Application of antigenic biomarkers for Mycobacterium tuberculosis

Rodríguez-Hernández, Elba; Quintas-Granados, Laura Itzel; Flores-Villalva, Susana; Cantó-Alarcón, Jorge Germinal; Milián-Suazo, Feliciano

doi:10.1631/jzus.b2000325

Cited by 10 publications

(2 citation statements)

References 112 publications

(114 reference statements)

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“…To examine alterations in gene transcription during M.tb infection, we constructed an in vitro bacterial infection model [24]. A speci c antibody was employed to perform immuno uorescence staining of Ag85B, a conserved M.tb-speci c protein [25]. The ndings revealed a distinct Ag85B positive signal in THP-1 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Vitamin D inhibits apoptosis in THP-1 cells infected with mycobacterium tuberculosis through TNF signaling pathway

Yang,

Deng,

Liu

et al. 2024

Preprint

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Vitamin D (VD) has been extensively associated with the resistance against tuberculosis (TB); however, the mechanism underlying the reduction in TB susceptibility by VD remains uncertain. In our prior investigation, we discovered the relationship between VD and mycobacterium tuberculosis (M.tb)-induced aberrant osteoclastogenesis. Here we report that VD diminishes apoptosis in M.tb-infected THP-1 cells through tumor necrosis factor (TNF) signaling pathway. This novel perspective contributes to the elucidation of the intricate relationship between VD and tuberculosis. In this study, THP-1 cells were infected with the H37Rv strain for 4h at a MOI of 10 and then treated with 1,25-dihydroxy vitamin D (1,25(OH)2D3) (10− 6, 10− 8, 10− 10M) for 1d and 4d respectively. RNA sequencing (RNA-seq) was performed, and differential expression analysis was conducted by the R package edgeR. Immunofluorescence (IF) and immunohistochemistry (IHC) techniques were employed for VDR, TNFR1 and TUNEL in TB patients and serum levels of TNF-α and IL6 were measured simultaneously. Furthermore, the utilization of western blot and qRT-PCR techniques was employed to investigate the impact of VD on pivotal molecules involved in the TNF signaling pathway. In addition, Bacillus Calmette-Guérin (BCG) and VD were administrated by tail vein and articular cavity injection in vivo. Our findings revealed a robust responsiveness of the TNF signaling pathway to M.tb-induced inflammation, resulting in elevated expression of TNF-α, IL-6, and severe apoptosis. VD exhibited significant inhibitory effect on M.tb-induced inflammation and apoptosis both in vitro and in vivo. This study provides new ideas for vitamin D in the study of tuberculous bone destruction.

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Section: Resultsmentioning

confidence: 99%

Vitamin D inhibits apoptosis in THP-1 cells infected with mycobacterium tuberculosis through TNF signaling pathway

Yang,

Deng,

Liu

et al. 2024

Preprint

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“…Although sputum smear can identify activated TB quickly, but many patients cannot provide sputum samples for smear tests. The detectable rate was lower than 30% (false positive, lower sensitivity), hard to be used in children with TB or extra pulmonary tuberculosis diagnosis, it is hard to be used in children TB means getting a sputum sample from a child is not very convenient; the sputum culture test was the golden standard for TB diagnosis, but cost 3–4 weeks to get the results, was not meet the requirement of early diagnosis ( Rodríguez-Hernández et al, 2020 ; Yang et al, 2020 ). Molecular biology testing is fast and time-saving, but requires expensive equipment and testing personnel with high technical knowledge, but has the weakness to distinguish dead or active Mtb ( Rozales et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Development of a new cellular immunological detection method for tuberculosis based on HupB protein induced IL-6 release test

Jiang

et al. 2023

Front. Microbiol.

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ObjectiveAs a virulence factor, HupB plays important roles in the survival of MTB after infection and modulates the host immune response. In the current study, we aim to explore a new cellular immunological detection method for tuberculosis infection detection based on HupB protein.MethodsHupB was used to stimulate PBMCs extracted from pulmonary tuberculosis (PTB) patients, and secreted cytokines was examined. Then, we constructed a single center and a multi-center clinical trials to collect PBMCs from PTB patients, nPTB patients, or healthy volunteers to verify our findings.ResultsCytokine’s screening illustrated that IL-6 was the only cytokine released after HupB stimulation. Single-center and multi-center clinical trials showed that HupB stimulation significantly increased the level of IL-6 in the supernatant of PBMCs from PTB patients. Then we compared the specificity and sensitivity of HupB induced IL-6 release assay with ESAT-6 and CFP10 induced interferon γ release assay (IGRA), and found in smear positive PTB patients, the specificity and sensitivity of HupB induced IL-6 release assay was better than IGRA, and in smear negative PTB patients, the sensitivity was better. Combination of both assays provided an improved specificity and sensitivity for tuberculosis diagnosis.ConclusionThis study explored an immunological detection method for tuberculosis infection cells based on HupB protein-induced IL-6 release test, which can be used to enhance the diagnosis diagnostic accuracy of TB.

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Integrating untargeted and targeted LC–MS‐based metabolomics to identify the serum metabolite biomarkers for tuberculosis

Sa,

Ding,

Zhang

et al. 2024

Biomedical Chromatography

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Given the limitations of untargeted metabolomics in precise metabolite quantification, our current research employed a novel approach by integrating untargeted and targeted metabolomics utilizing ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry (UHPLC‐QTOF‐MS/MS) to analyze the metabolic profile and potential biomarkers for tuberculosis (TB). A cohort of 36 TB patients and 36 healthy controls (HC) was enlisted to obtain serum samples. Multivariate pattern recognition and univariate statistical analysis were employed to screen and elucidate the differential metabolites, whereas dot plots and receiver operating characteristic (ROC) curves were established for the identification of potential biomarkers of TB. The results indicated a distinct differentiation between the two groups, identifying 99 metabolites associated with five primary metabolic pathways in relation to TB. Of these, 19 metabolites exhibited high levels of sensitivity and specificity, as evidenced by the area under curve values approaching 1. Following targeted quantitative analysis, three potential metabolites, namely, L‐asparagine, L‐glutamic acid, and arachidonic acid, were demonstrated excellent discriminatory ability as evidenced by the results of the ROC curve, dot plots, and random forest model. Particularly noteworthy was the enhanced diagnostic efficacy of the combination of these three metabolites compared to singular biomarkers, suggesting their potential utility as serum biomarkers for TB diagnosis.

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Application of antigenic biomarkers for Mycobacterium tuberculosis

Cited by 10 publications

References 112 publications

Vitamin D inhibits apoptosis in THP-1 cells infected with mycobacterium tuberculosis through TNF signaling pathway

Vitamin D inhibits apoptosis in THP-1 cells infected with mycobacterium tuberculosis through TNF signaling pathway

Development of a new cellular immunological detection method for tuberculosis based on HupB protein induced IL-6 release test

Integrating untargeted and targeted LC–MS‐based metabolomics to identify the serum metabolite biomarkers for tuberculosis

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