Application of Antibiotics/Antimicrobial Agents on Dental Caries

Qiu, Wei; Zhou, Yujie; Li, Zixin; Huang, Tu; Xiao, Yuhan; Cheng, Lei; Peng, Xian; Zhang, Lixin; Ren, Biao

doi:10.1155/2020/5658212

Cited by 98 publications

(86 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chlorhexidine showed the lowest binding affinity than any other ligand, which is the most effective widely-used antiplaque chemical that exhibited great activity against Gram-positive and Gram-negative bacteria and is known as the gold standard. 7 Based on binding affinity value, the lower the value, the better the result that will emerge. 34 β-sitosterol showed potential as an antibacterial agent by having a lower binding affinity value than other ligands, although it had a higher value than chlorhexidine, the gold standard.…”

Section: Discussionmentioning

confidence: 99%

“… 6 Many kinds of antibiotics and antimicrobial agents are used to prevent dental caries, among which the most widely employed are penicillin, tetracyclines, metronidazole, chlorhexidine, and fluoride. 7 Chlorhexidine is the most widely used antiseptic for mouth rinsing and is the gold-standard in dental practice. 8 Due to awareness of antibiotics resistance, plant extracts could be an alternative for an antibacterial agent.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bio-Mechanism Inhibitory Prediction of β-Sitosterol from Kemangi (Ocimum basilicum L.) as an Inhibitor of MurA Enzyme of Oral Bacteria: In vitro and in silico Study

Evangelina¹,

Herdiyati²,

Laviana³

et al. 2021

AABC

View full text Add to dashboard Cite

Background Dental caries is a widespread disease that causes dental tissue destruction and leads to local and general complications. Gram-positive bacteria including Streptococcus mutans , Streptococcus sanguinis , and Enterococcus faecalis take part in dental caries formation. Gram-positive bacteria have cell walls that consistof a thick layer of peptidoglycan which maintains the strength and rigidity of the bacteria, as well as bacteria guard from internal osmotic pressure. The biosynthesis of peptidoglycan involves many enzymes, including the Mur family, penicillin binding protein (PBP), and sortases. Purpose This research has the intention to screen and examine the antibacterial compound of edible plant Kemangi ( Ocimum basilicum L.) in terms of how it fights against some oral pathogenic bacteria of E. faecalis ATCC 29212, S. mutans ATCC 25175, and S. sanguinis ATCC 10566. Materials and Methods The O. basilicum L. was macerated by several organic solvents to obtain the extracts, before then being purified using several combinations of chromatography methods and the compound was discovered via spectroscopic methods. For the assay against bacteria, the extracts and compounds were tested using agar well diffusion and microdilution assay. Results The isolated compound was identified as β-sitosterol. The compound activity against bacteria was evaluated by in vitro assay against S. sanguinis ATCC 10566 and E. faecalis ATCC 29212 with the MIC and MBC value of 25,000 and 50,000 ppm, respectively. The compound was also tested by in silico study using the molecular docking method. The molecular interaction between β-sitosterol and the protein target showed a lower binding affinity value than the native ligand and other positive controls for each protein. Based on the amino acid residue bound to the ligands, β-sitosterol on MurA and SrtA is not competitive to the positive control, showing potential as a natural antibacterial agent. Meanwhile, on the MurB and PBP, β-sitosterol and positive control do compete with each other. Conclusion The compound, isolated from O. basilicum L. leaf, was determined as β-sitosterol, which has the molecular formula C 29 H 50 O. The antibacterial activity of β-sitosterol by in vitro assay showed weak antibacterial activity, yet exhibited the potential to inhibit the biosynthesis of peptidoglycan and prevent bacteria cell wall formation by inhibiting MurA and SrtA activity via docking simulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bio-Mechanism Inhibitory Prediction of β-Sitosterol from Kemangi (Ocimum basilicum L.) as an Inhibitor of MurA Enzyme of Oral Bacteria: In vitro and in silico Study

Evangelina¹,

Herdiyati²,

Laviana³

et al. 2021

AABC

View full text Add to dashboard Cite

show abstract

“…Erythromycin has bacteriostatic activities and is commonly prescribed for dental caries and dental plaque [ 55 , 56 , 58 ]. The most common microorganism that causes dental caries is Streptococcus mutans , which is highly sensitive to erythromycin [ 59 ].…”

Section: The Most Common Prescribed Antibioticsmentioning

confidence: 99%

Antibiotic Therapy in Dentistry

Ahmadi

Ebrahimi

Ahmadi

2021

International Journal of Dentistry

View full text Add to dashboard Cite

Dental caries, pulpal necrosis, trauma, and periodontal diseases can result in dental infections which could have severe consequences that affect both soft and hard tissues of the oral cavity. Dental infections commonly present with symptoms of pain, fever, and swelling. Surgical and endodontic treatments are the early management of infected teeth, followed by antibiotic therapy. Some alternative methods also exist for treating infection such as low-level laser therapy and photodynamic therapy. Antibiotics are generally used in dental procedures to treat odontogenic infections, nonodontogenic infections, local infection, focal infection, and prophylaxis. Antibiotic prophylaxis is prescribed for patients with immunosuppressed conditions, infective endocarditis, metabolic disorders, and patients with prosthetic joints. To reduce the complications of unnecessary antibiotic prescriptions especially bacterial resistance, comprehensive guidelines should be established. It has been noted that only about 12% of dentists adequately and correctly prescribe antibiotics, which shows the importance of comprehensive guidelines. Antibiotics prescription may result in some adverse effects such as hypersensitivity reactions and dermatological and allergic disorders. Furthermore, unnecessary prescription of antibiotics could result in several serious sequelae, for example, bacterial resistance, gastric and hematological problems, and diversion of bacterial microbiota. The present review attempts to summarize the indications of antibiotic therapy in dentistry and discuss the common types of antibiotics that are routinely used in dental practice based on pharmacologic classes. Moreover, types of antibiotics that are considered safe during pregnancy and childhood are also reviewed.

show abstract

“…Contributing a unique niche for the growth of microorganisms, their establishment in the oral cavity especially dental surfaces serves as etiological agents of dental caries [9][10][11]. There were a large number of reports that suggest irrational enhancement in prescribing antibiotics by medical professionals including dental surgeons that in turn contributes significantly amount to the growing burden of microbial resistance [12][13][14]. Confronted by increasing amounts of antibiotics use, appearance of opportunistic organisms intrinsically resistant to drugs that thwarts currently available treatment regimes makes diseases difficult and, sometimes, impossible to treat in hospitals and the community settings.…”

Section: Introductionmentioning

confidence: 99%

Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance

et al. 2020

View full text Add to dashboard Cite

The unprecedented use of antibiotics that led to development of resistance affect human health worldwide. Prescription of antibiotics imprudently and irrationally in different diseases progressed with the acquisition and as such development of antibiotic resistant microbes that led to the resurgence of pathogenic strains harboring enhanced armors against existing therapeutics. Compromised the treatment regime of a broad range of antibiotics, rise in resistance has threatened human health and increased the treatment cost of diseases. Diverse on metabolic, genetic and physiological fronts, rapid progression of resistant microbes and the lack of a strategic management plan have led researchers to consider plant-derived substances (PDS) as alternative or in complementing antibiotics against the diseases. Considering the quantitative characteristics of plant constituents that attribute health beneficial effects, analytical procedures for their isolation, characterization and phytochemical testing for elucidating ethnopharmacological effects has being worked out for employment in the treatment of different diseases. With an immense potential to combat bacterial infections, PDSs such as polyphenols, alkaloids and tannins, present a great potential for use, either as antimicrobials or as antibiotic resistance modifiers. The present study focuses on the mechanisms by which PDSs help overcome the surge in resistance, approaches for screening different phytochemicals, methods employed in the identification of bioactive components and their testing and strategies that could be adopted for counteracting the lethal consequences of multidrug resistance.

show abstract

Application of Antibiotics/Antimicrobial Agents on Dental Caries

Cited by 98 publications

References 151 publications

Bio-Mechanism Inhibitory Prediction of β-Sitosterol from Kemangi (Ocimum basilicum L.) as an Inhibitor of MurA Enzyme of Oral Bacteria: In vitro and in silico Study

Bio-Mechanism Inhibitory Prediction of β-Sitosterol from Kemangi (Ocimum basilicum L.) as an Inhibitor of MurA Enzyme of Oral Bacteria: In vitro and in silico Study

Antibiotic Therapy in Dentistry

Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance

Contact Info

Product

Resources

About