Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review

Bulk, R.W. van den

doi:10.1007/bf00042373

Cited by 93 publications

(25 citation statements)

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“…Several authors have correlated reduced yields and decreased kernel size with mycotoxin production [34,37]. Fundamental knowledge regarding the properties of toxins and their interaction with plant cells (and hence their role in pathogenicity) is, however, generally lacking, making selection for possible resistance difficult [38,39]. Desjardins et al [40] pointed out that the apparent contradictory results regarding the involvement of Fusarium toxins in plant disease may (in part) arise from differing abilities of plants to metabolise the toxins.…”

Section: Introductionmentioning

confidence: 99%

The phytotoxicity ofFusarium metabolites: An update since 1989

McLean

1996

Mycopathologia

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The present article summarises the published phytotoxic effects of severalFusarium metabolites (mycotoxins, phytotoxins, antibiotics and pigments) since 1989. The phytotoxicity of many of the commonly isolated metabolites cannot be disputed, but their role in pathogenesis ofFusarium-induced plant diseases is uncertain. Plant species/varieties differ in their susceptibililty resistance to these toxinsin vitro, as well as toFusarium pathogens under field conditions. Such variations in plant response may reflect resistance mechanisms that operate at several levels, including an initial ability to prevent fungal invasion; prevention of fungal spread and toxin tolerance or degradation. Little is known about the mode of action of most of these metabolites on either animal or plant cells. Several novelFusarium metabolites have been isolated in the past few years. Many are toxic to animals and cell lines, but assessment of their phytotoxicity has largely been neglected. Since many plant pathogenic Fusaria produce a plethora of metabolites, the additive or synergistic actions of toxins in combination must be considered in plant pathology.

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Section: Introductionmentioning

confidence: 99%

The phytotoxicity ofFusarium metabolites: An update since 1989

McLean

1996

Mycopathologia

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“…Other types of chromosomal mutations, e.g. chromosome breakage and chromosome loss have been documented in plants derived from in vitro cultures (Larkin and Scowcroft 1981;Van den Bulk 1991;Bairu et al 2011). We determined the chromosome number to identify aneuploids within R0 plants.…”

Section: Discussionmentioning

confidence: 99%

Response of carrot protoplasts and protoplast-derived aggregates to selection using a fungal culture filtrate of Alternaria radicina

Grzebelus

Kruk

Macko-Podgórni

et al. 2013

Plant Cell Tiss Organ Cult

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Protoplasts isolated from three accessions of cultivated carrot and 5-day-old protoplast-derived aggregates were subjected to selection to identify somaclonal variants with enhanced tolerance to the fungal disease black rot incited by Alternaria radicina. Different concentrations [1, 2, 3.5, 5, 10, 20, 35 and 50 % (v/v)] of a fungal culture filtrate (FCF) from 2-week-old liquid cultures of A. radicina were used. Protoplasts and aggregates were subjected to short-term selection for a period of 10 days. All FCF concentrations added to the cultures on the day of isolation decreased protoplast survival frequency and plating efficiency, while FCF applied 5 days later inhibited cell divisions in 5-50 % concentrations. The responses of protoplasts to the treatment were genotype dependent. Most R0 plants were regenerated in all accessions from cell lines grown with 1 % FCF, while only a few plants were produced from 2 to 3.5 % FCF-treated cultures of 'Dolanka' and the breeding line '9304B', respectively. Nineteen-percent of putative stress-tolerant regenerants were tetraploids, while only 5 % tetraploids were observed in the control. The incidence of unique random amplified polymorphic DNA fragments indicating possible chromosomal rearrangements was low and did not differ among regenerants after selection and those derived from the control. Mobilization of miniature inverted repeat transposable elements was not observed. Some R0 individuals regenerated both from FCF-treated and untreated cultures showed lower susceptibility to A. radicina in a laboratory assay in comparison to control plants grown from seed. Regenerants from FCF-treated cultures showed lower frequency of flowering plants and a higher rate of male sterility. Pollen viability of the putative stress-tolerant regenerants varied over a wide range (6-98 %), independently of in vitro selection conditions. Our data suggest that A. radicina FCF may be feasible for the in vitro selection to generate plants with superior phenotypic performance against A. radicina.

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“…To exploit the concept of resistance breeding, creation of genetic variation with the enhancement of resistance against pathogenic genes is an indispensable part ( Van den Bulk, 1991). In light of these facts, different gene…”

Section: Introductionmentioning

confidence: 99%

Use of Crispr/Cas9 for Development of Disease Resistant Cultivars in Plant Breeding

Ghimire

2017

Int J Appl Sci Biotechnol

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Crop protection against pests and diseases is a major challenge in agriculture. Plant breeding is a key solution for the development of disease resistant cultivars. Gene editing is an indispensable part of plant breeding to obtain desirable traits in crops. CRISPR (Clustered Regular Interspaced Palindromic Repeats)/Cas9 (CRISPR-associated protein) is a recent breakthrough in gene editing technology. It can be utilized to exploit defensive mechanism in plants against pathogen attack with recognition and degradation of the invading pathogenic genes by bacterial immune system. Advances in plant breeding with integration of CRISPR/Cas9 have facilitated the production of cultivars with heritable resistance to viral and bacterial disease. CRISPR/Cas9 mediated genetically engineered resistance can be inherited to further generation of crops after segregation of Cas9/sgRNA transgene in F1 generation. The segregation of Cas9/sgRNA transgene prevents undesirable genome modification in successive generation and makes use of CRISPR/Cas9 safe in plant breeding. CRISPR/Cas9 proves itself as a fascinating tool to revolutionize plant breeding for the development of various disease resistant cultivars however, effects of CRISPR/Cas9 system on different physiological process of plants still need to be studied.

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Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review

Cited by 93 publications

References 97 publications

The phytotoxicity ofFusarium metabolites: An update since 1989

The phytotoxicity ofFusarium metabolites: An update since 1989

Response of carrot protoplasts and protoplast-derived aggregates to selection using a fungal culture filtrate of Alternaria radicina

Use of Crispr/Cas9 for Development of Disease Resistant Cultivars in Plant Breeding

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