Application of autologous periosteal cells for the regeneration of class III furcation defects in Beagle dogs

Abstract

The aim of this study was to evaluate the healing of class III furcation defects following transplantation of autogenous periosteal cells combined with b-tricalcium phosphate (b-TCP). Periosteal cells obtained from Beagle dogs' periosteum explant cultures, were inoculated onto the surface of b-TCP. Class III furcation defects were created in the mandibular premolars. Three experimental groups were used to test the defects' healing: group A, b-TCP seeded with periosteal cells were transplanted into the defects; group B, b-TCP alone was used for defect filling; and group C, the defect was without filling materials. Twelve weeks post surgery, the tissue samples were collected for histology, immunohistology and X-ray examination. It was found that both the length of newly formed periodontal ligament and the area of newly formed alveolar bone in group A, were significantly increased compared with both group B and C. Furthermore, both the proportion of newly formed periodontal ligament and newly formed alveolar bone in group A were much higher than those of group B and C. The quantity of cementum and its percentage in the defects (group A) were also significantly higher than those of group C. These results indicate that autogenous periosteal cells combined with b-TCP application can improve periodontal tissue regeneration in class III furcation defects.

Periodontal Regeneration Using Periodontal Ligament Stem Cell-Transferred Amnion

“…[3][4][5] MSC from various tissues have been investigated for cell-based regeneration of periodontal tissues, including the periodontal ligament, 6-8 bone marrow, [9][10][11] adipose tissue, 12 and periosteum. [13][14][15] Cultured stem cells from the periodontal ligament (PDLSCs) possess characteristics of MSC such as cell surface MSC marker expression (CD105 + , CD90 + , CD44 + , CD73 + , CD45 -, CD31 -, and CD34 -) and multilineage differentiation ability (i.e., into osteoblasts, chondrocytes, and adipocytes). [16][17][18] In addition to MSC characteristics, PDLSCs have unique properties to form a cementum/ periodontal ligament complex-like structure when ectopically transplanted in animals.…”

Leptin Overexpression in Bone Marrow Stromal Cells Promotes Periodontal Regeneration in a Rat Model of Osteoporosis

“…β‐TCP possesses a porous structure and prominent bioresorbable properties. Bone‐like tissue was successfully formed in nude mice, 20 and Class III furcation defects were repaired in beagle dogs 41 using β‐TCP. Therefore, porous β‐TCP was used for the in vivo experiments in the current study.…”

Periodontal ligament stem cells: an update and perspectives

“…There have been reports used on ceramic and synthetic polymers in periodontal regeneration. However, biomaterials might cause rejections and foreign‐body reactions, which might have a negative impact on expected outcomes.…”

“…94,95 In their study, Tsunmanuma et al showed that canine periodontal ligament cell sheets combined with a mixture of beta tricalcium phosphate and collagen enhanced the cementum and periodontal ligament fiber formation in a canine 1-wall defect model. 77 There have been reports used on ceramic 96,97 and synthetic polymers 98,99 in periodontal regeneration. However, biomaterials might cause rejections and foreign-body reactions, which might have a negative impact on expected outcomes.…”