

Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Capsid Polypeptides in a Group III Virus from Gaeumannomyces graminis var. tritici Are Related

Descriptions of Virus Families and Groups

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