Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Jamil, Nusrat; Buck, K. W.; Carlile, M. J.

doi:10.1099/0022-1317-65-10-1741

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…2 kbp in size, similar to the ones we describe here have been observed [4,15]. Also a mycovirus with a similar dsRNA banding pattern to the one found in Fusarium culmorum here has been reported for F. graminearum [3] and some of the numerous viruses described in Gaeumannomyces graminis, have genomic dsRNA elements similar to the ones we show here [13]. Also, a 6.4 kbp dsRNA associated to hypovirulence occurs in some isolates of Rhizoctonia solani [24] In contrast Curvularia thermal tolerance virus has a genome consisting of two segments ca.…”

supporting

confidence: 89%

Mycoviruses are common among different species of endophytic fungi of grasses

2009

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A survey of mycoviruses was made in a collection of 103 isolates belonging to 53 different species of endophytic fungi of grasses. Double-stranded RNA (dsRNA) elements were detected in isolates of 12 of the species analyzed. The banding characteristics and sizes of some of the dsRNA elements suggest that they might belong to previously described mycovirus families. The observed incidence (22.6%) indicates that the presence of mycoviruses could be common among species of this group of ubiquitous fungi.

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supporting

confidence: 89%

Mycoviruses are common among different species of endophytic fungi of grasses

2009

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“…There is much variation in the properties of these viruses and they have been classified into five groups depending on the physical properties of the particle, the capsid polypeptide serology, and the numbers and sizes of the dsRNAs they contain (Buck, 1986). Similar viruses have been observed in different vegetative compatibility groups of Ggt, the other varieties of G. graminis and P. graminicola (Jamil and Buck, 1991; Jamil et al ., 1984; Liang and Chen, 1990; Liang et al ., 1989; McGinty et al ., 1981). The distribution of G. graminis viruses is complex; Ggt isolates from a single field can have different combinations of several different viruses.…”

Section: Viruses and Extrachromosomal Nucleic Acidssupporting

confidence: 75%

“…In intra‐isolate crosses, using mutants or antibiotic‐resistant transformants, the percentage of hybrid perithecia was generally around 10–25% but sometimes higher frequencies were obtained (Hornby et al ., 1998; Musker, 1994; Pilgeram and Henson, 1992). Also, numerous vegetative incompatibility groups exist within G. graminis (Asher, 1981; Jamil and Buck, 1991; Jamil et al ., 1984) and this places restrictions on the isolate combinations that can be sucessfully paired. Attempts to make forced heterokaryons by pairing isolates on selective medium produced stable heterokaryons only when mutants of the same isolate were used.…”

Section: Genetics and Transformation Of G Graminismentioning

confidence: 99%

Gaeumannomyces graminis, the take‐all fungus and its relatives

Freeman

Ward

2004

Molecular Plant Pathology

105

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SUMMARY Take‐all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take‐all, has led to a considerable degree of understanding of many aspects of the disease. However, effective and economic control of the disease remains difficult. The application of molecular techniques to study G. graminis and related fungi has resulted in some significant advances, particularly in the development of improved methods for identification and in elucidating the role of the enzyme avenacinase as a pathogenicity determinant in the closely related oat take‐all fungus (G. graminis var. avenae). Some progress in identifying other factors that may be involved in determining host range and pathogenicity has been made, despite the difficulties of performing genetic analyses and the lack of a reliable transformation system.

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“…Electrophoresis (PAGE) of dsRNA samples was carried out in ! S-mm-thick vertical 4% polyacrylamide gels (100:1 acrylamide:bis-acrylamide) in TAE buffer (40 mM tris, I mM EDT,^, adjusted to pH 8-2 with acetic acid) at 30 v or 60 v (constant voltage) for 16-IS h. as described by Jamil et al (1984). Gels were then stained in ethidium bromide (1 /tg/ml) for 20 min and examined for fluorescent bands on a UV transilluminator.…”

Section: Extraction and Analysis Of Dsrnamentioning

confidence: 99%

Transmission of double‐stranded RNA and a disease factor in Ophiostoma ulmi

1986

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A diseased isolate of Ophiostoma ulmi was found to contain 10 segments of double‐stranded RNA (dsRNA) with molecular weights ranging from 2.40 x 106 to 0.23 x 106. In contrast seven other healthy isolates in the same vegetative compatibility group contained either no dsRNA or up to four dsRNA segments. Transmission of the disease to healthy isolates by hyphal anastomosis was accompanied by‐transmission of the 10 dsRNA segments. In a genetic cross in which the diseased isolate acted as the female parent single‐ascospore progeny were healthy and either contained no dsRNA or only one segment of dsRNA. When elm trees were inoculated with diseased isolates, subsequent reisolations were healthy and retained only two to seven of the dsRNA segments or were diseased and retained all 10 dsRNA segments. Following conidiogenesis a diseased isolate gave rise to single‐conidial progeny which were either slow growing and diseased, like their parent, with all 10 dsRNA segments, or faster growing like healthy isolates. Some of the faster growing conidial isolates retained only two to seven of the dsRNA segments and were disease‐free. However a majority of the faster growing conidial isolates retained all 10 dsRN A segments and were shown to carry the disease in a latent form. The possibility that the disease of O. ulmi is conferred by specific segments of dsRNA and the potential of d‐factors for the control of Dutch elm disease are discussed.

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Sequence Relationships between Virus Double-stranded RNA from Isolates of Gaeumannomyces graminis in Different Vegetative Compatibility Groups

Cited by 14 publications

References 22 publications

Mycoviruses are common among different species of endophytic fungi of grasses

Mycoviruses are common among different species of endophytic fungi of grasses

Gaeumannomyces graminis, the take‐all fungus and its relatives

Transmission of double‐stranded RNA and a disease factor in Ophiostoma ulmi

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