Apple phlorizin oxidation product 2 inhibits proliferation and differentiation of 3T3-L1 preadipocytes

Wang, Xue; Wang, Juan; Wei, Lina; Hu, Ching Yuan; Deng, Hong; Guo, Yurong; Meng, Yong Hong

doi:10.1016/j.jff.2019.103525

Cited by 8 publications

(10 citation statements)

References 46 publications

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“…According to our previous work (Wang et al, 2019a), phloretin (400 μg/mL) shows good capacity on keeping the quality of salmon fillets through decreasing the microbial community diversity, biogenic amines formation, and lipid oxidation. Our another work (Wang et al, 2019b) proved that phlorizin and its oxidation product 2 (POP2) inhibits proliferation and differentiation of 3T3-L1 preadipocytes by decreasing adipocyte viability and restraining the accumulation of intracellular triglycerides.…”

Section: Antibacterial Mechanism Of Apple Phloretin On Physiological mentioning

confidence: 92%

“…Among numerous flavonoids, phloretin belongs to the dihydrochalcones class, a type of polyphenol found in plants, particularly in the bark, leaves, and fruit of apple trees (Chang et al, 2012). It is known to exert a wide variety of biological activities such as anti-inflammatory, antioxidant, and anticancer activities (Chang et al, 2012;Ng et al, 2019;Wang et al, 2019b). The antimicrobial activity of phloretin has been studied and variable results have been reported on different microbial species.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial mechanism of apple phloretin on physiological and morphological properties of Listeria monocytogenes

et al. 2022

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Green bactericides are excellent strategies for reducing food spoilage. Phloretin was regarded as efficient, broad-spectrum and safe preservative, which showed enormous potential in food industry. Inhibitory mechanism of phloretin on Listeria monocytogenes was systematically elucidated through integrity of cell membrane, intracellular protein content, energy level, and nucleic acid morphological character. SEM and TEM results showed minimal injury of cell membrane, which was further proved by a leakage of potassium ions, ATP and little protein and nucleic acid. There were drastic reduction of intracellular or total protein and ATP compared to cell without phloretin treatment. However, the specific activity of hexokinase and isocitrate dehydrogenase were unaffected after phloretin treatment. From these results, we concluded that phloretin's bacteriostatic mechanism is decreasing the intracellular protein content and energy level resulting from DNA aggregation. This finding was prospective to fully illustrate the phloretin's mechanism and develop it into a natural preservative in food industry.

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Section: Antibacterial Mechanism Of Apple Phloretin On Physiological mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Antibacterial mechanism of apple phloretin on physiological and morphological properties of Listeria monocytogenes

et al. 2022

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“…According to the Folin–Ciocalteu method, the total polyphenol content in YAP was determined and expressed as gallic acid equivalent (mg GAE/g) ( Chen L. Y. et al, 2015 ). The individual phenol compounds were analyzed by HPLC according to our previous method ( Wang et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Thinned-Young Apple Polyphenols Inhibit Halitosis-Related Bacteria Through Damage to the Cell Membrane

et al. 2022

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The thinned young apple is a by-product and is generally discarded in the orchard during fruit thinning. The polyphenol content of thinned young apples is about 10 times more than that of ripe apples. In our study, the antibacterial effect of thinned young apple polyphenols (YAP) on the halitosis-related bacteria including Porphyromonas gingivalis, Prevotella intermedius, and Fusobacterium nucleatum was investigated. The minimum inhibitory concentrations of YAP against P. gingivalis, P. intermedia, and F. nucleatum were 8.0, 8.0, and 12.0 mg/ml, while the minimum bactericidal concentrations were 10.0, 10.0, and 14.0 mg/ml, respectively. The scanning electron microscopy and transmission electron microscopy analyses showed that after YAP treatment, the membrane surface of halitosis-related bacterial cells was coarse and the cell wall and membrane were separated and eventually ruptured. The integrity of the cell membrane was determined by flow cytometry, indicating that the cells with the integrity membrane significantly reduced as the YAP concentration treatment increased. The release of proteins and nucleic acids into the cell suspension significantly increased, and the membrane potential reduced after the YAP treatment. This research illustrated the antibacterial mechanism of YAP against halitosis-related bacteria and provided a scientific basis of utilizing the polyphenols from the discarded thinned young apples.

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“…Many studies in recent years have shown the prospects of bioactive compounds from various plant sources as an alternative treatment for obesity by regulating various aspects of adipocyte differentiation and apoptosis, without any noticeable adverse effects on human physiology (Borah et al, 2021; Jung et al, 2011; Lahrita et al, 2015; Liu et al, 2021). For example, dietary polyphenols/bioactive compounds such as phlorizin, catechin, sulforaphane, withanolides, and hispidulin or plants extracts (Jiang et al, 2019; Lee et al, 2018; Ranaweera et al, 2021; Sung et al, 2019; Wang et al, 2019; Wu et al, 2019) have been well proven to interfere with the adipogenesis as well to reduce the lipid accumulation in murine 3T3‐L1 cells. Therefore, consuming a diet enriched in anti‐adipogenic substances can be a potential strategy in the prevention and treatment of obesity.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of transcriptional factors such as peroxisome proliferator-activated receptors (PPARs), CCTTA/enhancer-binding proteins (C/EBPs), and sterol regulatory element-binding protein 1c (ADD1/SREBP-1c) in adipose tissues plays a vital role in adipocyte differentiation(Chae et al, 2015).While C/EBPβ is an early marker of adipocyte differentiation and is necessary for the binding of other adipogenic transcriptional factors, both PPARγ and C/EBPα are considered as the master regulators of the adipogenesis process and are expressed in the later stages of cell differentiation These key differentiation regulators are responsible for the induction of adipocyte target genes such as leptin, adiponectin, and FAS, which in turn regulate various aspects of triglyceride synthesis and their accumulation in differentiated adipocytes. PPARγ is also involved in the regulation of insulin resistance and glucose metabolism, and its expression is promoted by the expression of C/EBPα(Wang et al, 2019). Several past studies have shown that the expression of C/EBPα, C/EBPβ, and PPARγ are all necessary for the adipogenesis process, and their reduction or knockdown can inhibit this process and further accumulation of lipids(Cristancho & Lazar, 2011;Janson et al, 2021;Morrison & Farmer, 2000).…”

mentioning

confidence: 99%

Comparative evaluation of Brassica oleracea , Ocimum basilicum , and Moringa oleifera leaf extracts on lipase inhibition and adipogenesis in 3T3‐L1 adipocytes

Nallamuthu

Jain

Anand

2022

Journal of Food Biochemistry

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The anti-adipogenic effects of alcohol extract of Brassica oleracea (BO), Ocimum basilicum (OB), and Moringa oleifera (MO) leaves on 3T3-L1 pre-adipocyte as well as their lipase inhibitory properties were comparatively evaluated. The polyphenol-rich extracts of MO, BO, and OB leaves were profiled for major bioactive compounds using UPLC-HRMS/MS analysis. Among the three plant extracts, BO had the highest flavonoid content with stronger radical scavenging activity. All extracts exhibited lipase inhibitory activities, dose-dependently and a non-competitive type of inhibition with altered kinetic parameters was observed. In cell culture studies, these plant extracts remarkably inhibited the intracellular triglyceride accumulation in 3T3-L1 cells and the effect was in the decreasing order of BO > OB > MO. The extracts also downregulated the key transcription factors of adipogenesis (PPARγ, C/EPBα, PI3k, p-Akt, and FAS enzyme) as well as modulated the release profile of leptin and adiponectins.Overall, BO extract showed an exceptional inhibitory potential against both adipogenesis and lipase activities among the evaluated plants. Practical applicationsThe present study highlights the anti-adipogenic potential of three local edible plants and herbs, that is, Brassica oleracea (BO), Ocimum basilicum (OB), and Moringa oleifera (MO) leaves, using in vitro models. The results revealed that the BO extract had remarkable activity against adipogenesis in the 3T3-L1 pre-adipocyte differentiation model as well as lipase inhibitory properties. This study elucidates the prospects of natural plant-derived compounds in managing obesity-related health issues. The outcomes of this study would be useful in developing alternative or complementary therapies that are being preferred and largely sought over pharmacological treatments and procedures for controlling and treating abnormal weight gain in humans.

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Apple phlorizin oxidation product 2 inhibits proliferation and differentiation of 3T3-L1 preadipocytes

Cited by 8 publications

References 46 publications

Antibacterial mechanism of apple phloretin on physiological and morphological properties of Listeria monocytogenes

Antibacterial mechanism of apple phloretin on physiological and morphological properties of Listeria monocytogenes

Thinned-Young Apple Polyphenols Inhibit Halitosis-Related Bacteria Through Damage to the Cell Membrane

Comparative evaluation of Brassica oleracea , Ocimum basilicum , and Moringa oleifera leaf extracts on lipase inhibition and adipogenesis in 3T3‐L1 adipocytes

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