Application of an online post-column derivatization HPLC-DPPH assay to detect compounds responsible for antioxidant activity in Sonchus oleraceus L. leaf extracts

Abstract

The online HPLC-DPPH radical assay was validated as a useful screening tool for investigating individual antioxidants in leaf extracts. Optimized extraction conditions were middle leaves pre-treated with liquid N(2), extraction at 25°C for 0.5 h and solvent removal by rotary evaporation.

Chicoric acid: chemistry, distribution, and production

“…The effects of tissue age may also vary with plant taxon. For example, in sowthistle ( Sonchus olearaceus L.), mature leaves contained higher levels of chicoric acid compared to close-to-the-base and young leaves (Ou et al, 2012). …”

Chicoric acid supplementation ameliorates cognitive impairment induced by oxidative stress via promotion of antioxidant defense system

“…[21][22][23][24][25][26] Chicoric acid has strong free radical scavenging and antioxidant activities. [27][28][29] Our previous study reported that chicoric acid and, to a lesser extent, its metabolites, caffeic acid and caaric acid, inhibited DPPHc, cOH, and ABTSc + free radicals. 30 A recent study found that chicoric acid could signicantly inhibit obesity induced by a high fat diet in C57BL/6J mice by enhancing the body's antioxidant defense system.…”

Elsholtzia ciliata (Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities

“…Model oxidation systems of DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid))radicals are highly effective, rapid, and possess other important advantages compared with conventional strategies for identification of antioxidants from complex mixtures [27]. There are a lot of studies that have determined antioxidant activity of essential oils, phenolic compounds, and others, but only a few researches have been published about the antioxidant activity of individual compounds [27,28]. E. ciliata is not an exception-there is only one publication from Liu et al describing the antioxidant effect of aqueous extracts from different parts of E. ciliata [29].…”