For site-specific soil ecological risk assessments (SERAs), an integrated chemical, ecotoxicological, and ecological analysis needs to be performed. The SERA guidelines of international institutions and countries recommend that a SERA be initiated at the screening level to save time and social economic cost; however, they provide no unified test species for this screening level. This study performed SERAs for field soils and confirmed the importance of selecting bioassay test species that reflect the ecotoxicity of field soils at the screening level. To confirm test species that reflect the ecological risk of field soils, correlation analysis was performed on the results of each bioassay with the integrated ecotoxicological risk index (EtoxRI). Our results showed that soil algae, nematodes, and plants were the most representative species in soil assays, with high correlation coefficients with EtoxRI. The results imply the importance of selecting test species that represent ecological risk for the screening level of SERAs. Based on these findings, when using SERAs, species sensitivity, ecological relevance, and economic aspects should be considered when selecting the bioassay test species.