Application of conductive PPy/SF composite scaffold and electrical stimulation for neural tissue engineering

Zhao, Ying‐Zheng; Liang, Yunyun; Ding, Supeng; Zhang, Kunyu; Mao, Hai‐Quan; Yang, Yumin

doi:10.1016/j.biomaterials.2020.120164

Cited by 200 publications

(167 citation statements)

References 46 publications

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“…According to the previous work, mitogen‐activated protein kinase (MAPK) and extracellular signal‐regulated kinase (ERK) signal transduction pathways can be activated by ES at the conductive conduit for guiding peripheral nerve regeneration. [ 36 ] In detail, as cells sensed the external ES by electrocoupling, transduction of electrical signals to biochemical cues activated intracellular post‐translational/transcriptional responses and governed multiple cellular pathways through decreasing the expression of p38 MAPK and/or pERK. Furthermore, findings demonstrated that an orientated scaffold with uniform longitudinal topographical cues displayed promoted migration of SCs, axonal regeneration, and functional recovery due to the contact‐guidance effect as well as the protection for regenerating axons from compression stress.…”

Section: Resultsmentioning

confidence: 99%

Conductive Composite Fiber with Optimized Alignment Guides Neural Regeneration under Electrical Stimulation

Jin

Zhang

Wang

et al. 2020

Adv Healthcare Materials

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Conductivity and alignment of scaffolds are two primary factors influencing the efficacy of nerve repair. Herein, conductive composite fibers composed of poly(ɛ-caprolactone) (PCL) and carbon nanotubes (CNTs) with different orientation degrees are prepared by electrospinning at various rotational speeds (0, 500, 1000, and 2000 rpm), and meanwhile the synergistic promotion mechanism of aligned topography and electrical stimulation on neural regeneration is fully demonstrated. Under an optimized rotational speed of 1000 rpm, the electrospun PCL fiber exhibits orientated structure at macroscopic (mean deviation angle = 2.78°) or microscopic crystal scale (orientation degree = 0.73), decreased contact angle of 99.2°± 4.9°, and sufficient tensile strength in both perpendicular and parallel directions to fiber axis (1.13 ± 0.15 and 5.06 ± 0.98 MPa). CNTs are introduced into the aligned fiber for further improving conductivity (15.69-178.63 S m −1), which is beneficial to the oriented growth of neural cells in vitro as well as the regeneration of injured sciatic nerves in vivo. On the basis of robust cell induction behavior, optimum sciatic nerve function index, and enhanced remyelination/axonal regeneration, such conductive PCL/CNTs composite fiber with optimized fiber alignment may serve as instructive candidates for promoting the scaffold-and cell-based strategies for neural repair.

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Section: Resultsmentioning

confidence: 99%

Conductive Composite Fiber with Optimized Alignment Guides Neural Regeneration under Electrical Stimulation

Jin

Zhang

Wang

et al. 2020

Adv Healthcare Materials

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“…Additionally, c-Jun N-terminal kinases (JNKs) is known to regulate inflammatory responses, axonal regeneration and myelination. Zhao et al clarified that electrical stimulation at electroconductive scaffolds led to the activation of MAPKs and the attenuation of JNKs in the regenerated peripheral nerves [ 74 ].…”

Section: The Interaction Between Rgo and Stem Cells In Peripheral Nerve Regenerationmentioning

confidence: 99%

The influence of reduced graphene oxide on stem cells: a perspective in peripheral nerve regeneration

Yao

Yan

Wang

et al. 2021

Regenerative Biomaterials

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Graphene and its derivatives are fascinating materials for their extraordinary electrochemical and mechanical properties. In recent decades, many researchers explored their applications in tissue engineering and regenerative medicine. Reduced graphene oxide (rGO) possesses remarkable structural and functional resemblance to graphene, although some residual oxygen-containing groups and defects exist in the structure. Such structure holds great potential since the remnant-oxygenated groups can further be functionalized or modified. Moreover, oxygen-containing groups can improve the dispersion of rGO in organic or aqueous media. Therefore, it is preferable to utilize rGO in the production of composite materials. The rGO composite scaffolds provide favorable extracellular microenvironment and affect the cellular behavior of cultured cells in the peripheral nerve regeneration. On the one hand, rGO impacts on Schwann cells and neurons which are major components of peripheral nerves. On the other hand, rGO-incorporated composite scaffolds promote the neurogenic differentiation of several stem cells, including embryonic stem cells, mesenchymal stem cells, adipose-derived stem cells and neural stem cells. This review will briefly introduce the production and major properties of rGO, and its potential in modulating the cellular behaviors of specific stem cells. Finally, we present its emerging roles in the production of composite scaffolds for nerve tissue engineering.

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“…Others have incorporated polypyrrole, a conductive polymer, into their scaffolds to improve electrical properties. [ 93–95 ] This has been achieved by direct inkjet printing of polypyrrole and collagen [ 93 ] or via using a stereolithography based 3D bioprinter to form silk fibroin scaffolds that were subsequently coated with polypyrrole via electrospinning. [ 94,95 ] The electrical resistivity of these scaffolds were measured using a two‐ or four‐probe method with a digital multimeter.…”

Section: Introductionmentioning

confidence: 99%

“…[ 93–95 ] This has been achieved by direct inkjet printing of polypyrrole and collagen [ 93 ] or via using a stereolithography based 3D bioprinter to form silk fibroin scaffolds that were subsequently coated with polypyrrole via electrospinning. [ 94,95 ] The electrical resistivity of these scaffolds were measured using a two‐ or four‐probe method with a digital multimeter. This data was then used to estimate the conductivity of the scaffolds.…”

Section: Introductionmentioning

confidence: 99%

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3D Bioprinting of Neural Tissues

Cadena

Ning

King

et al. 2020

Adv Healthcare Materials

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The human nervous system is a remarkably complex physiological network that is inherently challenging to study because of obstacles to acquiring primary samples. Animal models offer powerful alternatives to study nervous system development, diseases, and regenerative processes, however, they are unable to address some species‐specific features of the human nervous system. In vitro models of the human nervous system have expanded in prevalence and sophistication, but still require further advances to better recapitulate microenvironmental and cellular features. The field of neural tissue engineering (TE) is rapidly adopting new technologies that enable scientists to precisely control in vitro culture conditions and to better model nervous system formation, function, and repair. 3D bioprinting is one of the major TE technologies that utilizes biocompatible hydrogels to create precisely patterned scaffolds, designed to enhance cellular responses. This review focuses on the applications of 3D bioprinting in the field of neural TE. Important design parameters are considered when bioprinting neural stem cells are discussed. The emergence of various bioprinted in vitro platforms are also reviewed for developmental and disease modeling and drug screening applications within the central and peripheral nervous systems, as well as their use as implants for in vivo regenerative therapies.

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Application of conductive PPy/SF composite scaffold and electrical stimulation for neural tissue engineering

Cited by 200 publications

References 46 publications

Conductive Composite Fiber with Optimized Alignment Guides Neural Regeneration under Electrical Stimulation

Conductive Composite Fiber with Optimized Alignment Guides Neural Regeneration under Electrical Stimulation

The influence of reduced graphene oxide on stem cells: a perspective in peripheral nerve regeneration

3D Bioprinting of Neural Tissues

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