Application of a modified BCR sequential extraction (three-step) procedure for the determination of extractable trace metal contents in a sewage sludge amended soil reference material (CRM 483), complemented by a three-year stability study of acetic acid and EDTA extractable metal content

Abstract

This paper provides additional data on a sewage sludge amended soil certified reference material, CRM 483, which was certified in 1997 for its EDTA and acetic acid extractable contents of some trace metals, following standardised extraction procedures. The additional work aimed to test the long-term stability of the material and the applicability of an improved version of the BCR three-step sequential extraction procedure on the sewage sludge amended soil (CRM 483). The paper demonstrates the CRM 483 long-term stability for EDTA and acetic acid extractable contents of Cd, Cr, Cu, Ni, Pb and Zn and gives the results (obtained in the framework of an interlaboratory study) for the extractable contents of the same elements in the CRM 483, following the BCR three-step sequential extraction scheme. The aqua regia extractable contents following the ISO 11466 Standard are also given. The data are given as indicative (not certified) values.

Chromium immobilization by extraradical mycelium of arbuscular mycorrhiza contributes to plant chromium tolerance

“…Briefly, soil Cr(VI) concentrations were determined according to EPA method 3060A (USEPA, 1996), DTPA-extractable Cr concentrations were determined by using ICP-OES after extracting Cr from soil by 0.005 M DTPA-0.01 M CaCl 2 -0.1 M triethanolamine (TEA) solution (pH 7.30) (Lindsay and Norvell, 1978), acid-extractable and reducible Cr were extracted separately by 0.11 mol/L acetic acid and 0.5 mol/L hydroxylammonium chloride according to European community bureau of reference (BCR) three-step sequential extraction procedure (Rauret et al, 2000), and then analyzed by ICP-OES. Nylon net (or 0.45 mm filter membrane) was used to separate the cultivation system into 3 compartments: root compartment (RC) for plant growth, buffer compartment (BC), and hyphal compartment (HC) for hyphal development only.…”

Comparative study of optimised BCR sequential extraction scheme and acid leaching of elements in the certified reference material NIST 2711

“…The application of partial (single) and sequential extractions as a means of assessing sediment quality has also resulted in the development and certification of new reference materials, such as BCR 483 and BCR 484 (sewage sludge amended soils certified for EDTA and acetic acid extractions) [18], and BCR 701 (lake sediment certified for BCR 3-step sequential extraction) [19,20]. A number of other reference materials, including NIST 2711 (soil certified for total metals and non-certified EPA Method 3050 leachable concentrations), have been used for assessing sequential extraction procedures.…”

Heavy Metal Bioavailability and Bioaccessibility in Soil

“…However, other reagents are also used and include the use of ammonium nitrate, calcium chloride and sodium nitrate. The extraction protocols, using EDTA, CH 3 COOH and CaCl 2 , used are based on those developed by the Standard, Measurements and Testing Program (formerly BCR) of the European Community (3-5) and subsequently re-evaluated for EDTA and acetic acid (6). 2.…”

“…The procedure adopted for the sequential extraction of metals from soil/sediments is based on three distinct stages (6). In stage 1 (exchangeable fraction), the metals released are representative of those that are the most bioavailable (and hence most mobile).…”