Application of biochemical and X-ray diffraction analyses to establish the postmortem interval

Prieto-Castelló, María José; Rincón, Juan Pedro Hernández del; Pérez-Sirvent, Cármen; Álvarez-Jiménez, P.; Pérez-Cárceles, María D.; Osuna, Eduardo; Luna, A.

doi:10.1016/j.forsciint.2006.12.014

Cited by 32 publications

(22 citation statements)

References 14 publications

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“…One can compare the precision here obtained with other methods [1][2][3][4][5][6][7][8][9] applied within the zero to hundred hours PMI determination. In Table 1 we present the precision in hours for conventional methods based on the standard deviation considered the reported experimental data.…”

Section: Resultsmentioning

confidence: 99%

“…This is associated to many features discussed along this text. Although large dispersion is a common characteristic in this area research [1][2][3][4][5][6][7][8][9] .The possibility for a full optical procedure may introduce new advantages.…”

Section: Resultsmentioning

confidence: 99%

“…Besides the possibility of altering the crime scene, these methods are time consuming and cannot be performed in situ, resulting in a delayed and less efficient investigation. Traditional methods for PMI determination present a time precision of approximately 25 hours for the first 100 hours of post-mortem interval [1][2][3][4][5][6][7][8][9][10][11]. Therefore, the development of techniques which enable objective, quantitative and with high time resolution is of great need in forensic medicine.…”

Section: Introductionmentioning

confidence: 99%

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Determination of post-mortem interval using in situ tissue optical fluorescence

Estracanholli¹,

Kurachi²,

Vicente³

et al. 2009

Opt. Express

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Abstract:In this study we have used fluorescence spectroscopy to determine the post-mortem interval. Conventional methods in forensic medicine involve tissue or body fluids sampling and laboratory tests, which are often time demanding and may depend on expensive analysis. The presented method consists in using time-dependent variations on the fluorescence spectrum and its correlation with the time elapsed after regular metabolic activity cessation. This new approach addresses unmet needs for post-mortem interval determination in forensic medicine, by providing rapid and in situ measurements that shows improved time resolution relative to existing methods. References and links 1. P. Vanezis, and O. Trujillo, "Evaluation of hypostasis using a colorimeter measuring system and its application to assessment of the post-mortem interval (time of

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of post-mortem interval using in situ tissue optical fluorescence

Estracanholli¹,

Kurachi²,

Vicente³

et al. 2009

Opt. Express

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“…In addition, there are external factors including air temperature and humidity as well as animal and insect activity. 5,6 Death results in extensive biochemical changes in all body tissues due to absence of circulating oxygen, altered enzymatic reactions, cellular degradation, and cessation of anabolic pathways. These biochemical changes may help to determine the time since death.…”

mentioning

confidence: 99%

Estimation of Early Postmortem Interval Through Biochemical and Pathological Changes in Rat Heart and Kidney

El-Noor

Elhosary²,

Khedr³

et al. 2016

American Journal of Forensic Medicine &Amp; Pathology

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Accurate estimation of time passed since death is a complicated task in forensic medicine especially in homicide or unwitnessed death investigations. Changes in oxidant/antioxidant parameters were investigated if it can be relied upon in estimating the early postmortem interval (EPI) in rat heart and kidney, and whether these changes were correlated with histopathological findings in these tissues. Heart and kidney tissues of 84 male albino rats were divided into 2 parts. One part used for estimation of levels of malondialdehyde (MDA), nitric oxide (NO), and total thiol as well as the activity of glutathione reductase (GR), glutathione S transferase, and catalase. The second part was examined histopathologically. It was found that MDA and NO were significantly increased earlier in the heart than kidney tissues. Meanwhile, total thiol, catalase, glutathione S transferase, and GR were commenced to be significantly decreased in the heart before kidney tissues. Linear regression analysis of independent variables of heart was found to be of a high predictive value of 97.2% (EPI = 8.607 - 0.240 GR + 0.002 MDA + 0.014 NO). Structural deterioration of heart started 3 to 4 hours compared with renal sections that began 5 to 6 hours after death. The relationship between oxidant and antioxidant parameters is crucial in determining the EPI. The kidney was found to be more resistible to oxidative damage. Further research on humans is needed.

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“…Baseado na observação desses fenômenos os principais métodos de determinação do intervalo pós-morte e suas características são citados a seguir (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19).…”

Section: Alguns Métodos Cronotanatognóticos Atuaisunclassified

Determinação do intervalo pós-morte por espectroscopia de fluorescência

Estracanholli¹

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Application of biochemical and X-ray diffraction analyses to establish the postmortem interval

Cited by 32 publications

References 14 publications

Determination of post-mortem interval using in situ tissue optical fluorescence

Determination of post-mortem interval using in situ tissue optical fluorescence

Estimation of Early Postmortem Interval Through Biochemical and Pathological Changes in Rat Heart and Kidney

Determinação do intervalo pós-morte por espectroscopia de fluorescência

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