Application of a gold electrode, modified by a self-assembled monolayer of 2-mercaptodecylhydroquinone, to the electroanalysis of hemoglobin

Abstract

A gold electrode modified by a self-assembled monolayer of 2-mercaptodecylhydroquinone (H(2)Q(CH(2))(10)SH) was applied to investigate the electrochemical response of hemoglobin in aerated buffer solutions. Compared with a bare gold electrode, the monolayer of H(2)Q(CH(2))(10)SH could suppress the reduction wave of dissolved oxygen in the buffer while effectively promoting the rate of electron transfer between hemoglobin and the electrode. Thus, a convenient way for electroanalysis of hemoglobin in air was achieved at the H(2)Q(CH(2))(10)SH/Au electrode. A linear relationship existed between peak current and concentration of hemoglobin in the range 1 x 10(-7)-1 x 10(-6) mol L(-1).

Liquid phase deposition of hemoglobin/SDS/TiO2 hybrid film preserving photoelectrochemical activity

“…To facilitate the electron transfer between protein and electrode, numerous efforts have been devoted to provide a desirable protein-electrode interface environment in which the electron transfer can be probed [1]. Although the direct electron transfer of Hb has been achieved on various film-coated electrodes or chemically modified-electrodes such as sol-gel films [2,3], ion liquids [4], clay [5], nanomaterials [6][7][8][9], and self-assembled monolayers [10][11][12], there are two routine methods namely cast and adsorption used to immobilize Hb on the electrode surface.…”