Application of a 32-Microband Electrode Array Detection System for Liquid Chromatography Analysis

Abstract

An electrode array consisting of 32 microband electrodes and a generator electrode was constructed. With this electrode array, both the oxidation and reduction three-dimensional hydrodynamic chromatovoltammograms of a reversible reaction can be obtained in a single voltammetric run. The versatility of this electrode array was demonstrated by running solutions containing various phenolic compounds with an FIA and an HPLC system. From the oxidation and reduction chromatovoltammograms obtained, individual components in the sample solution can be determined quantitatively, even when overlapping of the chromatography peaks occurred. With this microband electrode array, detection limits down to 2.0 × 10(-8) M for the studied phenolic compounds were obtained.

Electrochemical Detection Based on Redox Cycling Using Interdigitated Microarray Electrodes at µL/min Flow Rates

“…Chao and Huang [4], for example, used an array detector consisting of 32 microbands to obtain 3D hydrodynamic chrono-and chromatovoltammograms in¯ow injection analysis (FIA) and liquid chromatography (LC). Multichannel electrochemical detection in FIA has also been demonstrated by Matsue et al [5], while a multichannel potentiostat for potential control of up to 16 microelectrodes for stripping analysis purposes was described by Paeschke et al [6].…”

Immunoassays based on microelectrodes arrayed on a silicon chip for high throughput screening of liver fibrosis markers in human serum

“…MEMS processed microelectrode array, e.g., interdigitated array (IDA), has been employed in electrochemical biosensors, electronic tongues or electronic noses, and detectors of capillary electrophoresis or liquid chromatography for its high sensitivity derived from the precisely controlled pattern (Chao and Huang, 1997;Gavin and Ewing, 1996;Laureyn et al, 2000;Sangodkar et al, 1996;Yang et al, 2004). Particularly, arrayed immunosensors coupled with electrochemical detectors have attracted extensive interests for its powerful potential in simultaneously probing disease relevant markers (Kojima et al, 2003).…”

Use of Disposable GRC Electrodes for the Detection of Phenol and Chlorophenols in Liquid Chromatography

“…5,6 Electrochemical detection coupled with HPLC can be an ideal approach for such tasks because phenol and CPs are electroactive and can be detected with high sensitivity. 9,10 Moreover, the high selectivity of electrochemical detection often requires less sample-pretreatment, in turn allowing rapid and inexpensive detection. On the other hand, however, because high electrode potentials were required to observe the useful oxidation signals, the electrodes became rapidly fouled due to the adsorption of dimeric or polymeric oxidation products.…”