Appendixes: Selective Compilation of 31P-NMR Data

Gorenstein, David G.; Shah, Dinesh O.

doi:10.1016/b978-0-08-091841-9.50023-0

Cited by 7 publications

(2 citation statements)

References 81 publications

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“…The sensitivity of any NMR analysis is partially dependent on the concentration of nuclei present (Gorenstein and Shah, 1984). Samples with low concentrations of P will require an inordinate number of scans in order to distinguish 31 P signals from noise.…”

Section: Lyopliilization Resolubilization and Titrationmentioning

confidence: 99%

Optimization of sample ph and temperature for phosphorus‐31 nuclear magnetic resonance spectroscopy of poultry manure extracts

Crouse

Sierzputowska-Gracz

Mikkelsen

2000

Communications in Soil Science and Plant Analysis

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Organic phosphorus (P) compounds can be characterized using nuclear magnetic resonance (NMR) spectroscopy provided conditions are suitable for detecting the NMR signal. The objective of the research was to optimize pH and temperature conditions for turkey manure extracts prior to analysis of organic P compounds using NMR. Samples of turkey manure were extracted with 0.25 MNaOH + 0.05 MEDTA. The extracts were lyophilized and resolubilized in distilled H 2 O before analysis on a General Electric GN500 NMR spectrometer. Initial 31 P NMR experiments were run to determine the optimal instrumental parameters for 31 P studies. Samples were titrated to seventeen pH values ranging from 4.0 to 13.2. Samples adjusted to pH 10.0 had the greatest spectral resolution. A seven-by-three factorial experiment was used to investigate the effect of seven temperatures (5,10,20,30,40,50, and 60°C) on three separate samples atpH 6.5,9.0, or 10.0. Spectra resolution was greatest at pH 10.0 and 20°C. 229

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Section: Lyopliilization Resolubilization and Titrationmentioning

confidence: 99%

Optimization of sample ph and temperature for phosphorus‐31 nuclear magnetic resonance spectroscopy of poultry manure extracts

Crouse

Sierzputowska-Gracz

Mikkelsen

2000

Communications in Soil Science and Plant Analysis

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“…As expected for pure DMPC dispersions a CSA decrease (around 18 -20 ppm) was measured on pure DMPC systems by increasing the temperature (and the membrane fluidity) with the transition-related jump around 297 K [48]. This was also the case for MET containing MLV (MET/DMPC ratios of 1/10 and up to 1/4 M/M, middle and top traces, column A).…”

Section: P-nmr Experiments In MLVmentioning

confidence: 53%

Anticushing Drug Metyrapone Exhibits Specific Interactions with Serine Containing Systems. A Possible Molecular Target?

Crouzier¹,

Debouzy²,

Nachon³

et al. 2011

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Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) is a drug largely used as inhibitor of glucocorticoid synthesis. Although its binding to various proteins has been well indentified, its accurate molecular mechanism of action remains unknown. Therefore, the interactions of metyrapone (MET) with various membrane components such as phospholipids, cholesterol, their corresponding polar heads and a model serine containing peptide have been investigated by NMR and ESR methods. It was found that neither cholesterol nor most of the phospholipids tested, nor dimyristin exhibit any interaction with MET, except phosphatidylserine (DMPS). Furthermore, only serine bearing polar head (O-phosphoserine)showed an association with MET (stoechiometry 1:1, Kd = 3200M-1). As similar observations were also performed on serine alone and in the presence of the serine containing model peptide, (NASDSDGQDL), a possible implication of these interactions in the binding recognition of MET on serine-containing active site was finally tested and discussed.

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Neutral Trichlorooxorhenium(V) Complexes Containing New Heterofunctionalized Phosphane Ligands of the Type PN2 and PNO

Correia¹,

Domingos²,

Santos³

2000

Eur. J. Inorg. Chem.

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Appendixes: Selective Compilation of 31P-NMR Data

Cited by 7 publications

References 81 publications

Optimization of sample ph and temperature for phosphorus‐31 nuclear magnetic resonance spectroscopy of poultry manure extracts

Optimization of sample ph and temperature for phosphorus‐31 nuclear magnetic resonance spectroscopy of poultry manure extracts

Anticushing Drug Metyrapone Exhibits Specific Interactions with Serine Containing Systems. A Possible Molecular Target?

Neutral Trichlorooxorhenium(V) Complexes Containing New Heterofunctionalized Phosphane Ligands of the Type PN2 and PNO

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