Immunoassays based on microelectrodes arrayed on a silicon chip for high throughput screening of liver fibrosis markers in human serum

Shi, Mianhong; Peng, Yunhui; Zhou, Jia; Liu, Baohong; Huang, Yixiao; Kong, Jilie

doi:10.1016/j.bios.2005.11.011

Cited by 36 publications

(7 citation statements)

References 33 publications

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“…Traditional analytical methods for detecting protein biomarkers such as TGF-β are based on immunoassays, including western blotting and enzyme-linked immunosorbent assay; however, these assays have limitations in real-time monitoring due to the long sample pretreatment process and the complexity of sample labeling. To overcome these drawbacks, various electrochemical and optical immunosensor platforms, including amperometric, , voltametric, , fluorescent, , and plasmonic − sensors, have been broadly studied to achieve rapid monitoring. Usually, optical sensing probes such as fluorescent tags are advantageous for intracellular monitoring of protein biomarkers; however, their low photostability and photobleaching are problematic in long-term cellular monitoring.…”

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confidence: 99%

Extra- and Intracellular Monitoring of TGF-β Using Single Immunoplasmonic Nanoprobes

et al. 2021

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Transforming growth factor-β (TGF-β) is a well-known disease-related biomarker associated with fibrotic diseases, and initiation and progression of cancer in many organs. Therefore, quantitative and sensitive detection of TGF-β and similar biomarkers is crucial for patient treatment in the early stages of diagnosis. In many studies, the detection of TGF-β, an important profibrotic and cancer promoting cytokine, has been generally conducted by fluorescence or absorbance-based immunoassays. However, conventional methods for detecting TGF-β have problems including use of time-consuming sample pretreatment steps and multiple reagents for signal amplification and difficulty in real-time detection from living cells. Herein, we present a plasmon-based immunoassay for TGF-β using antibody-conjugated single gold nanoparticles that act as optically excellent intracellular and extracellular detection probes that do not require additional signal amplification. To detect TGF-β sensitively and selectively, we exploited the localized surface plasmon resonance (LSPR) property of antibody-conjugated plasmonic gold nanoparticles at a single particle level. By measuring the LSPR spectral shifts of the single plasmonic nanoprobes, TGF-β can be detected down to the picomolar level, which is comparable with the conventional methods but without significant interference from other proteins. The optimized plasmonic nanoprobes were applied to quantify and monitor the extracellular TGF-β level secreted from the cells under stress conditions, such as cancer, and exposure to toxic environments. Owing to the ease of cellular internalization of the nanoprobes, we directly image and detect increases in intracellular TGF-β levels in living cells under the given stress conditions without cell lysis. We envision that this strategy of using individual nanoparticles as sensors to monitor protein biomarkers in living cells could be applied for various biological assays and diagnosis.

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confidence: 99%

Extra- and Intracellular Monitoring of TGF-β Using Single Immunoplasmonic Nanoprobes

et al. 2021

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“…Recently, miniaturized, label-free electrochemical immunoassay protein chips (Shi et al, 2006;Yang et al, 2004) have been showing remarkable advantages of low cost and no requirement for additional immunochemicals, which provides enhanced sensitivity and specificity. And the recognition of An-Ab interaction can be detected via measuring the change of capacitance, potential, current and/or resistance (Jiang et al, 2003;Liu et al, 2004;Sargent and Sadik, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…1 shows photos and sketch of the silicon chip integrated with arrayed disk Au working electrodes (WEs), Au counter electrode (CE) and Ag/AgCl reference electrode (RE). The fabrication procedure was slightly different from our previous work (Shi et al, 2006), i.e. Ag electrode was prepared with the same procedure as WEs and CE, while replacing Au with Ag and depositing 10 nm nickel between Ti and Ag to ensure best adhesive effect.…”

Section: Introductionmentioning

confidence: 99%

“…And the recognition of An-Ab interaction can be detected via measuring the change of capacitance, potential, current and/or resistance (Jiang et al, 2003;Liu et al, 2004;Sargent and Sadik, 1999). In our previous work, we developed a multichannel electrochemical immunoassay system basing on interdigitated arrays (IDAs) of microelectrodes on a silicon chip to detect a drop of serum sample (Shi et al, 2006). Enjoying the advantage of co-deposition of desired antibody and polypyrrole at the exact position via electrochemical operation, simultaneously detection of multitarget antigens was successively achieved on a silicon chip.…”

Section: Introductionmentioning

confidence: 99%

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Multianalyte immunoassay based on insulating-controllable PoPD film at arrayed electrodes integrated on a silicon chip

Shi

Peng

Zhou

et al. 2007

Biosensors and Bioelectronics

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“…Furthermore, by integrating various sensors and actuators into the chips, organs-on-a-chip provide a tunable biochemical and biomechanical cellular environments. 10–22…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Cardiac Microsystems for Pathophysiological Studies and Drug Screens

et al. 2015

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Microfabricated organs-on-chips consist of tissue-engineered 3D in vitro models, which rely on engineering design and provide the physiological context of human organs. Recently, significant effort has been devoted to the creation of a biomimetic cardiac system by using microfabrication techniques. By applying allometric scaling laws, microengineered cardiac systems simulating arterial flow, pulse properties, and architectural environments have been implemented, allowing high-throughput pathophysiological experiments and drug screens. In this review, we illustrate the recent trends in cardiac microsystems with emphasis on cardiac pumping and valving functions. We report problems and solutions brought to light by existing organs-on-chip models and discuss future directions of the field. We also describe the needs and desired design features that will enable the control of mechanical, electrical, and chemical environments to generate functional in vitro cardiac disease models.

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Immunoassays based on microelectrodes arrayed on a silicon chip for high throughput screening of liver fibrosis markers in human serum

Cited by 36 publications

References 33 publications

Extra- and Intracellular Monitoring of TGF-β Using Single Immunoplasmonic Nanoprobes

Extra- and Intracellular Monitoring of TGF-β Using Single Immunoplasmonic Nanoprobes

Multianalyte immunoassay based on insulating-controllable PoPD film at arrayed electrodes integrated on a silicon chip

Biomimetic Cardiac Microsystems for Pathophysiological Studies and Drug Screens

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