Production of a non-cytotoxic bioflocculant by a bacterium utilizing a petroleum hydrocarbon source and its application in heavy metal removal

Abstract

A bacterium isolated from the activated sludge of an oil refinery of Assam, India retained efficient bioflocculating activity through production of bioflocculant when it was grown on crude oil amended media avoided of any other carbon source.The bioflocculating activity gained from the optimized media broth was 86.2%, which could be enhanced up to 89.1% with the purified bioflocculant. In course of bioflocculant production, the bacterium utilized about 77% of the petroleum hydrocarbons after incubation for 168 h when the activity was found to be the highest. The bioflocculant was efficient in flocculating Ni 2+ , Zn 2+ , Cd 2+ , Cu 2+ and Pb 2+ . The bioflocculant was characterized as a glycoprotein complex by biochemical tests, FT-IR, SEM-EDX and LC/MS analyses. The bioflocculant showed negligible cytotoxicity on testing with L292 cell line indicating tremendous possibility of its use in bioremediation.

Sophorolipid Biosurfactant Can Control Cutaneous Dermatophytosis Caused by Trichophyton mentagrophytes

“…The UPLC-ESI-MS analyses of the sugar components of both the samples confirmed glucose as the sole monomer present, and no other sugars were detected, confirming SL-YS3 to be an SL type of biosurfactant. The mass spectral analyses substantiated the same upon comparison with previous literature (Macášek et al, 2003;Pathak et al, 2015).…”

Development of an Efficient Bacterial Consortium for the Potential Remediation of Hydrocarbons from Contaminated Sites

“…Comparison of the FTIR spectra of the control (untreated crude oil sample) and the test sample (crude oil sample treated with consortium 10 for 5 weeks) distinctly demonstrates the occurrence of degradation process ( Figure 6 ). In juxtaposition of the obtained FTIR spectra discloses the existence of different functional groups which unveils the degradation pattern ( Pathak et al, 2015 ). The control and the test sample display the absorption band of CH 3 , CH 2 , and C = O stretch in similar position (2923, 2824, and 1609 cm -1 for control and 2915, 2854, and 1641 cm -1 for treated sample, respectively).…”

Treatment of crude oil contaminated wastewater via an electrochemical reaction

“…and various toxic heavy metals which can contaminate the natural environment when discharge untreated. 36,37 Thus, in the present study, the different aromatic and aliphatic hydrocarbons and were determined using GC-MS and FTIR analysis. The TPH content of the samples was found to be 1719 AE 54.16 mg L À1 aer the completion of the electrochemical reaction.…”