Application of compact porous tubes for preparative isolation of clotting factor VIII from human plasma

Preparation of Factor VIII Using an Ion Exchange Medium CaptoTM MMC

“…Additionally, the high specific activity obtained should be ascribed mainly to the reduction of high molecular weight proteins, such as fibrinogen, fibronectin and immunoglobulins. The results of this study were consistent with several domestic reports, but the specific activity was lower than the previous publications [6,7] .…”

Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns

“…One of the important features of CIMm monolithic columns was shown to be flow-unaffected dynamic capacity up to 8 mL/min [23]. In this work we extended this range up to 46.5 mL/min which is equal to 2467 cm/h.…”

“…As can be seen from Figure 1 the shapes and positions Besides batch-to-batch reproducibility, long-term stability is another essential feature of every chromatographic support. Stability in terms of constant separation quality was already demonstrated with unchanged separation over 200 injections [23]. In this work we aimed to test long-term stability by measuring the dynamic binding capacity change during several loadings.…”

“…CIM (Convective Interaction Media)m supports are commercially available glycidyl methacrylate based monoliths. They enable very fast separation and purification of proteins [18 -22] and exhibit flow-unaffected resolution and dynamic binding capacity [23]. The latter is especially important for the purification of molecules on a preparative scale where productivity is essential.…”

Purification of Factor VIII From Cryoprecipitation Using MacroCapTM Q Column

“…Another, low-molecular-weight (LMW) component retarded by the gel and possesses the bulk of procoagulant activity, but is relatively poor in proteins [4]. Controlled pore glass (500 Å pore diameter) [5], compact porous tubes [6] as well as size-exclusion columns [7] are used for large-scale purification of FVIII. In latest studies, anion exchange chromatography is found to be good at large-scale purification of FVIII, and the specific activity is greater than 300 IU/mg protein.…”