Application of bioflocculating property of Pseudomonas aeruginosa strain IASST201 in treatment of oil‐field formation water

Abstract

A bioflocculating activity of 89.8% was depicted by an activated sludge-borne bacteria Pseudomonas aeruginosa strain IASST201 with a yield of bioflocculant of 2.68 g L(-1) obtained from production media broth after optimization of different parameters. The highest bioflocculation efficiency was found at the pre-stationary phase of the bacterial growth period in the production media broth at 96th hour examined from a growth-flocculation kinetics study. 85.67% of bioflocculation was observed in oil-field formation water, with a separation of 68.7% of aliphatic hydrocarbon contents of the formation water after the application of the bacterial bioflocculant by entrapment mechanism with formation of flocs which was analyzed and examined comparatively through gas-chromatography. Extensive removal of heavy metal contents of the oil-field formation water due to bioflocculation was estimated by Atomic Absorption Spectrophotometer (AAS). The SEM and AFM studies declare the extracellular polymeric nature of the bioflocculant produced by this bacterium clumped within bacterial biofilm supported with FTIR study of the extracted bioflocculant.

Implications for public health demands alternatives to inorganic and synthetic flocculants: bioflocculants as important candidates

“…The presence of the carboxyl groups on the molecular chain of the biopolymer allows the chain to spread out as a result of electrostatic repulsion and the stretched molecular chains provide more effectual sites for particle attachment (Pathak et al. ). The amino and carboxyl functional groups of bioflocculant can form a complex with heavy metals by neutralizing and stabilizing the residual charge as the binding distance is shortened (Yue et al.…”

“…Due to the extensive capacity of bioflocculants for metals, they are recommended as surface‐active agents for the removal of heavy metals (Pathak et al. ). The physical and chemical properties of the metals, the availability of appropriate binding sites present to metal, as well as the tertiary structure of bioflocculant may all contribute to metal‐binding interactions (Kachlany et al.…”

Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented MethylotrophicMethylobacteriumspp.

“…Unlike organic contaminants, metals are not degradable and remain in the environment for long periods of time; when present at high concentrations in soil, metals can negatively affect plant metabolism, reducing plant growth [ 106 , 107 ]. Therefore, a tolerant microorganism can help to bioremediate contaminated water by flocculation [ 108 ] and soils by heavy metal immobilization [ 109 ], leading to an increase in plant tolerance or even increasing phytoextraction [ 110 ] during bacteria-plant interactions. The potential of the methylotrophic genera is shown by their tolerance to high doses of several heavy metals, such as nickel (Ni), cadmium (Cd), cobalt (Co), zinc (Zn), chrome (Cr) [ 111 ], arsenic (As), lead (Pb) [ 27 ], and mercury (Hg) [ 104 ].…”

Production of a non-cytotoxic bioflocculant by a bacterium utilizing a petroleum hydrocarbon source and its application in heavy metal removal

“…Bioflocculant producing bacteria were isolated from the activated sludge samples following the methodology reported earlier through enrichment culture technique and screened by spread plate technique with selective media agar plates 10 . The isolates were further grown in production media and their bioflocculating activities were determined by flocculating kaolin suspension with the supernatants of the production media broth as reported earlier by Kurane et al 11 .…”