Application of Antimicrobial Proteins and Peptides in Developing Disease‐Resistant Plants

Nandi, Ashis Kumar

doi:10.1002/9781118867716.ch3

Cited by 7 publications

(5 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a general rule, the deployment of genetic engineering approaches that involve the expression of two or more antimicrobial gene products, including PR proteins, in a specific crop should provide more effective and broad-spectrum disease control than the single-gene strategy 24–27 . The efficiency of PR genes in transgenic approaches to obtain pathogen resistance is well documented (for a review see 17,28,29 ). Numerous transgenic plant developments have been reported in which varying degrees of protection against specific fungal and/or oomycete pathogens have been achieved.…”

Section: Introductionmentioning

confidence: 99%

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Boccardo

Segretin

Hernández

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani , Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae . Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R . solani in greenhouse conditions and, furthermore, they are protected against P . hyoscyami f.sp. tabacina and P . nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Boccardo

Segretin

Hernández

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The function of PR genes should be further studied by transgenic method. The efficiency of PR genes in transgenic approaches to obtain pathogen resistance is well documented [ 60 , 61 ]. Furthermore, significant constitutive expression of PRs in transgenic plants overexpressing PR genes accompanied by increased resistance to pathogens.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Two Novel PR4 Genes from Picea asperata

Zhao

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

Pathogenesis-related (PR) proteins are important in plant pathogenic resistance and comprise 17 families, including the PR4 family, with antifungal and anti-pathogenic functions. PR4 proteins contain a C-terminal Barwin domain and are divided into Classes I and II based on the presence of an N-terminal chitin-binding domain (CBD). This study is the first to isolate two PR4 genes, PaPR4-a and PaPR4-b, from Picea asperata, encoding PaPR4-a and PaPR4-b, respectively. Sequence analyses suggested that they were Class II proteins, owing to the presence of an N-terminal signal peptide and a C-terminal Barwin domain, but no CBD. Tertiary structure analyses using the Barwin-like protein of papaya as a template revealed structural similarity, and therefore, functional similarity between the proteins. Predictive results revealed an N-terminal transmembrane domain, and subcellular localization studies confirmed its location on cell membrane and nuclei. Real-time quantitative PCR (RT-qPCR) demonstrated that PaPR4-a and PaPR4-b expression levels were upregulated following infection with Lophodermium piceae. Additionally, PaPR4-a and PaPR4-b were induced in Escherichia coli, where the recombinant proteins existed in inclusion bodies. The renatured purified proteins showed antifungal activity. Furthermore, transgenic tobacco overexpressing PaPR4-a and PaPR4-b exhibited improved resistance to fungal infection. The study can provide a basis for further molecular mechanistic insights into PR4-induced defense responses.

show abstract

“…have shown profound beneficial effects in agriculture to enhance plant growth and induction of disease resistance [ [24] , [48] , [49] ]. Various elicitors have been documented, and the most central amongst them are the cell wall elicitors [ 50 ]. The elicitors either directly or indirectly induce resistance in plants against pathogen infection through Microbe Associated Molecular Pattern (MAMPS) or Pathogenesis-related (PR) resistance [ [51] , [52] , [53] ].…”

Section: Discussionmentioning

confidence: 99%

Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator

Britto

Joshi

Jogaiah

2021

Biotechnology Reports

View full text Add to dashboard Cite

Highlights Mycogenic cell wall elicitor was isolated from trichoderma atroviride . The isolated elicitor was identified as trehalose by LC-MS analysis. Seed priming with elicitor enhanced early germination and vigour. Primed plants induced resistance against leaf spot disease of brocolli. Trehalose sugar act as a bio-stimulant for growth promotion and plant defence.

show abstract

Application of Antimicrobial Proteins and Peptides in Developing Disease‐Resistant Plants

Cited by 7 publications

References 160 publications

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials

Cloning and Characterization of Two Novel PR4 Genes from Picea asperata

Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator

Contact Info

Product

Resources

About