Application of a new hybrid organic–inorganic monolithic column for efficient deoxyribonucleic acid purification

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

“…The sample stacking was widely used to concentrate the analytes at the front of a capillary column with inline mode, which can be formed by constructing a small monolith bed at the front of a CE capillary or by coupling a short monolith capillary column to the inlet of a CE capillary [9]. Zhou et al [50] presented the use of a new hybrid monolithic column prepared in situ by polymerization of N-(␤-aminoethyl)-␥-aminopropyl-triethoxysilane and TEOS via sol-gel process for extraction of DNA following CE separation. The main extraction mechanism was based on the Coulombic force between DNA and the amino groups of monolithic column.…”

“…As shown in Fig. 3, trialkoxysilanes with hydrophobic organic moieties, such as N-octadecyldimethyl [3-(trimethoxy silyl)propyl]ammonium chloride [23], n-octyltriethoxysilane (C8-TES) [32][33][34][35], phenyltriethoxysilane (PTES) [36][37][38], propyltrimethoxysilane (C3-TMS) [39], MTMS [40][41][42][43][44], and other trialkoxysilanes with active organic functionalities, such as aminopropyl triethoxysilane (APTES) [45][46][47][48][49], N-(␤-aminoethyl)-␥-aminopropyl-triethoxysilane [50], N-(␤-aminoethyl)-␥-aminopropylmethyldimethoxysilane [51], 3-chloropropyltrimethoxysilane [52], 3-mercaptopropyltrime thoxysilane (MPTMS) [53], N-trimethoxysilylpropyl-N,N,Ntrimethylammonium chloride [54], allyl-trimethoxysilane [ 55,56], and vinyltrimethoxysilane (VTMS) [57] have been selected as coprecursors with tetraalkoxysilanes, TMOS, or TEOS. Additionally, bridged silanes such as 1,4-bis(triethoxysily1)benzene [58] have also been used for preparation of hybrid monoliths.…”

Rapid recovery of DNA from agarose gel slices by coupling electroelution with monolithic SPE

“…Recently, we developed an amino silica monolithic column by in situ polymerization of tetraethoxysilane (TEOS) and N-(b-aminoethyl)-g-aminopropyltriethoxysilane (AEAPTES) for efficient DNA extraction [28]. The extraction procedure was carried out in a totally aqueous system, free from the use of organic solvents and high concentration salts, and thus could provide high-quality DNA for genetic analysis.…”

“…The amount of the recovered DNA was quantified by CE with LIF detection [28]. A 42-cm (35 cm to the detector) uncoated fused-silica capillary (Yongnian Optical Fiber Factory, Hebei, China) with 75 mm id and 375 mm od was used for DNA separation.…”

“…1A). The monolithic column was prepared by in situ polymerization of TEOS and AEAPTES in ethanol aqueous solution with CTMAB as the porogenic reagent [28]. Briefly, 225 mL ethanol, 75 mL water and 0.0222 g CTMAB were mixed in a 1.5 mL eppendorf tube and ultrasonicated for 5 min at 01C.…”