Application of Bacteria in Concrete

Mondal, Sandip; Das, Palash; Chakraborty, Arun

doi:10.1016/j.matpr.2017.06.276

Cited by 30 publications

(12 citation statements)

References 6 publications

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“…Using the pour plate method, 0.1mL from dilutions 10 −5 and 10 −6 was plated on nutrient agar medium in basal media containing (w v-1) 0.5% peptone, 0.1% NaCl, 0.2% K 2 HPO 4 , 0.01% CaCl 2 , 0.01% MgSO 4 , 0.1% yeast extract, 1.5% bacteriological agar, and 1% glucose. Plates were kept in an incubator for 24 h at 370 • C. Morphologically different colonies were separated, and pure cultures were preserved at 40 • C [36,37].…”

Section: Isolation Of a Bacterial Strainmentioning

confidence: 99%

Development of Bacterium for Crack Healing and Improving Properties of Concrete under Wet–Dry and Full-Wet Curing

et al. 2020

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Concrete cracking is inevitable, coupled with increased permeability, exacerbating the adverse impacts of atmospheric conditions and chemical attacks. Calcium carbonate precipitation resulting from certain microorganisms’ metabolism is a novel approach that can self-heal the cracks and improve concrete properties. In this study, the development and effect of bacteria Bacillus cohnii on crack healing, regained compressive strength after pre-cracking, sorptivity, water absorption, and concrete microstructures were investigated. For this purpose, a Bacillus cohnii bacterial concentration of 105 cells/mL was used as a water replacement in the concrete mixtures. Two methods subsequently cured the prepared concrete specimens: wet–dry (W-D) cycle and full-wet (F-W). In the wet–dry cycle, the cast specimens were immersed in water for 24 h and then kept at room temperature for 24 h, which was considered as one cycle; this process was repeated for 28 days. In the full-wet curing, specimens were immersed in water for 28 days. However, the curing water was changed every 24 h to facilitate the essential oxygen supply for bacterial activity to precipitate calcium carbonate. The results revealed that 90% and 88% surface healing was noticed in full-wet and full-dry pre-cracked specimens at 28 days.

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Section: Isolation Of a Bacterial Strainmentioning

confidence: 99%

Development of Bacterium for Crack Healing and Improving Properties of Concrete under Wet–Dry and Full-Wet Curing

et al. 2020

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“…A key requirement for successfully designing living materials is linking formulation conditions with final material properties and—concurrently—biological activity. [ 21 ] Material properties of general interest include those related to material formulation and processing (cure time, polymer fraction), mechanical properties (toughness, stiffness, fatigue strength), and transport properties (mass diffusivity, hydraulic permeability, etc.). Biological activity includes viability and motility of embedded organisms, ability to proliferate, responsivity of embedded organisms to signals of interest, or activity of secreted biologicals within or upon the embedding matrix.…”

Section: Introductionmentioning

confidence: 99%

Engineering Gelation Kinetics in Living Silk Hydrogels by Differential Dynamic Microscopy Microrheology and Machine Learning

et al. 2021

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Microbes embedded in hydrogels comprise one form of living material. Discovering formulations that balance potentially competing for mechanical and biological properties in living hydrogels—for example, gel time of the hydrogel formulation and viability of the embedded organisms—can be challenging. In this study, a pipeline is developed to automate the characterization of the gel time of hydrogel formulations. Using this pipeline, living materials comprised of enzymatically crosslinked silk and embedded E. coli—formulated from within a 4D parameter space—are engineered to gel within a pre‐selected timeframe. Gelation time is estimated using a novel adaptation of microrheology analysis using differential dynamic microscopy (DDM). In order to expedite the discovery of gelation regime boundaries, Bayesian machine learning models are deployed with optimal decision‐making under uncertainty. The rate of learning is observed to vary between artificial intelligence (AI)‐assisted planning and human planning, with the fastest rate occurring during AI‐assisted planning following a round of human planning. For a subset of formulations gelling within a targeted timeframe of 5–15 min, fluorophore production within the embedded cells is substantially similar across treatments, evidencing that gel time can be tuned independent of other material properties—at least over a finite range—while maintaining biological activity.

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“…In addition to modifying the viscosity of cementitious materials, a second important effect brought about by bacterial additives aims at improving the properties of the cured material. For a variety of different bacterial additives, e.g., bacterial cell walls, [ 184 ] and bacterial solutions, [ 185,186 ] such an improvement of the mechanical competence of the final, cured material has been reported. [ 187–189 ] Moreover, bacterial additives can enhance the corrosion resistance of load‐bearing steel elements in reinforced concrete [ 190–192 ] —and this increases the durability and thus lifetime of objects making use of this class of building materials, e.g., bridge pillars, walls in high rise buildings, and tunnel constructions.…”

Section: Applications Of Natural and Modified Biofilms In Different Fieldsmentioning

confidence: 99%

Bacterial Materials: Applications of Natural and Modified Biofilms

Hayta

Ertelt

Kretschmer

et al. 2021

Adv Materials Inter

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Over millennia, bacteria have developed clever strategies to build biopolymer‐based communities in which they can survive even extremely challenging conditions. Such bacterial biofilms come with a broad range of fascinating material properties that—in settings such as medicine, food production, or other areas of industry—make it difficult to remove or inactivate them: they can stick to many surfaces, repel water and oils, and can even transport electrons. Inspired by the outstanding versatility and sturdiness of such bacterial biofilms, material scientists have set out to harness those properties and to create bacterial materials for different applications. However, as the range of technological applications employing biofilms keeps expanding, improved material properties or broader functionalities are desired. Here, such attempts where materials with improved properties were created by making use of either natural or modified bacterial biofilms are reviewed. The areas in which those bacterial materials may be used range from agriculture and (environmental) biotechnology over biomedical and electrical engineering to construction engineering.

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Application of Bacteria in Concrete

Cited by 30 publications

References 6 publications

Development of Bacterium for Crack Healing and Improving Properties of Concrete under Wet–Dry and Full-Wet Curing

Development of Bacterium for Crack Healing and Improving Properties of Concrete under Wet–Dry and Full-Wet Curing

Engineering Gelation Kinetics in Living Silk Hydrogels by Differential Dynamic Microscopy Microrheology and Machine Learning

Bacterial Materials: Applications of Natural and Modified Biofilms

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