Instrumental analysis applied to erythrocyte properties

Abstract

The properties, structure and functions of the erythrocyte or red blood cell (RBC) have been known and evaluated by using simpler to more sophisticated technical devices. Microscopy, flow cytometry, spectrometry, spectroflurometry, aggregometer, ektacytometer zeta potential, amperometry, electrophoresis and centrifugation, are apparatus used to qualitatively and quantitatively characterize RBC, based on the principles of light transmission, light scattering spectroscopy, light absorption, fluorescence, light polarization, shear stress, shear rate, charge and molecular weight gradients. There is a symbiosis between the chemical, the physical and the mechanical principles of those instrumental analysis and the RBC properties to be measured. Here we briefly exemplify the relationship between the biochemical, biophysical and mechanical based techniques and the properties of the erythrocyte in healthy and disease.

Glycoprotein IIB-IIIA inhibitor, monafram decelerate the early phase of red blood cells aggregation

“…The reversible aggregation of red blood cells is a well-known determinant of hemorheological properties and microcirculation, especially under pathological conditions [2,3,11]. So, investigation of mechanisms of RBC aggregation continues to be of the primary scientific and clinical interest, and yet it is far from being resolved [16]. RBCs are considered to interact with each other due to intercellular bridges of polymer molecules [8] or osmotic forces [14], and, according to both explanations, RBC aggregation mechanisms are nonspecific.…”

An Updated Review of Methods and Advancements in Microvascular Blood Flow Imaging

“…Confocal microscopy has been used to study the principles of vessel regression , erythrocyte properties , detecting skin lesions in oncology , and in studying the effect of glutamic acid on the blood–brain barrier and the neurovascular unit in glutaric acidemia I . It has also been used to quantify capillary cell blood flow and in evaluating cutaneous microcirculation and dermal changes in systemic sclerosis .…”

Relationship between red blood cell membrane disorders and Doppler ultrasound indices of renal blood flow in patients with chronic kidney disease

“…Вступ. З прогресуванням хронічної хвороби нирок (ХХН) підвищується проникливість судинної стінки капілярів та дрібних судин нирок, відбувається сповільнення швидкості кровотоку, погіршуються реологічні властивості крові, що погіршує проникливість судинної стінки, посилює агрегацію та пригнічує властивості еритроцитів крові [1][2][3]. А за наявності цукрового діабету (ЦД) усі вищеперераховані процеси зростають максимально за рахунок порушення обмінних процесів в організмі хворих [4][5][6].…”