Application of baculoviruses as biopesticides and the possibilities of nanoparticle mediated delivery

Das, Sujit; Goswami, Arunava; Debnath, Nitai

doi:10.1016/b978-0-12-815829-6.00011-5

Cited by 7 publications

(5 citation statements)

References 64 publications

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“…Importantly, baculoviruses are more effective as delivery agents in controlling host genes than the passive delivery methods available for dsRNA-based insecticides [ 140 ]. The use of recombinant baculovirus strains to control pests has been proposed for decades and has recently gained more attention as sustainable biopesticides [ 141 , 142 , 143 ].…”

Section: Discussionmentioning

confidence: 99%

A Tale of Two Transcriptomic Responses in Agricultural Pests via Host Defenses and Viral Replication

Pantha

Subbaiah

et al. 2021

IJMS

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Autographa californica Multiple Nucleopolyhedrovirus (AcMNPV) is a baculovirus that causes systemic infections in many arthropod pests. The specific molecular processes underlying the biocidal activity of AcMNPV on its insect hosts are largely unknown. We describe the transcriptional responses in two major pests, Spodoptera frugiperda (fall armyworm) and Trichoplusia ni (cabbage looper), to determine the host–pathogen responses during systemic infection, concurrently with the viral response to the host. We assembled species-specific transcriptomes of the hemolymph to identify host transcriptional responses during systemic infection and assessed the viral transcript abundance in infected hemolymph from both species. We found transcriptional suppression of chitin metabolism and tracheal development in infected hosts. Synergistic transcriptional support was observed to suggest suppression of immune responses and induction of oxidative stress indicating disease progression in the host. The entire AcMNPV core genome was expressed in the infected host hemolymph with a proportional high abundance detected for viral transcripts associated with replication, structure, and movement. Interestingly, several of the host genes that were targeted by AcMNPV as revealed by our study are also targets of chemical insecticides currently used commercially to control arthropod pests. Our results reveal an extensive overlap between biological processes represented by transcriptional responses in both hosts, as well as convergence on highly abundant viral genes expressed in the two hosts, providing an overview of the host–pathogen transcriptomic landscape during systemic infection.

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Section: Discussionmentioning

confidence: 99%

A Tale of Two Transcriptomic Responses in Agricultural Pests via Host Defenses and Viral Replication

Pantha

Subbaiah

et al. 2021

IJMS

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“…Virion replicates or self-propagate in the cells of the insect's body so that eventually the insect dies because the whole body undergoes lysis. The infection is polyorganotrophic, which means the virus at the same time infects multiple tissues such as the epidermis, tracheal matrix, fat bodies, hemocytes, central nervous system cells, and pericardial (Das et al, 2019). Figure 3b shows the death larvae with their bodies were dried out.…”

Section: Treatmentmentioning

confidence: 99%

The Effect of Nanozeolite Concentration in a Delivery System of HaNPV1 to the Lethal Time against Crocidolomia pavonana

Prasetio¹,

Hermawan²,

Miranti³

et al. 2020

JTAS

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The constraints on the effectiveness of nuclear polyhedrosis virus (NPV) as biocontrol are usually due to environmental factors such as temperature and ultraviolet (UV) exposure. Zeolite has been commonly used as a carrier or delivery system for nuclear polyhedrosis viruses. In this study, zeolite powder was reduced into nanosized particles by beads milling method and was investigated for the effect of its concentration in the delivery system of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV1) on the lethal time against the larvae Crocidolomia pavonana. The formulation used three concentrations of nanozeolite suspension, 0.5, 1, 1.5, and 2 wt.% applied for each 4 × 107 of HaNPV1. A randomized block design (RBD) method was applied with 3 replications. The results showed that the scanning electron microscope (SEM) from nanozeolite was seen coating the entire surface of the HaNPV1 polyhedra and an increase of zeolite concentration caused acceleration of the lethal time of C. pavonana instar III. Thus, the fastest lethal time was 1.2 days receiving a concentration of 2 wt.%, which was significantly higher compared to without delivery (2.9 days). The increase of the zeolite concentration up to 2 wt.% in the delivery system for HaNPV1 improved their performance on lethal time and mortality against C. pavonana. It was concluded that nanozeolite as a delivery system enhanced and created a synergy in infecting C. pavonana.

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“…Further, baculoviruses are more effective as delivery agents in controlling host genes than the passive delivery methods available for dsRNAbased insecticides [192]. The use of recombinant baculovirus strains to control pests has been proposed for over decades and has recently gained more attention as sustainable biopesticides [193][194][195][196]. Transcript level inhibition of the juvenile hormone biosynthesis or alterations to its regulation is a common target attempted in recombinant baculoviruses developed as potential biopesticides [197,198].…”

Section: Key Host Genes Affected By the Acpnmv Infection Are Targetsmentioning

confidence: 99%

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Pantha

Subbaiah

et al. 2020

Preprint

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BackgroundAutographa californica Multiple Nucleopolyhedrovirus (AcMNPV) is a baculovirus with a high potential for its use as a biopesticide against arthropod pests. The budded form of the virus causes a systemic infection when it escapes the midgut to enter the hemolymph of susceptible hosts. Yet, the specific molecular processes underlying the biocidal activity of AcMNPV on its insect hosts are largely unknown.ResultsIn this study, we describe the transcriptional responses in two major pests, Spodoptera frugiperda and Trichoplusia ni, to determine the host-pathogen responses during AcMNPV infection, concurrently with the viral response to the host. We assembled species-specific de novo reference transcriptomes of the hemolymph to identify key transcripts that respond during pathogenesis in these arthropod models where genomic resources are sparse. We found that the suppression of transcriptional processes related to chitin, a metabolite critical for basement membrane stability and tracheal development are central in establishing a systemic infection. Synergistic transcriptional support was observed to suggest suppression of immune responses and induction of oxidative stress indicating disease progression in the host. The entire AcMNPV core genome was expressed in the host hemolymph and viral genes predominantly associated with the budded virus replication, structure, and movement were more abundant than those associated with the occlusion-derived virus. Genes known to directly arrest host cell cycle and development were among the most abundant AcMNPV transcripts in infected hosts. Interestingly, several of the host genes (e.g. Chitin synthase) that were targeted by the pathogen as revealed by our study are also targets of several chemical insecticides currently used commercially to control arthropod pests.ConclusionsOur results reveal an extensive overlap between biological processes represented by genes differently expressed in both hosts, as well as convergence on highly abundant viral genes expressed in the two hosts, providing an overview of the host-pathogen transcriptomic landscape during systemic infection. Given the diversity of AcMNPV strains that infect a wide range of insect hosts, our study provides a framework where pathogen strains could be selected to target specific host genes that facilitates modulation of the infection strength and specificity of the susceptible hosts.

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Application of baculoviruses as biopesticides and the possibilities of nanoparticle mediated delivery

Cited by 7 publications

References 64 publications

A Tale of Two Transcriptomic Responses in Agricultural Pests via Host Defenses and Viral Replication

A Tale of Two Transcriptomic Responses in Agricultural Pests via Host Defenses and Viral Replication

The Effect of Nanozeolite Concentration in a Delivery System of HaNPV1 to the Lethal Time against Crocidolomia pavonana

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

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