Periodontal ligament stem cells: an update and perspectives

Pandula, P.K. Chamila Prageeth; Samaranayake, LP; Jin, Lijian; Zhang, Chengfei

doi:10.1111/jicd.12089

Cited by 28 publications

(17 citation statements)

References 101 publications

(184 reference statements)

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“…Since the isolation and identification of multipotent postnatal stem cells from human PDL tissue 10 years ago (Seo et al 2004), these cells, defined as PDLSCs, have become an interesting adult stem cell source for regenerative dentistry because of their ease of isolation from discarded or removed teeth, particularly those teeth extracted for impaction or orthodontic reasons (Chamila Prageeth Pandula et al 2014;Bright et al 2015). Furthermore, these cells can differentiate into osteoblasts, chondrocytes, neurons and muscle cells, among others.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, these cells can differentiate into osteoblasts, chondrocytes, neurons and muscle cells, among others. PDLSCs are derived from ectomesenchymal origin, are formed during tooth development and have been investigated for the repair of tissues not only within but also outside the mouth (Chen et al 2012;Chamila Prageeth Pandula et al 2014). In particular, many preclinical and clinical studies have suggested that PDLSCs represent an effective therapeutic tool for periodontal regeneration (Bright et al 2015;Bassir et al 2016).…”

Section: Discussionmentioning

confidence: 99%

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Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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In this study, we extensively screened the in vitro and in vivo effects of PDLSCs following short-term inflammatory and/or hypoxic pretreatments. We found that the 24-h hypoxic pretreatment of PDLSCs significantly enhanced cell migration and improved cell surface CXCR4 expression. In addition, hypoxia-pretreated PDLSCs exhibited improved cell colony formation and proliferation. Cells that were dually stimulated also formed more colonies compared to untreated cells but their proliferation did not increase. Importantly, the hypoxic pretreatment of PDLSCs enhanced cell differentiation as determined by elevated RUNX-2 and ALP protein expression. In this context, the inflammatory stimulus impaired cell OCN protein expression, while dual stimuli led to decreased RUNX-2 and OCN mRNA levels. Although preconditioning PDLSCs with inflammatory and/or hypoxic pretreatments resulted in no differences in the production of matrix proteins, hypoxic pretreatment led to the generation of thicker cell sheets; the inflammatory stimulus weakened the ability of cells to form sheets. All the resultant cell sheets exhibited clear bone regeneration following ectopic transplantation as well as in periodontal defect models; the amount of new bone formed by hypoxia-preconditioned cells was significantly greater than that formed by inflammatory stimulus- or dual-stimuli-treated cells or by nonpreconditioned cells. The regeneration of new cementum and periodontal ligaments was only identified in the hypoxia-stimulus and no-stimulus cell groups. Our findings suggest that PDLSCs that undergo short-term hypoxic pretreatment show improved cellular behavior in vitro and enhanced regenerative potential in vivo. The preconditioning of PDLSCs via combined treatments or an inflammatory stimulus requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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“…Periodontal ligament stem cells (PDLSCs) represent a further plastic stem cell population within the human oral cavity [108]. Notably, their differentiation capacity is not only limited to mesenchymal derivatives as they are able to generate ectodermal progeny [109][110][111][112].…”

Section: Other Adult Stem and Progenitor Cellsmentioning

confidence: 99%

Controversial Role of Toll-like Receptor 4 in Adult Stem Cells

Zeuner

Bieback

Widera

2015

Stem Cell Rev and Rep

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Adult or somatic stem cells are tissue-resident cells with the ability to proliferate, exhibit self-maintenance as well as to generate new cells with the principal phenotypes of the tissue in response to injury or disease. Due to their easy accessibility and their potential use in regenerative medicine, adult stem cells raise the hope for future personalisable therapies. After infection or during injury, they are exposed to broad range of pathogen or damage-associated molecules leading to changes in their proliferation, migration and differentiation. The sensing of such damage and infection signals is mostly achieved by Toll-Like Receptors (TLRs) with Toll-like receptor 4 being responsible for recognition of bacterial lipopolysaccharides (LPS) and endogenous danger-associated molecular patterns (DAMPs). In this review, we examine the current state of knowledge on the TLR4-mediated signalling in different adult stem cell populations. Specifically, we elaborate on the role of TLR4 and its ligands on proliferation, differentiation and migration of mesenchymal stem cells, hematopoietic stem cells as well as neural stem cells. Finally, we discuss conceptual and technical pitfalls in investigation of TLR4 signalling in stem cells.

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“…Lastly, like other MSC, PDLSC also show paracrine induction of angiogenesis in vitro and in vivo [32] . Thus, in addition to their multilineage potential, the possibility of an immortal cell line has made PDLSC highly promising for tissue engineering applications [5] .…”

Section: Background and Applicationsmentioning

confidence: 99%

“…MSC among craniomaxillofacial sources are diverse, unique and variably well characterized. These include dental pulp stem cells (DPSC) [4] , periodontal ligament stem cells (PDLSC) [5] , suture-derived mesenchymal stromal cells (SMC) [6] and gingival mesenchymal stem cells (GMSC) [7] ( Figure 2). …”

Section: Introductionmentioning

confidence: 99%

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

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Tissue specific mesenchymal stem cells are of great scientific interest across diverse fields in stem cell biology and medicine. Sources of mesenchymal stem cells (MSC) in the craniomaxillofacial skeleton are numerous and are of significant interest to craniofacial and oral surgeons, among other medical and dental specialties. Mesenchymal stem cells are defined by their characteristic cell surface marker expression, multipotentiality and ability for self-renewal. Bone marrow derived MSC (BMSC) are most commonly studied, but are of low abundance in the craniomaxillofacial (CMF) skeleton. Alternative CMF sources of MSC are diverse and include dental pulpal stem cells (DPSC), periodontal ligament stem cells (PDLSC), suture-derived mesenchymal stromal cells (SMC), gingival mesenchymal stem cells (GMSC)and adipose-derived MSC (ASC). This brief review will introduce various MSC sources derived from the head and neck, along with a discussion of their identity, characteristics and results of preclinical studies in tissue engineering.

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Periodontal ligament stem cells: an update and perspectives

Cited by 28 publications

References 101 publications

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Controversial Role of Toll-like Receptor 4 in Adult Stem Cells

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

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