Application of cell technologies for production of plant-derived bioactive substances of plant origin

Носов, А. М.

doi:10.1134/s000368381107009x

Cited by 80 publications

(43 citation statements)

References 79 publications

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“…Up-scaling is now technological rather than scientifi c task. Use of plant cell cultures for industrial production of secondary metabolites has been reviewed, but only a few examples of successful commercial process could be found [ 7 ]. Obviously, the main reasons are high cost and demanding technological requirements.…”

Section: Biotechnological Aspects Of Isoprenoid Production From Plantmentioning

confidence: 99%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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Section: Biotechnological Aspects Of Isoprenoid Production From Plantmentioning

confidence: 99%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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“…Dry and moist cell masses were used to characterize the growth and physiological condition of the culture. The viability and cell concentration were determined in a milliliter of medium [4].…”

Section: Methodsmentioning

confidence: 99%

Protodioscin in Dioscorea deltoidea Suspension Cell Culture

et al. 2016

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“…Many NPs, however, have multiple chiral centres with region-specific and stereo-specific properties associated to their function [23], making total chemical synthesis difficult and often unprofitable. Examples include the isoquinoline alkaloids (morphine, codeine, berberine and sanguinarine), isoprenoids (taxanes) and steroid glycosides (ginsenoides) [24]. However, total synthesis of some NPs of high economic importance, such as paclitaxel [25][26][27], vincristine [28] and artemisinin [29,30] has been achieved and constitute important achievements in synthetic chemistry.…”

Section: Production Of Plant Natural Products By Total and Semisynthesismentioning

confidence: 99%

“…An environmentally friendly and renewable source [22]. Higher rates of biomass and metabolite yield can be achieved relative to whole plant sources [24]. NPs produced via plant cell culture can be recognised in the category of Good Manufacturing Practice (GMP) products [42].…”

Section: Advantages Of Plant Cell Culture For Natural Product Synthesismentioning

confidence: 99%

“…There are currently at least 18 plant cell culture companies that produce and commercialise NPs for food and pharmaceutical applications [46]. To date, the two main NPs produced in plant cell culture, Taxol 1 and Vincristine, have an annual market of two and three billions US dollars per year, respectively [24].…”

Section: Advantages Of Plant Cell Culture For Natural Product Synthesismentioning

confidence: 99%

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Cambial meristematic cells: a platform for the production of plant natural products

Ochoa-Villarreal

Howat

Jang³

et al. 2015

New Biotechnology

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Plant cell culture constitutes a sustainable, controllable and environmentally friendly tool to produce natural products for the pharmaceutical, cosmetic and industrial biotechnology industries. However, there are significant obstacles to the commercial synthesis of high value chemicals from plant culture including low yields, performance instability, slow plant cell growth, industrial scale-up and downstream processing. Cambial meristematic cells constitute a platform to ameliorate many of these potential problems enabling the commercial production of high value chemicals.

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Application of cell technologies for production of plant-derived bioactive substances of plant origin

Cited by 80 publications

References 79 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Protodioscin in Dioscorea deltoidea Suspension Cell Culture

Cambial meristematic cells: a platform for the production of plant natural products

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