Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen–antibody interaction

Takeda, Seiji; Sbagyo, Agus; Sakoda, Yoshihiro; Ishii, Atsushi; Sawamura, Makoto; Sueoka, Kazuhisa; Kida, Hiroshi; Mukasa, K.; Matsumoto, Kazuhiko

doi:10.1016/j.bios.2004.08.030

Cited by 36 publications

(21 citation statements)

References 17 publications

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“…The modification of the reverse side of the CNT with NTA-Ni was carried out according to the methods described previously [11]. Fifty μl of 7 μg/ml recombinant protein(s) was dropped on the surface, and incubated for 10 min at room temperature.…”

Section: Modification Of the Reverse Side Of The Cntmentioning

confidence: 99%

“…Formation of the chemical catalyst, CNT growth, and deposition of metallic electrode was carried out according to the methods described previously [11].…”

Section: Fabrication Of Cnt-fet Devicementioning

confidence: 99%

“…Actually, some biological materials including proteins [8], nucleic acids [9], viruses [10], etc, have been detected with CNT-FET based sensors. Recently, we showed that antigen-antiboddy interactions occurring on the reverse side of CNT were detectable with a CNT-FET in extraordinary high sensitivity [11,12]. To extend the availability of our developed CNT-FET sensors for biological use, we attempted the detection of interaction between AChE and insecticides on the reverse side of the CNT.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor

Ishii¹,

Takeda²,

Hattori³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Carbon nanotube sensors were capable of detecting organophosphate insecticide binding to recombinant acetylcholinesterase (rAChE) immobilized on the sensor. The carbon nanotube sensors were fabricated by chemical vapor deposition method and it showed field effect transistor property. rAChE was immobilized by specific binding of NTA-Ni and His tag on the reverse side of the carbon nanotube sensor. The current between the source and drain was measured after incubation of various concentrations of organophosphates with immobilized rAChE. I-V gate curves were obtained by plotting the current as a function of the potential applied to the back gate. The I-V gate curves showed a positive shift in a dose dependent manner, while no shifts were observed when an herbicide was added to the sensor and organophosphates were withdrawn on recombinant calmodulin immobilized surface. In spite of different inhibition effects, two organophosphates showed similar dose response curves in measuring I-V gate correlation with the rAChE-immobilized CNT sensors, suggesting that the binding of organophosphates to rAChE may be sequestered from the inhibition activity of those to the enzyme.

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Section: Modification Of the Reverse Side Of The Cntmentioning

confidence: 99%

“…Formation of the chemical catalyst, CNT growth, and deposition of metallic electrode was carried out according to the methods described previously [11].…”

Section: Fabrication Of Cnt-fet Devicementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor

Ishii¹,

Takeda²,

Hattori³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

Self Cite

View full text Add to dashboard Cite

show abstract

“…The measurements revealed the binding of the monoclonal antibody 10E3, which recognizes U1A specifically, at a concentration of 1 nM or below. CNT-based FETs were also functionalized with hemagglutinins (HAs) [173]. Instead of binding the proteins directly to the nanotubes, they were attached on the reverse side of the silicon wafer ( Fig.…”

Section: Fet Biosensorsmentioning

confidence: 99%

“…An alternative technique consisted on the growth of SWNTs directly on the SiO 2 /Si water by chemical vapor deposition (CVD) [36,[172][173][174][175][176]. Once the SWNTs were deposited, the electrodes were fabricated with electron beam lithography, followed by thermal evaporation and lift-off.…”

Section: Cnts In Fetsmentioning

confidence: 99%