Application of 2-octyl-cyanoacrylate for corneal perforation and glaucoma filtering bleb leak

Abstract

BackgroundThis paper reports on the efficacy of a tissue adhesive, 2-octyl-cyanoacrylate, in the treatment of corneal perforation and glaucoma filtering bleb leak.MethodsTwo eyes from two patients with corneal perforation or laceration and two eyes from two patients with bleb leak were included. The patients underwent application of 2-octyl-cyanoacrylate onto the perforated or leaking site, and a hydrogel contact lens was applied as a bandage. We also evaluated the in vitro cell toxicity of 2-octyl-cyanoacrylate in HeLa cells.ResultsThe two cases of corneal perforation were repaired within 22 days with one application of the tissue adhesive. The two cases of bleb leak were repaired with 2–4 applications of the tissue adhesive over 134 (range 17–134) days). There were no recurrences or adverse effects during a mean follow-up period of 12.7 months. In vitro testing revealed that 2-octyl-cyanoacrylate was markedly toxic to HeLa cells.ConclusionFour patients with corneal perforation or bleb leak were successfully managed using 2-octyl-cyanoacrylate tissue adhesive. This simple and easy surgical technique may become an alternative therapeutic option for corneal perforation or bleb leak, although several applications of this tissue adhesive may be required. Although 2-octyl-cyanoacrylate was toxic to HeLa cells, no adverse clinical effects were noted using this adhesive.

Hyperdry human amniotic membrane is useful material for tissue engineering: Physical, morphological properties, and safety as the new biological material

“…The hyperdry device and sterilization by γ‐irradiation at 25 kGy, which has been recommended for terminal sterilization of medical products, can completely prevent known transmissible infections, and no adverse effects have been reported in patients who received HD‐AM grafts . Bacteriologic test also indicated that 25 kGy γ‐irradiation achieved to complete sterilization(data not shown).…”

“…No recurrence of pterygium was noted, and no signs of infection and rejection were observed during the follow-up period. HD-AM has been found to be a safe and useful biological material for human ocular surface disease, [29][30][31]37 for human dura mater undergoing neurosurgery, 32 for human bony surface the mastoid cavity in canal wall down tympanoplasty, 33,34 and for surgical defects of the human oral mucosa. 35,36 Many surgeons said that HD-AM is easy to care for treatment and it is able to short the operating time.…”

“…The hyperdry device and sterilization by g-irradiation at 25 kGy, which has been recommended for terminal sterilization of medical products, 50 can completely prevent known transmissible infections, and no adverse effects have been reported in patients who received HD-AM grafts. [29][30][31][32][33][34][35][36][37] Bacteriologic test also indicated that 25 kGy g-irradiation achieved to complete sterilization(data not shown). To manage unexpected risks, however, it is essential to accurately record in the recipient's case notes all unique donation numbers of all grafts used, to store samples corresponding to each donation number, and to undertake long-term monitoring of the recipients after the operation.…”

“…HD‐AM was also shown to reduce wound dehydration. The details of these benefits are described in clinical observations and case reports …”

“…The details of these benefits are described in clinical observations and case reports. [29][30][31][32][33][34][35][36][37] The purposes of the present study were to (1) compare the physical and histological properties of HD-AM with those of freeze-dried human amniotic membrane (FD-AM) processed with typical freeze-dry methods, (2) evaluate the safety of HD-AM, and (3) demonstrate the feasibility of HD-AM transplantation in a case of recurrent pterygium.…”