No abstract

In a previous development stage, mostly individual antibacterial activity was a target in the optimization of biologically active compounds and antiseptic agents. Although this targeting is still valuable, a new trend has appeared since the discovery of superhigh resistance of bacterial cells upon their aggregation into groups. Indeed, it is now well established that the great majority of pathogenic germs are found in the environment as surface-associated microbial communities called biofilms. The protective properties of biofilms and microbial resistance, even to high concentrations of biocides, cause many chronic infections in medical settings and lead to serious economic losses in various areas. A paradigm shift from individual bacterial targeting to also affecting more complex cellular frameworks is taking place and involves multiple strategies for combating biofilms with compounds that are effective at different stages of microbiome formation. Quaternary ammonium compounds (QACs) play a key role in many of these treatments and prophylactic techniques on the basis of both the use of individual antibacterial agents and combination technologies. In this review, we summarize the literature data on the effectiveness of using commercially available and newly synthesized QACs, as well as synergistic treatment techniques based on them. As an important focus, techniques for developing and applying antimicrobial coatings that prevent the formation of biofilms on various surfaces over time are discussed. The information analyzed in this review will be useful to researchers and engineers working in many fields, including the development of a new generation of applied materials; understanding biofilm surface growth; and conducting research in medical, pharmaceutical, and materials sciences. Although regular studies of antibacterial activity are still widely conducted, a promising new trend is also to evaluate antibiofilm activity in a comprehensive study in order to meet the current requirements for the development of highly needed practical applications.

Microorganism-cell-based biohybrid materials have attracted considerable attention over the last several decades. They are applied in a broad spectrum of areas, such as nanotechnologies, environmental biotechnology, biomedicine, synthetic chemistry, and bioelectronics. Sol-gel technology allows us to obtain a wide range of high-purity materials from nanopowders to thin-film coatings with high efficiency and low cost, which makes it one of the preferred techniques for creating organic-inorganic matrices for biocomponent immobilization. This review focuses on the synthesis and application of hybrid sol-gel materials obtained by encapsulation of microorganism cells in an inorganic matrix based on silicon, aluminum, and transition metals. The type of immobilized cells, precursors used, types of nanomaterials obtained, and their practical applications were analyzed in detail. In addition, techniques for increasing the microorganism effective time of functioning and the possibility of using sol-gel hybrid materials in catalysis are discussed.

One of the main indices of the quality of water is the biochemical oxygen demand (BOD). A little over 40 years have passed since the practical application of the first microbial sensor for the determination of BOD, presented by the Japanese professor Isao Karube. This time span has brought new knowledge to and practical developments in the use of a wide range of microbial cells based on BOD biosensors. At present, this field of biotechnology is becoming an independent discipline. The traditional BOD analysis (BOD5) has not changed over many years; it takes no less than 5 days to carry out. Microbial biosensors can be used as an alternative technique for assessing the BOD attract attention because they can reduce hundredfold the time required to measure it. The review examines the experience of the creation and practical application of BOD biosensors accumulated by the international community. Special attention is paid to the use of multiple cell immobilization methods, signal registration techniques, mediators and cell consortia contained in the bioreceptor. We consider the use of nanomaterials in the modification of analytical devices developed for BOD evaluation and discuss the prospects of developing new practically important biosensor models.

⎯The relevant sol-gel encapsulation methods to design ORMOSIL (organically modified silica) protective shells around Debaryomyces hansenii yeast cells have been studied. Encapsulation is based on spontaneous "cell-in-shell" 3D structures. The physiological activity of the sol-gel encapsulated cells was determined by measurement of the oxidative activity of a BOD biosensor containing ORMOSIL-encapsulated cells. It was shown that the shells around the microbial cells did not prevent substrate diffusion and had excellent protection from extreme environmental factors (heavy metal ions, high pH, and UV radiation). In addition, it was found that the BOD biosensor surpasses some other known biosensors in their basic characteristics, including sensitivity, stability, and reproducibility. These results are important for the development of innovative methods of cell encapsulation and the creation of biocatalysts used in biotechnology and ecology.