Application of biomaterials and tissue engineering in bladder regeneration

Wang, Xiaoya; Shi, Chunying; Hou, Xun; Shi, Song; Li, Chenglin; Cao, Weiguo; Chen, Wei; Li, Ling

doi:10.1177/08853282211048574

Cited by 7 publications

(1 citation statement)

References 165 publications

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“…A variety of materials have been explored for bladder engineering including acellular natural materials, synthetic polymers, and naturally derived polymers. 54 Submucosal intestinal submucosa (SIS) and bladder acellular matrix (BAM) are among the most widely explored scaffolds for bladder regeneration, yet these scaffolds present poor mechanical properties for this application. 55 Without the inclusion of cells, associated complications ensue such as brosis and stone formation, none of which were observed in our study.…”

Section: Discussionmentioning

confidence: 99%

Cell-free biodegradable electroactive scaffold for urinary bladder regeneration

Ameer,

Keate,

Bury

et al. 2024

Preprint

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Tissue engineering heavily relies on cell-seeded scaffolds to support the complex biological and mechanical requirements of a target organ. However, in addition to safety and efficacy, translation of tissue engineering technology will depend on manufacturability, affordability, and ease of adoption. Therefore, there is a need to develop scalable biomaterial scaffolds with sufficient bioactivity to eliminate the need for exogenous cell seeding. Herein, we describe synthesis, characterization, and implementation of an electroactive biodegradable elastomer for urinary bladder tissue engineering. To create an electrically conductive and mechanically robust scaffold to support bladder tissue regeneration, we developed a phase-compatible functionalization method wherein the hydrophobic conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was polymerized in situ within a similarly hydrophobic citrate-based elastomer poly(octamethylene-citrate-co-octanol) (POCO) film. We demonstrate the efficacy of this film as a scaffold for bladder augmentation in athymic rats, comparing PEDOT-POCO scaffolds to mesenchymal stromal cell-seeded POCO scaffolds. PEDOT-POCO recovered bladder function and anatomical structure comparably to the cell-seeded POCO scaffolds and significantly better than non-cell seeded POCO scaffolds. This manuscript reports: (1) a new phase-compatible functionalization method that confers electroactivity to a biodegradable elastic scaffold, and (2) the successful restoration of the anatomy and function of an organ using a cell-free electroactive scaffold.

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Section: Discussionmentioning

confidence: 99%

Cell-free biodegradable electroactive scaffold for urinary bladder regeneration

Ameer,

Keate,

Bury

et al. 2024

Preprint

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Out of tissue engineering systemic crisis by means of holographic 3D-photopolymerization

Pulver,

Fofanov,

Pulver

et al. 2024

Discov Biotechnol

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Background Tissue engineering, despite decades of focus on medical applications, has yielded minimal progress in this respect, drastically dissimilar to the avalanche of efforts undertaken. There's no even a conceptual approach, besides recently sprung up "Linear Volumetric Printing". Which, however, has not yet been tested even on biocompatible materials. While existing technologies are limited to creating 3D structures that are either two orders of magnitude larger or three orders of magnitude slower to produce than required for medical-grade bioprinting. Objectives This study proposes a novel approach to overcome these limitations and achieve the creation of fully functional medical grade tissue-engineered constructs. Methods We propose a multi-pronged approach: 1. Define Minimal Requirements: Establish the essential characteristics of bare minimum requirements for fully functional tissue-engineered constructs. 2. Pulsed Holographic Bioprinting: Develop a pulsed holographic submicron-scale photopolymerization system for generating intricate scaffolds (including vascular networks and stromal compartments), based on a mathematical hologram model; 3. Multi-Material Volumetric Printing: Develop a methodology for multi-material volumetric photopolymerization to create complex structures. 4. Cellular Delivery Systems: Design "service conductive dispersion-framework systems" to efficiently deliver cells and bioactive substrates throughout the bioengineered tissue strata, combined with wireframe pseudo-stroma; 5. Gas Transport Culture Medium: Develop a novel gas transport perfusion culture medium for optimal growth and maturation of bioengineered tissues/organs prior to implantation. Expected outcomes We believe that this combined approach will address existing limitations and pave the way for the creation of fully functional bioengineered tissues and organs for medical applications, offering some utterly fantastic prospects.

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