Application of capillary zone electrophoresis to the separation and characterization of poly(amidoamine) dendrimers with an ethylenediamine core

Ebber, A; Vaher, Merike; Peterson, Janek; Lopp, Margus

doi:10.1016/s0021-9673(01)01554-0

Cited by 39 publications

(51 citation statements)

References 18 publications

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“…It is believed that surface charges of dendrimer spheres play an important role in influencing their EMs. It has been demonstrated that CE can be utilized for the separation of different generation of dendrimers and characterization of individual polycationic and polyanionic PAMAM dendrimers [17][18][19][20]. However, to our knowledge, there are no reports specifically related to EM studies of the same-generation PAMAM dendrimers with different functionalities.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges

et al. 2006

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Generation 5 ethylenediamine (EDA)-cored poly(amidoamine) (PAMAM) dendrimers (E5, E denotes the EDA core and 5 the generation number) with different degrees of acetylation and carboxylation were synthesized and used as a model system to investigate the effect of charge and the influence of dendrimer surface modifications on electrophoretic mobility (EM) and molecular distribution. The surface-modified dendrimers were characterized by size-exclusion chromatography, 1H NMR, MALDI-TOF-MS, PAGE, and CE. The focus of our study was to determine how EM changes as a function of particle charge and molecular mass, and how the molecular distribution changes due to surface modifications. We demonstrate that partially modified dendrimers have much broader migration peaks than those of fully surface functionalized or unmodified E5 dendrimers due to variations in the substitution of individual dendrimer surfaces. EM decreased nonlinearly with increases in surface acetylation for both PAMAM acetamides and PAMAM succinamic acids, indicating a complex migration activity in CE separations that is not solely due to charge/mass ratio changes. These studies provide new insights into dendrimer properties under an electric field, as well as into the characterization of dendrimer-based materials being developed for medical applications.

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Section: Introductionmentioning

confidence: 99%

Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges

et al. 2006

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“…Therefore, dendrimer characterization is essential for monitoring the purity of the product. A number of characterization techniques have been applied to PAMAM dendrimers, including PAGE [6,7], CE [5,[7][8][9], HPLC [10], and MALDI [11]. Although CE has become a powerful technique for the characterization of large molecules like proteins and nucleic acids, there is limited literature on its use for dendrimer analysis.…”

Section: Introductionmentioning

confidence: 99%

“…However, higher generations (G4-G5) could not be completely resolved. Under almost identical conditions, other researchers could separate G0-G3 but not G3-G5 [5,8,9]. PAMAM dendrimers were partially separated at neutral pH using a fused-silica capillary and a dynamic coating with 0.05% polyethyleneimine in the electrolyte [12].…”

Section: Introductionmentioning

confidence: 99%

Use of poly(vinyl alcohol)‐coated capillaries for separation of amino‐terminated polyamidoamine dendrimers

2007

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Characterization of amino-terminated polyamidoamine dendrimers by CE suffers from a lack of resolution for higher generations and poor between-day reproducibility of retention times. Under optimal conditions of temperature, voltage, and sample amount, 0-5 generations of dendrimers could be resolved with a bare fused-silica capillary. However, reproducibility was poor due to potential interactions of the polycationic dendrimers with the uncoated quartz capillary wall. Use of a poly(vinyl alcohol)-coated capillary significantly decreased the migration times of the nanomolecules without compromising resolution. Dendrimer mixtures containing generations 0-5 are separated as discrete, nonoverlapping peaks in about 15 min. In addition, the between-day precision of retention times was dramatically improved without the need for internal standards or data normalization. Dendrimers of various generations and cores run on different days showed an RSD of retention times of less than 4%. The poly(vinyl alcohol) coating was very stable as shown by the excellent precision of migration times obtained on a capillary used for a month with more than 100 injections. Similar to PAGE, separation of polyamidoamine dendrimers on a bare fused-silica and poly(vinyl alcohol)-coated capillary showed an exponential relationship between migration times and calculated charge density of the nanomolecules.

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“…34 It was also shown in the literature that polycationic dendrimes are strongly adsorbed on capillary walls. 35 From a large offer of commercially available dendrimers for experiments with modification of BGE the poly[propyleneimine] tetraammonium dendrimer generation 1.0 (DAB) was employed, and as model plant material for obtaining protein profile a commercially available soybean (SB) was employed. The soybean sample was extracted with ACN/water mixture containing 0.3 % acetic acid which was already employed earlier for differentiation of various commercial soybeans.…”

Section: Dendrimers With Amine Core As Bge Modifiersmentioning

confidence: 99%

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

et al. 2011

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Abstract. The capillary electrophoresis with UV detection was employed for protein profiling in extracts from maize and soybeans. Modifications of back-ground electrolyte and coating the capillary wall with polybrene was employed in order to decrease the protein adsorption on the capillary walls. The obtained protein profiles were compared for transgenic and non-transgenic variants, showing in some cases significant changes that might be employed for identification of genetic modifications of plants or foods of plant origin. (doi: 10.5562/cca1777)

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Application of capillary zone electrophoresis to the separation and characterization of poly(amidoamine) dendrimers with an ethylenediamine core

Cited by 39 publications

References 18 publications

Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges

Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges

Use of poly(vinyl alcohol)‐coated capillaries for separation of amino‐terminated polyamidoamine dendrimers

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

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