Bacterial Materials: Applications of Natural and Modified Biofilms

Hayta, Elif N.; Ertelt, Marvin Johannes; Kretschmer, Martin; Lieleg, Oliver

doi:10.1002/admi.202101024

Cited by 32 publications

(16 citation statements)

References 232 publications

(241 reference statements)

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“…Biomaterial encapsulation hinders the rapid removal of microorganisms and often their inactivation. Inspired by the versatility and strength of such biomaterials, scientists have developed hybrid materials for application in various areas, from agriculture and (environmental) biotechnology [ 7 ], biomedicine, and electrical engineering [ 8 ] to food production, synthetic chemistry, and bioelectronics [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

et al. 2022

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Microorganism-cell-based biohybrid materials have attracted considerable attention over the last several decades. They are applied in a broad spectrum of areas, such as nanotechnologies, environmental biotechnology, biomedicine, synthetic chemistry, and bioelectronics. Sol-gel technology allows us to obtain a wide range of high-purity materials from nanopowders to thin-film coatings with high efficiency and low cost, which makes it one of the preferred techniques for creating organic-inorganic matrices for biocomponent immobilization. This review focuses on the synthesis and application of hybrid sol-gel materials obtained by encapsulation of microorganism cells in an inorganic matrix based on silicon, aluminum, and transition metals. The type of immobilized cells, precursors used, types of nanomaterials obtained, and their practical applications were analyzed in detail. In addition, techniques for increasing the microorganism effective time of functioning and the possibility of using sol-gel hybrid materials in catalysis are discussed.

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Section: Introductionmentioning

confidence: 99%

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

et al. 2022

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“…Bacteria secrete various biomolecules to create extensive networks of extracellular matrix (ECM). These biofilms, often associated with pathogenic infections 1 , have gained popularities for their remarkable mechanical properties, transforming bacteria into elegant biofactories of smart materials 2 – 5 . The major components of the ECM of Escherichia coli (E. coli) biofilms are curli fibrils—bacterial functional amyloids 6 , 7 —and cellulose 8 , 9 (Fig.…”

Section: Introductionmentioning

confidence: 99%

Synthetic phosphoethanolamine-modified oligosaccharides reveal the importance of glycan length and substitution in biofilm-inspired assemblies

et al. 2022

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Bacterial biofilm matrices are nanocomposites of proteins and polysaccharides with remarkable mechanical properties. Efforts understanding and tuning the protein component have been extensive, whereas the polysaccharide part remained mostly overlooked. The discovery of phosphoethanolamine (pEtN) modified cellulose in E. coli biofilms revealed that polysaccharide functionalization alters the biofilm properties. To date, the pattern of pEtN cellulose and its mode of interactions with proteins remains elusive. Herein, we report a model system based on synthetic epitomes to explore the role of pEtN in biofilm-inspired assemblies. Nine pEtN-modified oligosaccharides were synthesized with full control over the length, degree and pattern of pEtN substitution. The oligomers were co-assembled with a representative peptide, triggering the formation of fibers in a length dependent manner. We discovered that the pEtN pattern modulates the adhesion of biofilm-inspired matrices, while the peptide component controls its stiffness. Unnatural oligosaccharides tune or disrupt the assembly morphology, revealing interesting targets for polysaccharide engineering to develop tunable bio-inspired materials.

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“…The biofilm state renders microbial cells highly resistant to harsh environmental factors, such as extreme temperature, extreme pH, high salinity, ultraviolet radiation, lack of nutrients, and antibiotics. Moreover, biofilms are sticky and viscoelastic due to the supramolecular structure of extracellular polymeric substances and therefore difficult to remove from surfaces [ 6 ]. Consequently, biofilms cause various problems in medical fields and food and other industries [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Degradation by Seashell-Derived Calcium Hydroxide and Hydrogen Peroxide

Hata

Bouda²,

Hiruma³

et al. 2022

Nanomaterials

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Microbial cells and self-produced extracellular polymeric substances assembled to form biofilms that are difficult to remove from surfaces, causing problems in various fields. Seashell-derived calcium hydroxide, a sustainable inorganic material, has shown high bactericidal activity even for biofilms due to its alkalinity. However, its biofilm removal efficacy is relatively low. Herein, we report a biofilm degradation strategy that includes two environmentally friendly reagents: seashell-derived calcium hydroxide and hydrogen peroxide. A biofilm model of Escherichia coli was prepared in vitro, treated with calcium hydroxide–hydrogen peroxide solutions, and semi-quantified by the crystal violet stain method. The treatment significantly improved biofilm removal efficacy compared with treatments by calcium hydroxide alone and hydrogen peroxide alone. The mechanism was elucidated from calcium hydroxide–hydrogen peroxide solutions, which suggested that perhydroxyl anion and hydroxyl radical generated from hydrogen peroxide, as well as the alkalinity of calcium hydroxide, enhanced biofilm degradation. This study showed that concurrent use of other reagents, such as hydrogen peroxide, is a promising strategy for improving the biofilm degradation activity of seashell-derived calcium hydroxide and will contribute to developing efficient biofilm removal methods.

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Bacterial Materials: Applications of Natural and Modified Biofilms

Cited by 32 publications

References 232 publications

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology

Synthetic phosphoethanolamine-modified oligosaccharides reveal the importance of glycan length and substitution in biofilm-inspired assemblies

Biofilm Degradation by Seashell-Derived Calcium Hydroxide and Hydrogen Peroxide

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