Application of APP/PS1 Transgenic Mouse Model for Alzheimer’s Disease

Abstract

Alzheimer's disease (AD), the most common neurodegenerative disorder, will not only reduce quality of life severely, but also bring heavy economic burden to the family and society. Slow progress in AD therapies partially due to lack of appropriate animal models. APP/PS1 transgenic mouse, a widely used animal model for AD, can be used in lots of aspects for AD related study, such as neuronal apoptosis, inflammation, cholinergic abnormal, neurogenesis disorder and synaptic plasticity. Despite all this, APP/PS1 transgenic mice model is not a perfect model, and more suitable animal model according to the aim of research should be established. mutant, the APP KI mutation alone does not affect markers for adult hippocampal neurogenesis [12]. PS1 KI mice, a model that shows cognitive decline developed in an Aβ-independent way, therefore plaque-dependent pathology cannot be expected [13].Tg models are important models for the AD study. They are established on the basis of genetics, mainly involves amyloid precursor protein(APP) gene on chromosome 21, presenilin1 (PS1) gene on chromosome 14, presenilin 2 (PS2) gene on chromosome 1, Tau protein gene on chromosome 17 and Apolipoprotein E (ApoE) gene on chromosome 19 [14]. By transgenesis, the course of AD can be simulated steadily at molecular level. Meanwhile, this technique can produce many animals at the same time, so the reliability and repeatability of experimental results can be ensured. Tg models have three types: single transgenic models such as APP Tg mouse model, double Tg models such as APP/PS1 Tg mouse model and triple Tg mouse models such as APP/ PS1/Tau Tg mouse model [15]. Although the emergence of Tg model is a hot spot of AD research in recent years, there are still problems in the application of Tg AD models, such as lack of aging process, poor reproductive ability and immunity. Therefore, compared with the real AD pathological changes, there is still a long way to go. APP/PS1 Tg mouse model

Assessment of pathological features in Alzheimer’s disease brain tissue with a large field-of-view visible-light optical coherence microscope

“…Mouse models, in particular, have been extensively used as their breeding and handling is rather easy and cheap, and the genetic modification techniques are well established. In the past decades, a variety of mouse models have been developed, which reproduce different aspects of AD-related pathologies 8 , 9 . The mouse model used in this paper exhibits an overexpression of the amyloid precursor protein (APP) and a presenilin 1 (PS1) mutation accompanied by a rapid development of A-β plaques in various cortical regions 8 , 10 …”

Multifunctional liposomes delay phenotype progression and prevent memory impairment in a presymptomatic stage mouse model of Alzheimer disease

“…The treatment ended at 12 months of age, when untreated mice display AD-like cognitive, anatomical and biochemical feature [20,26,27].…”

Métodos Experimentais Para Estudos Não Clínicos Da Doença De Alzheimer

“…O modelo duplo transgênico (APP/PS1) possui indução de duas proteínas, a percussora amiloide (APP) no cromossomo 21, que integra a membrana e intermedeia as sinapses entre neurônios, e a presenilina 1 (PS1) presente no cromossomo 14, que está relacionada com o desenvolvimento cerebral (LI et al, 2015).…”

“…A linha cruzada entre gene APP e PS possuem pelo menos 5 (cinco) tipos: APPswe × PS1, APPSL × PS1M146L, APPswe × PS-1dE9, 5 × FAD e APPSL × PS1ki. Contudo, o modelo mais utilizado é APPswe × PS1dE9 (LI et al, 2015). Esses tipos do modelo (APP/ PS1) em função do tempo de vida do animal mimetizam a inflamação, neurotoxicidade de Aβ e apoptose dos neurônios.…”