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The carotenoids contents of pumpkin were determined in this study by spectrophotometric method. This method was applied on beta-carotene as standard solution as well as samples. The absorption were determined at λmax 454 nm, the analytical method was validated by evaluating linearity, precision, accuracy, LODs, and LOQs of the analyts. Beer’s law is obeyed over the concentration of (0.3-9) µg/ml with a linear regression correlation coefficient of 0.9986, and recovery in range from 98.5-102.2%. After the validation was performed using standard solution, the method then was applied on samples of pumpkin available in local market in Syria after extraction the carotenoids by using Acetone/ Dichloromethane 70/30 .The results ranged between 216.46 - 80.56 µg/g. During boiling for pumpkin, the level of carotenoid reduced between 0.45-13.26 %, 0.18 – 23.07%, 17.3-40.54%, 22.65-51.73%, 14.91_55.82% and 26.16 – 72.56%, for 5, 10, 15, 30, 45 and 60 min respectively. According to the obtained result results, it is concluded that the boiling reduced carotenoid at different rates.
The carotenoids contents of pumpkin were determined in this study by spectrophotometric method. This method was applied on beta-carotene as standard solution as well as samples. The absorption were determined at λmax 454 nm, the analytical method was validated by evaluating linearity, precision, accuracy, LODs, and LOQs of the analyts. Beer’s law is obeyed over the concentration of (0.3-9) µg/ml with a linear regression correlation coefficient of 0.9986, and recovery in range from 98.5-102.2%. After the validation was performed using standard solution, the method then was applied on samples of pumpkin available in local market in Syria after extraction the carotenoids by using Acetone/ Dichloromethane 70/30 .The results ranged between 216.46 - 80.56 µg/g. During boiling for pumpkin, the level of carotenoid reduced between 0.45-13.26 %, 0.18 – 23.07%, 17.3-40.54%, 22.65-51.73%, 14.91_55.82% and 26.16 – 72.56%, for 5, 10, 15, 30, 45 and 60 min respectively. According to the obtained result results, it is concluded that the boiling reduced carotenoid at different rates.
The purpose of this research is to study the present data on the effect of different dosages of vitamin A (retinol acetate) on the metabolism and localization of protein substances (protein) in the body of experimental animals (fattening bull calves). The authors use the concept of conversion of protein substances (protein) from the diet feed into the main nutrients of the edible part of the carcass (cuts) of bull calves fed on diets with pulp granules. The authors evaluate the morphological parameters of the animal after slaughter. For the experiment, 45 heads of black-and-white bull calves at the age of 12-13 months with a live weight of 270-290kg were selected. They were distributed according to the principle of similarity into three experimental groups of 15 heads each. The groups differed in diet, namely, an increase in the dosage of vitamin A in the second and third groups by 25 and 30% relative to the first group. According to the slaughter data and the study of the morphological composition of carcasses, the authors found that it was necessary to introduce vitamin A preparation into such diets. In this experiment, the authors used retinol acetate with the biological activity of 1 mln international units in 1 g, so that their content in the diets was 23-24 thousand international units per 100kg of live weight (optimally derived dose, depending on the live weight of the animal during the experiment). This contributed to an increase in the average daily weight gain of bull calves by 11.7% and an increase in carcass weight by 9.2%, including flesh weight by 11.1%. In the carcass flesh, the protein content increased by 15.4%, fat by 15.2%, and energy by 8.2%. The conversion rate of protein substances (protein) of the feed into the edible protein of the flesh of the carcass increased by 1.33% and the energy of the feed by 1.2%.
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