Application of compound material alleviates saline and alkaline stress in cotton leaves through regulation of the transcriptome

Abstract

Background Soil salinization and alkalinization are the main factors that affect the agricultural productivity. Evaluating the persistence of the compound material applied in field soils is an important part of the regulation of the responses of cotton to saline and alkaline stresses. Result To determine the molecular effects of compound material on the cotton’s responses to saline stress and alkaline stress, cotton was planted in the salinized soil (NaCl 8 g kg− 1) and alkalized soil (Na2CO3 8 g kg− 1) after application of the compound material, and ion content, physiological characteristics, and transcription of new cotton leaves at flowering and boll-forming stage were analyzed. The results showed that compared with saline stress, alkaline stress increased the contents of Na+, K+, SOD, and MDA in leaves. The application of the compound material reduced the content of Na+ but increased the K+/Na+ ratio, the activities of SOD, POD, and CAT, and REC. Transcriptome analysis revealed that after the application of the compound material, the Na+/H+ exchanger gene in cotton leaves was down-regulated, while the K+ transporter, K+ channel, and POD genes were up-regulated. Besides, the down-regulation of genes related to lignin synthesis in phenylalanine biosynthesis pathway had a close relationship with the ion content and physiological characteristics in leaves. The quantitative analysis with PCR proved the reliability of the results of RNA sequencing. Conclusion These findings suggest that the compound material alleviated saline stress and alkaline stress on cotton leaves by regulating candidate genes in key biological pathways, which improves our understanding of the molecular mechanism of the compound material regulating the responses of cotton to saline stress and alkaline stress.

A Gγ protein regulates alkaline sensitivity in crops

“…Huili Zhang 1,2 †, Feifei Yu 1,3 †*, Peng Xie 1 †, Shengyuan Sun 4,5 †, Xinhua Qiao 6 †, Sanyuan Tang 1 , Chengxuan Chen 1 , Sen Yang 1,7 , Cuo Mei 1,7 , Dekai Yang 1,7 , Yaorong Wu 1 , Ran Xia 1 , Xu Li 4 , Jun Lu 4 , Yuxi Liu 4 , Xiaowei Xie 2 , Dongmei Ma 2 , Xing Xu 2 , Zhengwei Liang 8 , Zhonghui Feng 7,8 , Xiahe Huang 1 , Hong Yu 1 , Guifu Liu 1 , Yingchun Wang 1,7 , Jiayang Li 1,7 , Qifa Zhang 4 , Chang Chen 6,7 , Yidan Ouyang 4 *, Qi Xie 1,7,9 * The use of alkaline salt lands for crop production is hindered by a scarcity of knowledge and breeding efforts for plant alkaline tolerance. Through genome association analysis of sorghum, a naturally high-alkaline-tolerant crop, we detected a major locus, Alkaline Tolerance 1 (AT1), specifically related to alkaline-salinity sensitivity.…”

“…High alkalinity boosts hydrogen peroxide (H 2 O 2 ) levels in cells and causes oxidative damage to cellular proteins, lipids, and DNA, which results in apoptosis ( 5 – 7 ). Aquaporins (AQPs) are conserved from prokaryotes to eukaryotes.…”

Multi-Omics Analysis of the Effects of Soil Amendment on Rapeseed (Brassica napus L.) Photosynthesis under Drip Irrigation with Brackish Water

“…PPM is an independently developed soil amendment with inorganic and organic polymers as the main components. It has high-water solubility, and can be applied through drip irrigation [ 15 ]. Studies have shown that polyacrylamide has no significant negative effects on aquatic ecosystems and soil organisms when used at recommended doses (10–20 kg·hm −2 ) [ 16 , 17 ].…”

“…The proper use of manganese sulfate avoids manganese deficiency and thus enhances plant growth and resistance, and generally the safe concentration of manganese sulphate solution is 0.1–1% [ 19 , 20 ]. Our previous study found that PPM had a variety of functional groups, such as carboxyl, alcohol hydroxyl, and silicone, whose main effect is to improve the soil structure and buffer saline ions in the soil [ 15 , 21 ]. The application of PPM could improve the resistance of cotton, wheat, and other crops to salt stress [ 15 ].…”

“…Our previous study found that PPM had a variety of functional groups, such as carboxyl, alcohol hydroxyl, and silicone, whose main effect is to improve the soil structure and buffer saline ions in the soil [ 15 , 21 ]. The application of PPM could improve the resistance of cotton, wheat, and other crops to salt stress [ 15 ]. B. napus is a salt-tolerant plant.…”