Application of chlorophyll fluorescence in ecophysiology

Lichtenthaler, Hartmut K.; Buschmann, Claus; Rinderle, U.; Schmuck, G.

doi:10.1007/bf01214643

Cited by 245 publications

(104 citation statements)

References 9 publications

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“…These species are abundant in the High-Arctic tundra in Svalbard, and are also common in the sub-Arctic and alpine environments [35]. The measurements were carried out on green and healthy looking leaves [10,37], resulting in 24, 31, 29 and 28 distinct spectral signatures for Bistorta vivipara, Cassiope tetragona, Dryas octopetala and Salix polaris respectively.…”

Section: In-situ Data Collection and Processingmentioning

confidence: 99%

“…For all analyzed ranges, the results of the NPMANOVA test were significant (p < 0.05). The 400 to 500 nm spectral region allows for an assessment of pigment interaction with light [37,51]. In this region, 12 bands were found to best characterize species that resulted in a 0.90 correctness rate during the LDA stepwise selection of bands.…”

Section: Differences Between Species'mentioning

confidence: 99%

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Intraspecific Differences in Spectral Reflectance Curves as Indicators of Reduced Vitality in High-Arctic Plants

et al. 2017

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Remote sensing is a suitable candidate for monitoring rapid changes in Polar regions, offering high-resolution spectral, spatial and radiometric data. This paper focuses on the spectral properties of dominant plant species acquired during the first week of August 2015. Twenty-eight plots were selected, which could easily be identified in the field as well as on RapidEye satellite imagery. Spectral measurements of individual species were acquired, and heavy metal contamination stress factors were measured contemporaneously. As a result, a unique spectral library of dominant plant species, heavy metal concentrations and damage ratios were achieved with an indication that species-specific changes due to environmental conditions can best be differentiated in the 1401-2400 nm spectral region. Two key arctic tundra species, Cassiope tetragona and Dryas octopetala, exhibited significant differences in this spectral region that were linked to a changing health status. Relationships between field and satellite measurements were comparable, e.g., the Red Edge Normalized Difference Vegetation Index (RENDVI) showed a strong and significant relationship (R 2 = 0.82; p = 0.036) for the species Dryas octopetala. Cadmium and Lead were below detection levels while manganese, copper and zinc acquired near Longyearbyen were at concentrations comparable to other places in Svalbard. There were high levels of nickel near Longyearbyen (0.014 mg/g), while it was low (0.004 mg/g) elsewhere.

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Section: In-situ Data Collection and Processingmentioning

confidence: 99%

Section: Differences Between Species'mentioning

confidence: 99%

Intraspecific Differences in Spectral Reflectance Curves as Indicators of Reduced Vitality in High-Arctic Plants

et al. 2017

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“…After determination of f, wt plant material was frozen and lyophilized. For pigment analysis, chlorophyll a and b and carotenoids of 12 samples per treatment were extracted with DMSO according Hiscox & Israelstam (1979) and calculated according to Lichtenthaler (1987), Carbohydrate content was determined as follows: samples of c. 50 mg of lyophilized material were extracted with 10 mg Polyclar AT (Serva) and 1 -5 ml of hot water in a 2 ml vial at 80 °C for 15 min. After cooling, the samples were shaken for 20 min and centrifuged, the pellet was then washed twice with distilled water, supernatants pooled, lyophilized, and stored at -25 °C,…”

Section: Sampling and Assaysmentioning

confidence: 99%

Physiological changes on agricultural crops induced by different ambient ozone exposure regimes

et al. 1997

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(This paper is dedicated to Professor Andreas Sievers on the occasion of his retirement) SUMMARY Spring wheat (Triticiim aestivum cv, Nandu) culti\ated under glasshouse conditions was exposed to ozone in large fumigation chambers for 2 wk. Different exposure regimes were applied as constant concentrations as well as with ozone peaks, parth' under equal dose-conditions, in times of high solar radiation during different stages of development (seedling, late tillering, anthesis). Chlorophyll fluorescence was monitored and amounts of carbohydrates (hexoses, sucrose, starch) and chlorophyll were measured in young leaves (seedling) and flag leaves (late tillering, anthesis) during and after ozone exposure. Although seedlings showed no significant response in photosynthesis, strong effects on photosynthesis and carbohydrate accumulation were measured when plants were fumigated during anthesis, especially after a heat stress period preceding ozone treatments. Under equal dose conditions chlorophyll fluorescence parameters (F.^.\F^^ and electron transport rate decreased and sucrose content of flag leaves increased significantly if ozone at a concentration of 220 //g rrr'^ was supplied for 4 h, indicating that peak concentrations show stronger effects than constant concentrations. The reaction of wheat plants is dependent on environmental conditions such as preceding heat stress and on the developmental stage during exposure. The results favour the h\ pothesis that photoinhibition and disturbance of photosynthesis are only secondary effects as a consequence of retarded sucrose export from the leaf, because of damage at the plasma membrane.

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“…Various studies were conducted on the active measurement of ChlF signals using single point measurements [7][8][9][10][11][12] or ChlF imaging [13][14][15][16][17][18][19][20][21][22][23]. The ChlF principle is based on how light energy, which is absorbed by photosynthetic pigments such as chlorophylls and cartenoids is distributed [10].…”

Section: Introductionmentioning

confidence: 99%

Estimating Chlorophyll Fluorescence Parameters Using the Joint Fraunhofer Line Depth and Laser-Induced Saturation Pulse (FLD-LISP) Method in Different Plant Species

2017

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Abstract:A comprehensive evaluation of the recently developed Fraunhofer line depth (FLD) and laser-induced saturation pulse (FLD-LISP) method was conducted to measure chlorophyll fluorescence (ChlF) parameters of the quantum yield of photosystem II (Φ PSII ), non-photochemical quenching (NPQ), and the photosystem II-based electron transport rate (ETR) in three plant species including paprika (C3 plant), maize (C4 plant), and pachira (C3 plant). First, the relationships between photosynthetic photon flux density (PPFD) and ChlF parameters retrieved using FLD-LISP and the pulse amplitude-modulated (PAM) methods were analyzed for all three species. Then the relationships between ChlF parameters measured using FLD-LISP and PAM were evaluated for the plants in different growth stages of leaves from mature to aging conditions. The relationships of ChlF parameters/PPFD were similar in both FLD-LISP and PAM methods in all plant species. Φ PSII showed a linear relationship with PPFD in all three species whereas NPQ was found to be linearly related to PPFD in paprika and maize, but not for pachira. The ETR/PPFD relationship was nonlinear with increasing values observed for PPFDs lower than about 800 µmol m −2 s −1 for paprika, lower than about 1200 µmol m −2 s −1 for maize, and lower than about 800 µmol m −2 s −1 for pachira. The Φ PSII , NPQ, and ETR of both the FLD-LISP and PAM methods were very well correlated (R 2 = 0.89, RMSE = 0.05), (R 2 = 0.86, RMSE = 0.44), and (R 2 = 0.88, RMSE = 24.69), respectively, for all plants. Therefore, the FLD-LISP method can be recommended as a robust technique for the estimation of ChlF parameters.Keywords: C3 plant; C4 plant; Fraunhofer line depth and laser-induced saturation pulse (FLD-LISP) method; non-photochemical quenching (NPQ); photochemical yield of photosystem II (Φ PSII ); photosystem II-based electron transport rate (ETR); solar-induced chlorophyll fluorescence (SIF)

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Application of chlorophyll fluorescence in ecophysiology

Cited by 245 publications

References 9 publications

Intraspecific Differences in Spectral Reflectance Curves as Indicators of Reduced Vitality in High-Arctic Plants

Intraspecific Differences in Spectral Reflectance Curves as Indicators of Reduced Vitality in High-Arctic Plants

Physiological changes on agricultural crops induced by different ambient ozone exposure regimes

Estimating Chlorophyll Fluorescence Parameters Using the Joint Fraunhofer Line Depth and Laser-Induced Saturation Pulse (FLD-LISP) Method in Different Plant Species

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