Application of computer-assisted sperm analysis system to elucidate lack of effects of cyclophosphamide on rat epididymal sperm motion.

Higuchi, Hashihiro; Nakaoka, M.; Kawamura, Satoshi; Kamita, Yusuke; Kohda, Akira; Seki, Takaki

doi:10.2131/jts.26.75

Cited by 23 publications

(11 citation statements)

References 17 publications

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“…CP-induced damage to spermatogenesis is most directly refl ected by sperm count, survival ratio, and abnormality ratio. Previous studies reported that long-term low-dose CP exposure should result in reductions in body weight, testis weight, and testis index in humans or animals (17), and reduction of sperm count and quality (18), which would affect growth of offspring and increase the percentage of deformities therein. Testis weight is connected with quantities of different differentiational spermatids (19).…”

Section: Discussionmentioning

confidence: 99%

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Le¹,

Luo²,

Gu³

et al. 2015

BLL

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Cyclophosphamide (CP) is a commonly used antitumour and immunosuppressive drug, but it is inevitable that the chemotherapeutic agent may cause long-term or permanent reproductive damage on young male patients through inducing oxidative stress in the testes. Squid ink polysaccharides (SIP), a newly found marine glycosaminoglycon have been proved to have antioxidant capabilities and chemotherapy-protective activities on model animals in our recent investigations. This study was conducted to assess whether or not SIP could protect male mice against gonadotoxicity during CP exposure. Sexually mature male Kunming mice were allocated to one of four groups. CP was abdominally administered at dose of 15 mg/kg body weight to two groups of mice for ten weeks, once a week, one group of mice received SIP at dose of 80 mg/kg body weight by gavage for ten weeks, once a day. The other two groups comprised a vehicle treated group and an SIP treated group. Toxicity of CP and protective activity of SIP on the testes were assessed by: sperm parameters, organ index, testicular antioxidant ability, activities of marker enzymes, sex hormone content, and histopathological features. Data showed CP-induced, serious negative changes on murine sperm parameters, organ index, testicular antioxidant ability, activities of marker enzymes, sexual hormone contents, and histopathological features which were all signifi cantly impaired by SIP. This study found that SIP were demonstrated to offer protective effects against CP-induced toxicity on testes in mice (Tab. 2, Fig. 3, Ref. 29). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 99%

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Le¹,

Luo²,

Gu³

et al. 2015

BLL

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“…These considerations suggest that the % motile sperm and sperm velocity parameters can be important indicators for assessment of male fertility. On the other hand, decreases in number of implantations and live embryos without motion and concentration disorders of spermatozoa were induced by CP (Higuchi et al, 2001), and they concluded that sperm motion analysis is an evaluation of sperm mobility but not an indicator of sperm maturity. Our group, therefore, concluded that accurate evaluation of male reproductive function requires appropriate assays, such as sperm examination (motion analysis, concentration in testis or epididymis, and morphology), reproductive organ findings (weights and histopathology), and fertility tests (mating trials and reproductive status).…”

Section: Straightness (Str) and Linearity (Lin)mentioning

confidence: 99%

“…This report was compiled from studies on adriamycin (Kato et al, 2001), -chlorohydrin (Fukunishi et al, 1998;Ikegawa et al, 1997;Kato et al, 1999), cyclophosphamide (Higuchi et al, 2001), ethylene glycol monoethyl ether (Horimoto et al, 2000a), dinoseb (Sato et al, 2000), 2,5-hexanedione (Horimoto et al, 2000a), sulfasalazine (Horimoto et al, 2000a), trimethyl phosphate (Fukunishi et al, 1999), and ornidazole (Horimoto et al, 2000b).…”

Section: Introductionmentioning

confidence: 99%

Overview of studies on rat sperm motion analysis using a Hamilton-Thorne sperm analyzer. Collaborative working study.

Kato¹,

Fukunishi²,

Ikegawa

et al. 2001

J. Toxicol. Sci.

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This collaborative study was conducted to determine the utility and sensitivity of nine sperm motion parameters generated by a Hamilton-Thorne Sperm Analyzer (HTM-IVOS) for detecting adverse effects of chemicals on sperm motion in rats. The efficacy of sperm motion parameters was investigated using nine reproductive toxicants: adriamycin, -chlorohydrin (3 different studies were carried out), dinoseb, ethylene glycol monoethyl ether, 2,5-hexanedione, sulfasalazine, trimethyl phosphate, and ornidazole.The percentage of motile sperm (% motile sperm), the only parameter expressing the status of semen containing non-motile sperm, detected adverse effects on sperm motion in 9 out of 10 studies. However, weak effects on sperm motion were not detected by this parameter in 4 out of 7 studies in which sperm motion disorders were noted at medium or low dosages. The percentage of progressively motile sperm (% progressive sperm) and the sperm velocity parameters (average path velocity, straight line velocity, and curvilinear velocity) detected adverse effects on sperm motion in all studies. In 7 studies which noted sperm motion disorders at medium or low dosages, weak effects on sperm motion were detected by the % progressive sperm in 5 studies and by the sperm velocity parameters in 6 studies. In 10 studies, amplitude of lateral head displacement (ALH) did not detect adverse effects on sperm motion in 4 studies, and beat cross frequency (BCF) failed to detect adverse effects on sperm motion in 3 studies. Because ALH and BCF show the swimming pattern of spermatozoa as head movement, the characteristics of these parameters are different from the % progressive sperm and the sperm velocity parameters. Straightness (STR) and linearity (LIN), which are secondary parameters calculated from sperm velocity parameters, could not detect adverse effects on sperm motion when the sperm velocity parameters did not detect adverse effects.On the basis of these results, we concluded that the % progressive sperm and sperm velocity parameCorrespondence : Masashi KATO M. KATO et al.

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“…Studies with alkylating agents, such as ethyl methanesulphonate (Takagi et al, 2000) and cyclophosphamide (Higuchi et al, 2001), have also failed to detect the relationship between dominant lethals and sperm examinations. These negative consistencies among the studies with alkylating agents indicate that it may be difficult to detect sperm conditions that are potentially dominant lethal.…”

Section: Discussionmentioning

confidence: 99%

A Time-Course Characterization of Male Reproductive Toxicity in Rats Treated With Methyl Methanesulphonate (Mms)

et al. 2005

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-Methyl methanesulphonate (MMS), a potent alkylating agent and testicular toxicant, was orally administered to rats for 5 days at doses of 20, 30, and 40 mg/kg. During the recovery period of 5 weeks, males were evaluated for multiple endpoints such as organ weights, fertility, and sperm parameters. The 5-week recovery periods are designated as follows: Day 1 (1 day after final treatment); Week 1, Week 2, Week 3, Week 4, and Week 5 (first, second, third, fourth, and fifth week after final treatment). A clear time-course of dominant lethals was observed. The peak severities of the dominant lethals were observed in Week 2. It was judged that the most sensitive cellular targets for the dominant lethals are late spermatids. Sperm examination revealed a clear time-course and dose-dependent changes in the frequency of sperm morphological abnormalities. The peak severities of the sperm morphological alterations in cauda epididymis were observed in Week 4. Sensitive cellular stages for the induction of sperm morphological abnormalities were judged to be late spermatocytes and early spermatids. The most frequently observed type of morphologically abnormal spermatozoa was tailless sperm, followed by no-hook head sperm. Although the initial cause for both sperm morphological alterations and dominant lethals was suggested to be genetic insult to the germ cells, there were no obvious relationships observed between these two findings.

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Application of computer-assisted sperm analysis system to elucidate lack of effects of cyclophosphamide on rat epididymal sperm motion.

Cited by 23 publications

References 17 publications

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Overview of studies on rat sperm motion analysis using a Hamilton-Thorne sperm analyzer. Collaborative working study.

A Time-Course Characterization of Male Reproductive Toxicity in Rats Treated With Methyl Methanesulphonate (Mms)

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