Application of a Nitric Oxide Sensor in Biomedicine

Saldanha, Carlota; Almeida, José Pedro; Silva-Herdade, Ana Santos

doi:10.3390/bios4010001

Cited by 17 publications

(12 citation statements)

References 114 publications

(148 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown in vitro that erythrocytes, in the presence of ACh, the natural substrate of the membrane AChE, are able to release NO, which can be quantified by an amperometric method with an amiNO-sensor [ 8 , 60 ]. It was also verified that the AChE–ACh enzyme active complex activates the protein kinase C (PKC), which phosphorylates the protein tyrosine kinase (PTK) transforming it from an inactive to an active state by covalent modulation ( Figure 1 ) [ 61 , 62 ].…”

Section: Enrolment Of Erythrocyte Ache In the Signal Transduction mentioning

confidence: 99%

Human Erythrocyte Acetylcholinesterase in Health and Disease

Saldanha

2017

Molecules

Self Cite

View full text Add to dashboard Cite

The biochemical properties of erythrocyte or human red blood cell (RBC) membrane acetylcholinesterase (AChE) and its applications on laboratory class and on research are reviewed. Evidence of the biochemical and the pathophysiological properties like the association between the RBC AChE enzyme activity and the clinical and biophysical parameters implicated in several diseases are overviewed, and the achievement of RBC AChE as a biomarker and as a prognostic factor are presented. Beyond its function as an enzyme, a special focus is highlighted in this review for a new function of the RBC AChE, namely a component of the signal transduction pathway of nitric oxide.

show abstract

Section: Enrolment Of Erythrocyte Ache In the Signal Transduction mentioning

confidence: 99%

Human Erythrocyte Acetylcholinesterase in Health and Disease

Saldanha

2017

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…The NO biosensor amiNO-sensor can be utilized in suspensions of endothelial cells from umbilical cord to quantify the NO liberated [13,14].…”

Section: Physiological Erythrocytes Properties-in Vitro Studies Mimicmentioning

confidence: 99%

Physiological properties of human erythrocytes in inflammation

Saldanha

Silva-Herdade

2017

JCB

View full text Add to dashboard Cite

Abstract. The aim of this mini review was to show data that will could open new biotechnical applications. Physiological properties of the erythrocytes obtained in inflammatory conditions simulated in vitro or in vivo in experimental animal models of inflammation or ex vivo by analysis performed in blood samples from patients with vascular diseases are herein presented. Those data obtained in vivo have been utilized in mathematical models of vascular blood circulation on search biomechanical properties of blood components.Behind the enlargement of scientific knowledge all data could open new questions giving support to create new therapeutic drugs and to ameliorate apparatus for non-invasive detection of vascular circulatory diseases and further surgical interventions.Kewords: Erythrocyte deformability, inflammation, nitric oxide Physiological erythrocytes properties-in vitro studies mimicking inflammatory acute conditionsInflammation or inflammatory response is a natural process of the organism to fight an aggressive internal or external chemical, physical or mechanical stimuli or infection resulting from virus or bacterium presences. Redness, edema, pain and heat are the four cardinal signals of inflammation well known since the antiquity. In case of tissue injury, release of chemical signals like histamine, prostaglandins and nitric oxide (NO) by endothelial cells occurs. NO liberate to vessel lumen is scavenged by erythrocytes through band 3 protein [1]. The NO diffuse also to adjacent smooth muscle cells inducing relaxation [2]. Vasodilation occurs, increases blood flow, augment vessel wall permeability and leukocytes recruitment with transmigration to cell injury site for phagocytes pathogens and cells debris. Vascular endothelial cells changes its phenotypes to participate in the acute inflammation which involve a fast and a slower responses in close relation with the blood cells [3]. Acute phase proteins like fibrinogen, oxLDL as well as the non-cholinergic molecule acetylcholine (ACh) levels increase in plasma [4][5][6]. It was showed that the erythrocyte when in presence of ACh, the natural of substrate of the membrane acetylcholinesterase (AChE) release NO which efflux is quantified by amperometric measurement with amiNO-sensor [7,8]. It was verified that the AChE-ACh enzyme active complex activate the protein kinase C (PKC) which in turn phosphorylate the protein tyrosine kinase (PTK) turning it from inactive to active state. PTK phosphorylate band 3 protein became able to receive NO in its thiol group from S-nitrosohemoglobin for delivery to extracellular medium [9][10][11][12]. PKC inhibited by phosphorylation protein tyrosine phosphatase (PTP) and also the phosphodiesterase 3 (PDE3) which consequently do not hydrolyzes the cyclic adenosine triphosphate (cAMP) [9,10]. The AChE-ACh

show abstract

“…The redox current is proportional to the NO concentration outside the membrane and is continuously monitored with the electrochemical detection system and connected to a computer [41]. For more details see the review about the application of NO sensors im medicine [42]. A higher NO release from erythrocyte samples obtained from patients with diseases related with hypoxia and inflammatory states, namely sickle cell disease, hypercholesterolemic and hypertensive patients was verified, besides the fact that they sustain impaired erythrocyte deformability [43].…”

Section: Erythrocyte Bioavailability In Oxygen and Nitric Oxidementioning

confidence: 99%

Instrumental analysis applied to erythrocyte properties

Saldanha¹

2015

JCB

Self Cite

View full text Add to dashboard Cite

The properties, structure and functions of the erythrocyte or red blood cell (RBC) have been known and evaluated by using simpler to more sophisticated technical devices. Microscopy, flow cytometry, spectrometry, spectroflurometry, aggregometer, ektacytometer zeta potential, amperometry, electrophoresis and centrifugation, are apparatus used to qualitatively and quantitatively characterize RBC, based on the principles of light transmission, light scattering spectroscopy, light absorption, fluorescence, light polarization, shear stress, shear rate, charge and molecular weight gradients. There is a symbiosis between the chemical, the physical and the mechanical principles of those instrumental analysis and the RBC properties to be measured. Here we briefly exemplify the relationship between the biochemical, biophysical and mechanical based techniques and the properties of the erythrocyte in healthy and disease.

show abstract

Application of a Nitric Oxide Sensor in Biomedicine

Cited by 17 publications

References 114 publications

Human Erythrocyte Acetylcholinesterase in Health and Disease

Human Erythrocyte Acetylcholinesterase in Health and Disease

Physiological properties of human erythrocytes in inflammation

Instrumental analysis applied to erythrocyte properties

Contact Info

Product

Resources

About